BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is ykuF

Identifier: 16078470

GI number: 16078470

Start: 1478072

End: 1478836

Strand: Direct

Name: ykuF

Synonym: BSU14060

Alternate gene names: 16078470

Gene position: 1478072-1478836 (Clockwise)

Preceding gene: 16078465

Following gene: 255767329

Centisome position: 35.06

GC content: 50.85

Gene sequence:

>765_bases
ATGGAAAAAAAGGCGGTAATTATTACCGGCGGGTCAAGCGGCATGGGAAAAGCGATGGCAAAAAAACAGGCTGAATTAGG
GTGGCACGTTATGGTGACAGGGAGGAACCATGAGGCACTTGAGGAAACAAAAAAAGAAATTCAGACCTTTGAGGGACAGG
TCGCTTGTTTTCAAATGGATGTCCGTTCCGATTCCGCCGCTTCAGACATGATTAAAGAAGCGGTAAAAGCCTTTGGCCGG
CTTGACGCGCTGATTAACAACGCGGCCGGAAACTTTATTTGTCCGGCAGAAAAGCTGACGCCCAATGGATGGAAAGCCGT
TATTGAGATTGTTTTAAACGGCACATTTTTTTGCAGCCAAGCAGCGGCAAGGCATTGGATTGACCAGAAGCAGCAGGGTG
TCATTTTAAATATGGCAGCCACGTACGCTTGGGGAGCGGGAGCGGGGGTCGTTCATTCCGCTGCAGCCAAAGCGGGGGTA
TTGTCATTGACAAGAACGCTGGCCGTTGAATGGGGGAGCAAATACGGCATTCGTACAAACGCGATAGCCCCCGGACCGAT
TGAACGAACAGGCGGTGCGGAGAAGCTATTTGAATCGGAAAAAGCGATGGCCCGCACGATGAACAGTGTGCCGCTCGGCC
GATTGGGCACACCGGAGGAAATCGCCGCTTTGGCAGCGTTTTTGCTTTCTGATGAAGCATCCTATATAAACGGGGATTGT
ATCACAATGGACGGGGGGCAATGGCTGAATCCTTATCCGTTTTAA

Upstream 100 bases:

>100_bases
TATCTGATAAAAATCTCATATGAGCATGTGCTGACAGAGGCAGGGATATAATGATAGAATATTATTGAATGAATAATCAT
TCACAATTGGAGGGATAAAC

Downstream 100 bases:

>100_bases
TCTGAATATTTATAAGTGAATATTTAAGTTATTAAGAACTTTTATGTTCGAATGTAATACCTCCACAATTAACCTTCTTC
CTATCCAATTTGATAACACA

Product: short chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQTFEGQVACFQMDVRSDSAASDMIKEAVKAFGR
LDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQAAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGV
LSLTRTLAVEWGSKYGIRTNAIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDC
ITMDGGQWLNPYPF

Sequences:

>Translated_254_residues
MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQTFEGQVACFQMDVRSDSAASDMIKEAVKAFGR
LDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQAAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGV
LSLTRTLAVEWGSKYGIRTNAIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDC
ITMDGGQWLNPYPF
>Mature_254_residues
MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQTFEGQVACFQMDVRSDSAASDMIKEAVKAFGR
LDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQAAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGV
LSLTRTLAVEWGSKYGIRTNAIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDC
ITMDGGQWLNPYPF

Specific function: Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3- enoyl-CoA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily

Homologues:

Organism=Homo sapiens, GI4503301, Length=225, Percent_Identity=41.3333333333333, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=34.2307692307692, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI10190704, Length=251, Percent_Identity=37.8486055776892, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI15277342, Length=254, Percent_Identity=30.7086614173228, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI32483357, Length=253, Percent_Identity=28.4584980237154, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=30.4878048780488, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI59889578, Length=195, Percent_Identity=32.8205128205128, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI7705925, Length=246, Percent_Identity=26.0162601626016, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI5031737, Length=253, Percent_Identity=26.4822134387352, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4504505, Length=193, Percent_Identity=29.5336787564767, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI126723750, Length=257, Percent_Identity=25.6809338521401, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4758504, Length=263, Percent_Identity=28.8973384030418, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI66933014, Length=255, Percent_Identity=28.6274509803922, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31542939, Length=189, Percent_Identity=29.1005291005291, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=32.9317269076305, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787335, Length=249, Percent_Identity=32.9317269076305, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=28.8, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=30.46875, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=31.7460317460317, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87082160, Length=249, Percent_Identity=28.9156626506024, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790717, Length=243, Percent_Identity=27.1604938271605, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=30, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI2367365, Length=243, Percent_Identity=28.8065843621399, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=27.9527559055118, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787820, Length=242, Percent_Identity=26.0330578512397, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787526, Length=248, Percent_Identity=24.5967741935484, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787545, Length=263, Percent_Identity=26.2357414448669, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=251, Percent_Identity=42.2310756972112, Blast_Score=182, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17536651, Length=248, Percent_Identity=41.1290322580645, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17561272, Length=235, Percent_Identity=45.1063829787234, Blast_Score=174, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=32.0610687022901, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17562908, Length=261, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17562906, Length=261, Percent_Identity=29.5019157088123, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=34.3283582089552, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=32.6996197718631, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531453, Length=264, Percent_Identity=32.9545454545455, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=34.3396226415094, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=31.9702602230483, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=33.1983805668016, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=31.1787072243346, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=30.9701492537313, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=32.2097378277154, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=29.296875, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17563726, Length=273, Percent_Identity=34.4322344322344, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=31.1787072243346, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25147288, Length=241, Percent_Identity=30.7053941908714, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=28.4584980237154, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=258, Percent_Identity=29.0697674418605, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=263, Percent_Identity=29.277566539924, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=243, Percent_Identity=25.9259259259259, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=270, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=26.9662921348315, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=264, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=195, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=251, Percent_Identity=27.8884462151394, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=254, Percent_Identity=26.7716535433071, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=193, Percent_Identity=27.979274611399, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=34.136546184739, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322861, Length=277, Percent_Identity=29.6028880866426, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639444, Length=248, Percent_Identity=34.6774193548387, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI23397609, Length=260, Percent_Identity=31.5384615384615, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21355319, Length=251, Percent_Identity=27.8884462151394, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21357041, Length=261, Percent_Identity=30.6513409961686, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24644339, Length=248, Percent_Identity=30.6451612903226, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=29.5275590551181, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24643142, Length=244, Percent_Identity=26.6393442622951, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17737361, Length=271, Percent_Identity=29.8892988929889, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=29.2307692307692, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644337, Length=269, Percent_Identity=29.7397769516729, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADH_BACSU (O34717)

Other databases:

- EMBL:   AJ222587
- EMBL:   AL009126
- PIR:   C69865
- RefSeq:   NP_389289.1
- ProteinModelPortal:   O34717
- SMR:   O34717
- EnsemblBacteria:   EBBACT00000003315
- GeneID:   939226
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14060
- NMPDR:   fig|224308.1.peg.1408
- GenoList:   BSU14060
- GeneTree:   EBGT00070000031907
- HOGENOM:   HBG750976
- OMA:   HEALEET
- PhylomeDB:   O34717
- ProtClustDB:   PRK07677
- BioCyc:   BSUB:BSU14060-MONOMER
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: =1.3.1.34

Molecular weight: Translated: 27126; Mature: 27126

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 142-142 BINDING 100-100 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQTFEGQVACFQMD
CCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEE
VRSDSAASDMIKEAVKAFGRLDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHCCCCHHHHHHHHHHCCCHHHHH
AAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGVLSLTRTLAVEWGSKYGIRTN
HHHHHHHHHHHCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
AIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCE
ITMDGGQWLNPYPF
EEECCCCCCCCCCC
>Mature Secondary Structure
MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQTFEGQVACFQMD
CCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEE
VRSDSAASDMIKEAVKAFGRLDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHCCCCHHHHHHHHHHCCCHHHHH
AAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGVLSLTRTLAVEWGSKYGIRTN
HHHHHHHHHHHCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
AIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCE
ITMDGGQWLNPYPF
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377