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The map label for this gene is rnjA

Identifier: 16078517

GI number: 16078517

Start: 1523118

End: 1524785

Strand: Reverse

Name: rnjA

Synonym: BSU14530

Alternate gene names: 16078517

Gene position: 1524785-1523118 (Counterclockwise)

Preceding gene: 16078518

Following gene: 16078511

Centisome position: 36.17

GC content: 44.0

Gene sequence:

>1668_bases
ATGAAATTTGTAAAAAATGATCAGACTGCTGTTTTCGCCCTCGGAGGTTTGGGCGAAATCGGTAAAAATACGTACGCGGT
GCAATTCCAGGATGAAATTGTACTGATTGATGCGGGGATCAAGTTTCCCGAGGACGAACTGCTCGGAATTGACTACGTCA
TCCCTGATTATACGTATTTAGTCAAAAACGAAGATAAAATTAAAGGGCTTTTTATCACCCACGGGCACGAAGACCACATC
GGCGGTATTCCATACCTGCTCAGACAAGTGAATATTCCTGTATACGGCGGAAAACTCGCGATCGGCTTACTTCGGAATAA
ACTTGAAGAGCATGGATTATTGAGACAGACGAAACTGAACATTATCGGCGAGGATGATATTGTAAAGTTCCGCAAAACGG
CTGTGTCATTCTTCAGAACAACACACAGTATTCCGGATTCCTACGGCATTGTTGTAAAAACGCCGCCCGGAAACATCGTA
CATACTGGGGATTTCAAATTCGACTTTACTCCAGTCGGCGAGCCGGCAAACTTAACGAAAATGGCCGAAATCGGTAAAGA
AGGCGTTTTGTGCCTTCTTTCAGACAGCACAAACAGTGAAAATCCGGAGTTTACCATGTCTGAGCGCCGTGTAGGAGAAA
GCATTCATGACATTTTCCGCAAGGTGGACGGCCGAATTATCTTCGCCACATTTGCGTCGAATATTCACCGCTTGCAGCAG
GTAATTGAAGCTGCTGTACAAAATGGAAGAAAAGTTGCCGTATTTGGACGCAGTATGGAATCGGCTATCGAAATTGGACA
GACACTCGGTTATATTAACTGTCCTAAAAATACGTTTATTGAGCATAACGAAATCAATCGTATGCCTGCTAATAAAGTAA
CAATTTTATGTACAGGAAGCCAAGGGGAACCAATGGCGGCGTTATCAAGAATTGCAAACGGCACACACCGCCAAATTTCA
ATCAATCCTGGGGATACAGTCGTATTTTCTTCATCCCCTATCCCAGGCAACACAATCAGCGTGAGTCGAACAATCAACCA
GCTGTATCGTGCGGGTGCTGAGGTTATTCACGGCCCTCTTAACGATATCCATACATCCGGTCACGGCGGACAGGAAGAAC
AGAAGCTGATGCTTCGTTTAATCAAGCCTAAATTCTTCATGCCGATTCACGGTGAGTACAGAATGCAAAAGATGCATGTC
AAACTTGCAACAGATTGTGGCATCCCAGAGGAAAACTGCTTTATCATGGATAATGGTGAAGTATTAGCACTTAAAGGCGA
TGAGGCTTCAGTTGCAGGAAAAATCCCGTCCGGTTCAGTGTACATTGACGGAAGCGGTATCGGTGATATTGGCAATATCG
TACTTCGTGATCGCAGAATTCTCTCTGAAGAAGGACTTGTCATCGTTGTTGTCAGCATTGACATGGACGACTTCAAGATT
TCAGCGGGTCCTGATTTGATTTCCAGAGGATTTGTGTACATGAGAGAATCTGGTGACTTGATCAACGACGCTCAAGAGCT
CATTTCAAATCACTTACAAAAAGTAATGGAACGAAAAACGACTCAATGGTCTGAAATCAAAAACGAAATTACAGACACGC
TTGCACCTTTCTTGTACGAAAAAACAAAGCGCCGTCCAATGATCCTGCCGATCATTATGGAGGTTTAA

Upstream 100 bases:

>100_bases
AAAAGAAATTTAATATTGAGTTTATTACACCTGTTGATGGTGCATTCTTAGAATATGAACAACAGAGCGAAAATTTTAAA
GTATTGGAGTTATGAGCGGT

Downstream 100 bases:

>100_bases
AAAAAGAGGCACTCCCTAAGGGAGTGCCTCTTTTTATTGCAGTGATATGTGTTGAAATGATTTTACATCGAATAATCCCG
TAGTCGTCATTTTAATATCA

Product: ribonuclease J1

Products: NA

Alternate protein names: RNase J1

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHI
GGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIV
HTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQ
VIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQIS
INPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHV
KLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKI
SAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV

Sequences:

>Translated_555_residues
MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHI
GGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIV
HTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQ
VIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQIS
INPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHV
KLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKI
SAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV
>Mature_555_residues
MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYLVKNEDKIKGLFITHGHEDHI
GGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIV
HTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQ
VIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPMAALSRIANGTHRQIS
INPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHV
KLATDCGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKI
SAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNJ1_BACSU (Q45493)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   B69862
- RefSeq:   NP_389336.1
- ProteinModelPortal:   Q45493
- SMR:   Q45493
- DIP:   DIP-46392N
- EnsemblBacteria:   EBBACT00000003508
- GeneID:   939483
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14530
- NMPDR:   fig|224308.1.peg.1455
- GenoList:   BSU14530
- GeneTree:   EBGT00050000000394
- HOGENOM:   HBG497378
- OMA:   THRQISI
- ProtClustDB:   CLSK873383
- BioCyc:   BSUB:BSU14530-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587
- SMART:   SM00849
- TIGRFAMs:   TIGR00649

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL

EC number: NA

Molecular weight: Translated: 61518; Mature: 61518

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYL
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEEEECCCCCCCHHHEEEEEECCCEEEE
VKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLN
EECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCEEEEEEE
IIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPVGEPANLTK
EECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCEEEEECCCCCCCHHH
MAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQ
HHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHH
VIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPL
CCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHHCCHHHEECCH
NDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGE
HHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCCCCCEEEEECCC
VLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKI
EEEEECCCCEEECCCCCCCEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEECCCCEEE
SAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYE
ECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEV
HHCCCCEEEEEEECC
>Mature Secondary Structure
MKFVKNDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIPDYTYL
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEEEECCCCCCCHHHEEEEEECCCEEEE
VKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLRNKLEEHGLLRQTKLN
EECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCEEEEEEE
IIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTPPGNIVHTGDFKFDFTPVGEPANLTK
EECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCEEEEECCCCCCCHHH
MAEIGKEGVLCLLSDSTNSENPEFTMSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQ
HHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHH
VIEAAVQNGRKVAVFGRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEVIHGPL
CCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHHCCHHHEECCH
NDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATDCGIPEENCFIMDNGE
HHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCCCCCEEEEECCC
VLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVLRDRRILSEEGLVIVVVSIDMDDFKI
EEEEECCCCEEECCCCCCCEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEECCCCEEE
SAGPDLISRGFVYMRESGDLINDAQELISNHLQKVMERKTTQWSEIKNEITDTLAPFLYE
ECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEV
HHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377