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The map label for this gene is moeA

Identifier: 16078492

GI number: 16078492

Start: 1497192

End: 1498484

Strand: Direct

Name: moeA

Synonym: BSU14280

Alternate gene names: 16078492

Gene position: 1497192-1498484 (Clockwise)

Preceding gene: 16078491

Following gene: 16078493

Centisome position: 35.52

GC content: 49.57

Gene sequence:

>1293_bases
ATGCTGGAGAAAAGAACACCGATACCGGTAGACGAGGCAGTTCGGCGTGTCTGCCATTTTCAAAAACAAGGTGAAACAGA
ATGGGTGGCGCTTGAAGACAGCCTTCATCGGTTTTTAGCCGAAGATATAACTGCGGATCACCACGTACCTGCGTTTGACC
GTTCGCCATACGATGGTTTTGCTGTCAGAGCGTGCGATACGGCAGAAGCTTCACGTGAAAACCCAGTTCGGTTTGAAGTT
ATTGACCATATTGGAGCGGGAGCAGTATCTGAAAAAGAACTTGGCCCGTTTCAGGCCGTGCGGATTATGACTGGCGCGCA
AATCCCTGAAGGGGCAGATGCAGTCGTCATGATTGAACTGACTCAAGCCTTTGAGGAGAATGGGAAGTCATTTATGTCTT
TAAAACGCCGCTTTCAGACGGGGGATAATATTTCAAAAACAGGTGAGGACGCTCAAAAAGGGAGTGTTTTACTGACAAAG
GGCACCCGGGTGACACCAGGAGTGACGGCTCTTTTGGCAACATTCGGATACGCATCTGTGCCTGTCGTGAGAAAGCCTGT
AGTCGGGATTATCGCAACCGGTACCGAATTGCTGAATGTCAGTGATCCGCTGGAACCGGGAAAAATCCGCAACAGCAATG
CGAGTATGGTATATGCGCAAGCGATAGAAGCAGGCGCAACACCGCTTTATTTAGGGAAAATCTCTGATGAACTGGATAAA
AGCTTTGCTGCAGTTAAAGAAGCGATGAAAAAGGTGGACTTCCTGATCACAACTGGCGGTGTGTCAGTCGGTGACTTTGA
CTTTTTGCCTGCGATCTATGAAAAACTCGGAGCGGACGTGCTGTTTAATAAAGTTGCCATGCGCCCGGGCAGCGTGACAA
CGGTGGCGCATGCAAACGATATGCTGCTGTTTGGCCTTTCAGGTAATCCGTCGGCATGCTATGTCGGTTTTGAACTGTTT
GTCAAACCGATAATCCAGACATGGCTGCTCAATGAAACACCGCATTCAATTTGTGCGGAAGCTGTTTTGACCAAGGATTT
TCCGAAGCCGAACCCATTTACCCGATTTGTTCGTGCGCTCGTGCACCATCAGGAAGGGAAGCTGTTGGCGACACCCGTTG
GCTTAGATAAGTCGAGCTCTGTCACATCACTTGCAGATGCCAATGCATTTATTATCCTGCCGGGCGGTACGCGAGGCTAC
GAATCTGGCAGAACGGTTCAAGTGCTGCTCATCAGAGAAGAGAATGGAAGTGAATGGCCTTGGTCCGTCCTTTCCCGATC
GTCCAAGTTGTAG

Upstream 100 bases:

>100_bases
TCAATGACGGACGGGCACTGGTGCATGGAACAAATGATGTGAAAGAAGCCAACTCCGTGTTGGCAAGAGTGATTGGACTG
TAACAACAGGAGGGGTCAGA

Downstream 100 bases:

>100_bases
GATTTCAAAACAGCGGGAAAACAACGTTTATTGAGCGCATTCTTGAAAAAGCCTCTGAACAGGGAGTCCATTTGGGCTGC
CTGAAGCACCATGGTCACGG

Product: molybdene to molybdopterin ligation enzyme

Products: NA

Alternate protein names: MPT Mo-transferase

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MLEKRTPIPVDEAVRRVCHFQKQGETEWVALEDSLHRFLAEDITADHHVPAFDRSPYDGFAVRACDTAEASRENPVRFEV
IDHIGAGAVSEKELGPFQAVRIMTGAQIPEGADAVVMIELTQAFEENGKSFMSLKRRFQTGDNISKTGEDAQKGSVLLTK
GTRVTPGVTALLATFGYASVPVVRKPVVGIIATGTELLNVSDPLEPGKIRNSNASMVYAQAIEAGATPLYLGKISDELDK
SFAAVKEAMKKVDFLITTGGVSVGDFDFLPAIYEKLGADVLFNKVAMRPGSVTTVAHANDMLLFGLSGNPSACYVGFELF
VKPIIQTWLLNETPHSICAEAVLTKDFPKPNPFTRFVRALVHHQEGKLLATPVGLDKSSSVTSLADANAFIILPGGTRGY
ESGRTVQVLLIREENGSEWPWSVLSRSSKL

Sequences:

>Translated_430_residues
MLEKRTPIPVDEAVRRVCHFQKQGETEWVALEDSLHRFLAEDITADHHVPAFDRSPYDGFAVRACDTAEASRENPVRFEV
IDHIGAGAVSEKELGPFQAVRIMTGAQIPEGADAVVMIELTQAFEENGKSFMSLKRRFQTGDNISKTGEDAQKGSVLLTK
GTRVTPGVTALLATFGYASVPVVRKPVVGIIATGTELLNVSDPLEPGKIRNSNASMVYAQAIEAGATPLYLGKISDELDK
SFAAVKEAMKKVDFLITTGGVSVGDFDFLPAIYEKLGADVLFNKVAMRPGSVTTVAHANDMLLFGLSGNPSACYVGFELF
VKPIIQTWLLNETPHSICAEAVLTKDFPKPNPFTRFVRALVHHQEGKLLATPVGLDKSSSVTSLADANAFIILPGGTRGY
ESGRTVQVLLIREENGSEWPWSVLSRSSKL
>Mature_430_residues
MLEKRTPIPVDEAVRRVCHFQKQGETEWVALEDSLHRFLAEDITADHHVPAFDRSPYDGFAVRACDTAEASRENPVRFEV
IDHIGAGAVSEKELGPFQAVRIMTGAQIPEGADAVVMIELTQAFEENGKSFMSLKRRFQTGDNISKTGEDAQKGSVLLTK
GTRVTPGVTALLATFGYASVPVVRKPVVGIIATGTELLNVSDPLEPGKIRNSNASMVYAQAIEAGATPLYLGKISDELDK
SFAAVKEAMKKVDFLITTGGVSVGDFDFLPAIYEKLGADVLFNKVAMRPGSVTTVAHANDMLLFGLSGNPSACYVGFELF
VKPIIQTWLLNETPHSICAEAVLTKDFPKPNPFTRFVRALVHHQEGKLLATPVGLDKSSSVTSLADANAFIILPGGTRGY
ESGRTVQVLLIREENGSEWPWSVLSRSSKL

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family

Homologues:

Organism=Homo sapiens, GI66932975, Length=400, Percent_Identity=35.5, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI10880983, Length=400, Percent_Identity=35.5, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1787049, Length=388, Percent_Identity=33.5051546391753, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI25147659, Length=388, Percent_Identity=29.3814432989691, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17562606, Length=350, Percent_Identity=28.2857142857143, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17136978, Length=378, Percent_Identity=30.4232804232804, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24638841, Length=378, Percent_Identity=30.4232804232804, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOEA_BACSU (O31703)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   E69659
- RefSeq:   NP_389311.1
- HSSP:   P12281
- ProteinModelPortal:   O31703
- SMR:   O31703
- EnsemblBacteria:   EBBACT00000001091
- GeneID:   938797
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14280
- NMPDR:   fig|224308.1.peg.1430
- GenoList:   BSU14280
- GeneTree:   EBGT00050000000660
- HOGENOM:   HBG731522
- OMA:   TGSLMSE
- ProtClustDB:   CLSK2518227
- BioCyc:   BSUB:BSU14280-MONOMER
- InterPro:   IPR020817
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453
- Gene3D:   G3DSA:3.40.980.10
- SMART:   SM00852
- TIGRFAMs:   TIGR00177

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N; SSF53218 MoCF_biosynth; SSF63867 MoeA_C; SSF63882 MoeA_N

EC number: NA

Molecular weight: Translated: 46620; Mature: 46620

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKRTPIPVDEAVRRVCHFQKQGETEWVALEDSLHRFLAEDITADHHVPAFDRSPYDGF
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
AVRACDTAEASRENPVRFEVIDHIGAGAVSEKELGPFQAVRIMTGAQIPEGADAVVMIEL
EEEEECCHHCCCCCCEEEEEEHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEE
TQAFEENGKSFMSLKRRFQTGDNISKTGEDAQKGSVLLTKGTRVTPGVTALLATFGYASV
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHCCCEEEECCCCCCCHHHHHHHHHCCCCC
PVVRKPVVGIIATGTELLNVSDPLEPGKIRNSNASMVYAQAIEAGATPLYLGKISDELDK
CHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCEEEEECHHHHHHH
SFAAVKEAMKKVDFLITTGGVSVGDFDFLPAIYEKLGADVLFNKVAMRPGSVTTVAHAND
HHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCC
MLLFGLSGNPSACYVGFELFVKPIIQTWLLNETPHSICAEAVLTKDFPKPNPFTRFVRAL
EEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
VHHQEGKLLATPVGLDKSSSVTSLADANAFIILPGGTRGYESGRTVQVLLIREENGSEWP
HHCCCCCEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCC
WSVLSRSSKL
HHHHCCCCCC
>Mature Secondary Structure
MLEKRTPIPVDEAVRRVCHFQKQGETEWVALEDSLHRFLAEDITADHHVPAFDRSPYDGF
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
AVRACDTAEASRENPVRFEVIDHIGAGAVSEKELGPFQAVRIMTGAQIPEGADAVVMIEL
EEEEECCHHCCCCCCEEEEEEHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEE
TQAFEENGKSFMSLKRRFQTGDNISKTGEDAQKGSVLLTKGTRVTPGVTALLATFGYASV
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHCCCEEEECCCCCCCHHHHHHHHHCCCCC
PVVRKPVVGIIATGTELLNVSDPLEPGKIRNSNASMVYAQAIEAGATPLYLGKISDELDK
CHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCEEEEECHHHHHHH
SFAAVKEAMKKVDFLITTGGVSVGDFDFLPAIYEKLGADVLFNKVAMRPGSVTTVAHAND
HHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCC
MLLFGLSGNPSACYVGFELFVKPIIQTWLLNETPHSICAEAVLTKDFPKPNPFTRFVRAL
EEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
VHHQEGKLLATPVGLDKSSSVTSLADANAFIILPGGTRGYESGRTVQVLLIREENGSEWP
HHCCCCCEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCC
WSVLSRSSKL
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377