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The map label for this gene is moaE

Identifier: 16078494

GI number: 16078494

Start: 1498966

End: 1499439

Strand: Direct

Name: moaE

Synonym: BSU14300

Alternate gene names: 16078494

Gene position: 1498966-1499439 (Clockwise)

Preceding gene: 16078493

Following gene: 16078495

Centisome position: 35.56

GC content: 46.2

Gene sequence:

>474_bases
ATGGAACGGTTTGAGATTACGAAAACACCCATTAATACTGAAAACATCATCAAAAAAGTGGAGAAACGGGAAGCGGGAGC
CATTACAACATTTATTGGTACGGTTCGGGAATGGACAAACGGAAAAAGAACGGTTCGGCTTGAATATGAGGCATATGAAC
CGATGGCCGTGCAAATGCTTGCTCAAATCGGCGCTGAAATCGAAGAAAAATGGGAAGGTGCCTCAGCTGCGATCACGCAC
CGTATAGGTGTGCTCGATATCGGTGAAGCAGCGGTAGTGATTGCTGTATCTTCTCCCCATCGAAAAGCCGCATATGAAGC
CAATGAGTATGCGATTGAACGCATTAAACAGATTGTGCCGATTTGGAAAAAAGAAATTTGGGAAGACGGTGAGCAATGGA
TCGGCGACCAGCTAGAAACGACAGCTTATCCGAACGGAAAACCAGACCTAAGCGAGGGAGAGCAGCATGATTAA

Upstream 100 bases:

>100_bases
AAGAGCATATGACAGAGCATCAGGGATTACCCGTTTTTCATGCGGATGATCCGGTTGCCGTGGATTTTGTGCTTTCACAG
CTGAAGGGGGAATCTGCATA

Downstream 100 bases:

>100_bases
AATTCTTTTATTTGCAGGGCTTGCTGAACAGGCCGGTACACAAGCAATAGAAATAGATATGGAACAAGCAACCACAGATG
AAATAAAAGCGAGTCTAAAA

Product: molybdopterin synthase large subunit

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQMLAQIGAEIEEKWEGASAAITH
RIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVPIWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD

Sequences:

>Translated_157_residues
MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQMLAQIGAEIEEKWEGASAAITH
RIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVPIWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD
>Mature_157_residues
MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQMLAQIGAEIEEKWEGASAAITH
RIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVPIWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family

Homologues:

Organism=Homo sapiens, GI4758732, Length=113, Percent_Identity=46.9026548672566, Blast_Score=116, Evalue=9e-27,
Organism=Escherichia coli, GI1787003, Length=114, Percent_Identity=35.0877192982456, Blast_Score=78, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17509279, Length=131, Percent_Identity=36.6412213740458, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356259, Length=132, Percent_Identity=39.3939393939394, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAE_BACSU (O31705)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   B69659
- RefSeq:   NP_389313.1
- ProteinModelPortal:   O31705
- SMR:   O31705
- EnsemblBacteria:   EBBACT00000002391
- GeneID:   936092
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14300
- NMPDR:   fig|224308.1.peg.1432
- GenoList:   BSU14300
- GeneTree:   EBGT00050000001665
- HOGENOM:   HBG460564
- OMA:   KARAPIW
- PhylomeDB:   O31705
- ProtClustDB:   CLSK887227
- BioCyc:   BSUB:BSU14300-MONOMER
- InterPro:   IPR003448
- Gene3D:   G3DSA:3.90.1170.40
- PANTHER:   PTHR23404:SF2

Pfam domain/function: PF02391 MoaE; SSF54690 Mb_biosynth_MoaE

EC number: NA

Molecular weight: Translated: 17782; Mature: 17782

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQML
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
AQIGAEIEEKWEGASAAITHRIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVP
HHHHHHHHHHHCCCHHHHHHHCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
IWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD
HHHHHHHHCHHHHHCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQML
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
AQIGAEIEEKWEGASAAITHRIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVP
HHHHHHHHHHHCCCHHHHHHHCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
IWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD
HHHHHHHHCHHHHHCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377