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The map label for this gene is moeB

Identifier: 16078491

GI number: 16078491

Start: 1496155

End: 1497174

Strand: Direct

Name: moeB

Synonym: BSU14270

Alternate gene names: 16078491

Gene position: 1496155-1497174 (Clockwise)

Preceding gene: 16078490

Following gene: 16078492

Centisome position: 35.49

GC content: 47.84

Gene sequence:

>1020_bases
ATGGAGGAACGTTATTCACGGCAAATCAGATTTAAGCAAATTGGGGAAGAGGGCCAGAAAAGACTGGCAGACAGCCATGT
GCTGATTGTCGGAGCGGGAGCGCTCGGCACTGCAGGAGCTGAAGGGCTTTCGAGAGCGGGAGTCGGTACGATTACCATTA
TTGACCGTGACTATGTGGAGTGGAGCAATCTTCAAAGGCAGCAGCTTTATACAGAAAGCGATGCCAAACTCCGCATGCCG
AAAGCCATGGCTGCCAAAGAGCATCTATCAGCGATTAACAGCGAGATACATATTGAGGCGTATGTTACCGAAGGAACAGC
CGAAACGCTTGAGCCGCTGATTGAAAAAGCAGATGTCGTGATCGACGCGACAGATAATTTTGAAACCCGCATGCTGATAA
ACGATCTTGCCCAGAAAACAAAGACACCTTGGGTCTATGGCGCCTGTGTAAGCAGCCAGGGAATGTTCATGACTGTCATT
CCCGGAGAAACCCCTTGTCTGTCTTGTTTGTTTGAGCAAATTCCTGTCGGCGGGGCGACGTGTGATACTGCCGGCATTAT
TCCGCCTGCTGTGCATATCGTTTCAGCATATCAGCAGGCTGAGGCATTAAAGCTGTTAACCGGCCAAAAAGAAGCAATAC
AGCGGGGGTTTGTGACATTTGATGTCTGGAACAATTCGCATATGAAGATTAACGTTAATCATGTACGCCGTGAGGAATGC
CCATCTTGCGGAGCTAATGCGGTATATCCTTATCTTCAGGATTGGAACACACCAAAAGCTGCTGTACTATGCGGCCGTGA
TACAGTGCAGGTAAGGTCAGAATCATTAAAAAGAATCCCGAAACAGGAGCTCATTAAGCGGCTGAAAACAATCGGCAAGG
TCGAGGCTAATGCGTTTTTGCTGCATATTTTTTACGAGGATTTCAGAATTGTCATTTTCAATGACGGACGGGCACTGGTG
CATGGAACAAATGATGTGAAAGAAGCCAACTCCGTGTTGGCAAGAGTGATTGGACTGTAA

Upstream 100 bases:

>100_bases
ATGATTTTAGCTGTTTGGAAGAAAAATCGAACTCCCTCAAGCGGGACTGACGGGGCAATAACATGATAAGATGACATAAA
AGATAAGGAGAACAGGTTTG

Downstream 100 bases:

>100_bases
CAACAGGAGGGGTCAGAATGCTGGAGAAAAGAACACCGATACCGGTAGACGAGGCAGTTCGGCGTGTCTGCCATTTTCAA
AAACAAGGTGAAACAGAATG

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWSNLQRQQLYTESDAKLRMP
KAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVIDATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVI
PGETPCLSCLFEQIPVGGATCDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREEC
PSCGANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTIGKVEANAFLLHIFYEDFRIVIFNDGRALV
HGTNDVKEANSVLARVIGL

Sequences:

>Translated_339_residues
MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWSNLQRQQLYTESDAKLRMP
KAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVIDATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVI
PGETPCLSCLFEQIPVGGATCDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREEC
PSCGANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTIGKVEANAFLLHIFYEDFRIVIFNDGRALV
HGTNDVKEANSVLARVIGL
>Mature_339_residues
MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWSNLQRQQLYTESDAKLRMP
KAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVIDATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVI
PGETPCLSCLFEQIPVGGATCDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREEC
PSCGANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTIGKVEANAFLLHIFYEDFRIVIFNDGRALV
HGTNDVKEANSVLARVIGL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family

Homologues:

Organism=Homo sapiens, GI7657339, Length=300, Percent_Identity=30.3333333333333, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI150417996, Length=158, Percent_Identity=29.746835443038, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=35.8024691358025, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=36.5461847389558, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17540406, Length=246, Percent_Identity=32.1138211382114, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321903, Length=251, Percent_Identity=29.0836653386454, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320598, Length=183, Percent_Identity=27.3224043715847, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325323, Length=162, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24582879, Length=254, Percent_Identity=30.7086614173228, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28573937, Length=173, Percent_Identity=32.3699421965318, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOEB_BACSU (O31702)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   F69659
- RefSeq:   NP_389310.1
- HSSP:   P12282
- ProteinModelPortal:   O31702
- SMR:   O31702
- EnsemblBacteria:   EBBACT00000001237
- GeneID:   938780
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14270
- NMPDR:   fig|224308.1.peg.1429
- GenoList:   BSU14270
- GeneTree:   EBGT00070000032023
- HOGENOM:   HBG535305
- OMA:   LLHGTEN
- ProtClustDB:   PRK12475
- BioCyc:   BSUB:BSU14270-MONOMER
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF; SSF69572 MoeB

EC number: NA

Molecular weight: Translated: 37517; Mature: 37517

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 34-34 BINDING 55-55 BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVE
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCEEEEEEECCCHH
WSNLQRQQLYTESDAKLRMPKAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVV
HHCCHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCEE
IDATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVIPGETPCLSCLFEQIPVGGAT
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCC
CDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREEC
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCEEEEEHHHHHHHCC
PSCGANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTIGKVEANAFL
CCCCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEE
LHIFYEDFRIVIFNDGRALVHGTNDVKEANSVLARVIGL
EEEEECCEEEEEEECCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVE
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCEEEEEEECCCHH
WSNLQRQQLYTESDAKLRMPKAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVV
HHCCHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCEE
IDATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVIPGETPCLSCLFEQIPVGGAT
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCC
CDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREEC
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCEEEEEHHHHHHHCC
PSCGANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTIGKVEANAFL
CCCCCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEE
LHIFYEDFRIVIFNDGRALVHGTNDVKEANSVLARVIGL
EEEEECCEEEEEEECCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377