Click here to switch to the map view.

The map label for this gene is clpE

Identifier: 16078434

GI number: 16078434

Start: 1435628

End: 1437727

Strand: Reverse

Name: clpE

Synonym: BSU13700

Alternate gene names: 16078434

Gene position: 1437727-1435628 (Counterclockwise)

Preceding gene: 16078440

Following gene: 16078433

Centisome position: 34.1

GC content: 44.9

Gene sequence:

>2100_bases
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGAAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTAAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCTCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCATGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAGGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCAAAATAA

Upstream 100 bases:

>100_bases
TTTGCCGTATACTAATAGTCAAAGAAGGTCAAACCCAATAGACCTTTTTATTCACTTTCATTGGTCAAAGATGATCAAAT
TATTAAGGAGGTTTTGGCAA

Downstream 100 bases:

>100_bases
CAATCAGCGGTTTCCTTTTAGGAAGCCGCTTTTTTTTATACTTTCATACTCTTGACTTAGAAATTACCGTCCCTTCTCCT
GAAGCGCTACTTCCCTTCAT

Product: ATP-dependent Clp protease (class III stress gene)

Products: NA

Alternate protein names: ATPase ClpE; Heat shock protein HSP1

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Sequences:

>Translated_699_residues
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK
>Mature_699_residues
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Specific function: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Homo sapiens, GI13540606, Length=300, Percent_Identity=35.6666666666667, Blast_Score=186, Evalue=6e-47,
Organism=Escherichia coli, GI1788943, Length=695, Percent_Identity=47.1942446043166, Blast_Score=600, Evalue=1e-172,
Organism=Escherichia coli, GI1787109, Length=661, Percent_Identity=41.3010590015129, Blast_Score=495, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320464, Length=733, Percent_Identity=42.4283765347885, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6323002, Length=333, Percent_Identity=40.8408408408408, Blast_Score=248, Evalue=2e-66,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPE_BACSU (O31673)

Other databases:

- EMBL:   AL009126
- PIR:   A69601
- RefSeq:   NP_389253.1
- HSSP:   P03815
- ProteinModelPortal:   O31673
- SMR:   O31673
- EnsemblBacteria:   EBBACT00000001230
- GeneID:   939289
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13700
- NMPDR:   fig|224308.1.peg.1372
- GenoList:   BSU13700
- GeneTree:   EBGT00050000000358
- HOGENOM:   HBG413133
- OMA:   KHAVSKI
- ProtClustDB:   CLSK887197
- BioCyc:   BSUB:BSU13700-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR001943
- PRINTS:   PR00300
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small; PF02151 UVR

EC number: NA

Molecular weight: Translated: 77907; Mature: 77907

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPK
CCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCH
EQSAAKQSEKKGLLDELAQNITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGE
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
PGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLITELK
CCCCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHH
ERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
HCCCEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDK
HHCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
AIDLLDEAGSKANLLIDELNDEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKK
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKMKELEAKLHERVIGQEAAVQK
CCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
VAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEECHHHHHH
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCC
TDSQGRTVSFKDTVIIMTSNAGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRF
CCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHH
DSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTDEAKEKIAELGYHPSFGARPL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHH
RRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEC
>Mature Secondary Structure
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPK
CCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCH
EQSAAKQSEKKGLLDELAQNITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGE
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
PGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLITELK
CCCCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHH
ERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
HCCCEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDK
HHCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
AIDLLDEAGSKANLLIDELNDEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKK
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKMKELEAKLHERVIGQEAAVQK
CCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
VAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEECHHHHHH
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCC
TDSQGRTVSFKDTVIIMTSNAGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRF
CCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHH
DSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTDEAKEKIAELGYHPSFGARPL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHH
RRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377