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The map label for this gene is kinD

Identifier: 16078430

GI number: 16078430

Start: 1431486

End: 1433006

Strand: Reverse

Name: kinD

Synonym: BSU13660

Alternate gene names: 16078430

Gene position: 1433006-1431486 (Counterclockwise)

Preceding gene: 16078432

Following gene: 16078427

Centisome position: 33.99

GC content: 41.55

Gene sequence:

>1521_bases
ATGTTGGAGCGATGCAAATTGAAAATACTAAAAGGCGCCTGCGGGAGAGTCAAACTTTATATCATACTGGTCGTGATTCC
GGCAATCGTCATCAGCTTTTTCGTATATGAAAAAGAAAAAGATACAATAGCTGCAGAACATAAACAAGAAGCAAGTGTCC
TGCTAAACCTTCACCGCAACAAAATCAATTATTTAATCGGAGAAACAATGGCGAGAATGACCTCCTTGTCCATCGCCATC
GACAGACCGGTTGATATAAAGAAAATGCAATCAATTTTGGAAAAGACCTTTGATTCAGAGCCGAGATTTTCCGGTCTCTA
TTTTCTCAATGCCAAAGGAGACGTCACCGCAAGCACAACCGAATTAAAAACGAAAGTAAACCTGGCCGACAGATCTTTTT
TTATAAAAGCAAAGGAAACTAAAAAGACAGTCATTTCAGATAGTTATTCGAGCAGAATTACTGGTCAGCCAATTTTCACA
ATTTGTGTGCCTGTTTTGGACTCAAAACGTAATGTGACAGATTATCTTGTCGCAGCTATTCAAATTGATTATTTGAAAAA
TCTCATTAACTTACTAAGCCCTGATGTTTATATTGAAGTTGTGAACCAAGACGGGAAAATGATTTTTGCGAGCGGACAAG
CCTCTCATGCAGAGGATCAGAAACCTGTCAGCGGATATCTGGATGATATCAGCTGGAATATGAAAGTCTATCCGAACCCG
GTCACAATAGAAGAACTGTCGAAAAGCCTTGTGCTTCCGCTTTCATGTATTATTGTTCTGCTGAATATCCTTTTTATTCT
CGTGCTGTATTATTTGCTGAAGCGGCAGACCCAGCTGGAGCGCTCAGAAAACGAGGCGCAAAAATTAGAGCTGATCGGGA
CGCTTGCTGCCAGCACAGCCCATGAAATCCGTAACCCCCTCACCGGGATAAGCGGCTTTATTCAGCTGTTGCAAAAGAAA
TATAAAGGTGAGGAAGATCAGCTTTACTTCTCCATTATCGAACAGGAGATAAAGCGCATCAATCAAATAGTGAGTGAGTT
TCTCGTTCTCGGCAAGCCGACAGCTGAAAAATGGGAGCTGAACTCACTTCAGGACATTATCGGAGAAATTATGCCGATCA
TTTATTCTGAGGGCAATCTATACAATGTTGAAGTCGAATTACAGTATCTAACCGAGCAGCCCTTACTCGTAAAATGTACA
AAAGATCATATTAAACAAGTGATTTTAAATGTAGCAAAAAATGGCCTCGAGTCAATGCCTGAAGGAGGCAAACTGACGAT
CTCCCTAGGAGCTTTAGATAAAAAAGCCATAATCAAAGTTGTGGATAACGGTGAAGGGATTTCTCAGGAAATGCTGGATC
ACATCTTCCTTCCCTTTGTTACTTCTAAAGAAAAAGGAACCGGTCTCGGCCTTGTTGTTTGTAAACGGATCGTGCTGATG
TACGGAGGCTCTATTCATATTGAAAGTGAAGTGCGAAGAGGCACAGAGGTGACGATCACCCTCCCCGTATCCGCATCATA
G

Upstream 100 bases:

>100_bases
TTTTAAAATGTAAGCTTGCCAACATCTTTTTCTAATTTTTTTGGTTATTTTTTCTTATTAAAGAAGAAGAAACAATGATA
TAATACCTCTAATCAGGTGT

Downstream 100 bases:

>100_bases
CCCCCCTGACCATGTCAGGAGGGTTTTTTATCTTCGTGATCGGGCATTGCCATTCGATTTGCTTGCTGGTCTTCCAGAAA
AAAGGATTTTGATGAAACGC

Product: histidine kinase phosphorylating Spo0A

Products: NA

Alternate protein names: Sensor histidine kinase D

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLERCKLKILKGACGRVKLYIILVVIPAIVISFFVYEKEKDTIAAEHKQEASVLLNLHRNKINYLIGETMARMTSLSIAI
DRPVDIKKMQSILEKTFDSEPRFSGLYFLNAKGDVTASTTELKTKVNLADRSFFIKAKETKKTVISDSYSSRITGQPIFT
ICVPVLDSKRNVTDYLVAAIQIDYLKNLINLLSPDVYIEVVNQDGKMIFASGQASHAEDQKPVSGYLDDISWNMKVYPNP
VTIEELSKSLVLPLSCIIVLLNILFILVLYYLLKRQTQLERSENEAQKLELIGTLAASTAHEIRNPLTGISGFIQLLQKK
YKGEEDQLYFSIIEQEIKRINQIVSEFLVLGKPTAEKWELNSLQDIIGEIMPIIYSEGNLYNVEVELQYLTEQPLLVKCT
KDHIKQVILNVAKNGLESMPEGGKLTISLGALDKKAIIKVVDNGEGISQEMLDHIFLPFVTSKEKGTGLGLVVCKRIVLM
YGGSIHIESEVRRGTEVTITLPVSAS

Sequences:

>Translated_506_residues
MLERCKLKILKGACGRVKLYIILVVIPAIVISFFVYEKEKDTIAAEHKQEASVLLNLHRNKINYLIGETMARMTSLSIAI
DRPVDIKKMQSILEKTFDSEPRFSGLYFLNAKGDVTASTTELKTKVNLADRSFFIKAKETKKTVISDSYSSRITGQPIFT
ICVPVLDSKRNVTDYLVAAIQIDYLKNLINLLSPDVYIEVVNQDGKMIFASGQASHAEDQKPVSGYLDDISWNMKVYPNP
VTIEELSKSLVLPLSCIIVLLNILFILVLYYLLKRQTQLERSENEAQKLELIGTLAASTAHEIRNPLTGISGFIQLLQKK
YKGEEDQLYFSIIEQEIKRINQIVSEFLVLGKPTAEKWELNSLQDIIGEIMPIIYSEGNLYNVEVELQYLTEQPLLVKCT
KDHIKQVILNVAKNGLESMPEGGKLTISLGALDKKAIIKVVDNGEGISQEMLDHIFLPFVTSKEKGTGLGLVVCKRIVLM
YGGSIHIESEVRRGTEVTITLPVSAS
>Mature_506_residues
MLERCKLKILKGACGRVKLYIILVVIPAIVISFFVYEKEKDTIAAEHKQEASVLLNLHRNKINYLIGETMARMTSLSIAI
DRPVDIKKMQSILEKTFDSEPRFSGLYFLNAKGDVTASTTELKTKVNLADRSFFIKAKETKKTVISDSYSSRITGQPIFT
ICVPVLDSKRNVTDYLVAAIQIDYLKNLINLLSPDVYIEVVNQDGKMIFASGQASHAEDQKPVSGYLDDISWNMKVYPNP
VTIEELSKSLVLPLSCIIVLLNILFILVLYYLLKRQTQLERSENEAQKLELIGTLAASTAHEIRNPLTGISGFIQLLQKK
YKGEEDQLYFSIIEQEIKRINQIVSEFLVLGKPTAEKWELNSLQDIIGEIMPIIYSEGNLYNVEVELQYLTEQPLLVKCT
KDHIKQVILNVAKNGLESMPEGGKLTISLGALDKKAIIKVVDNGEGISQEMLDHIFLPFVTSKEKGTGLGLVVCKRIVLM
YGGSIHIESEVRRGTEVTITLPVSAS

Specific function: Phosphorylates the sporulation-regulatory protein spo0F and, to a minor extent, is responsible for heterogeneous expression of spo0A during logarithmical growth. Also phosphorylates spo0A under biofilm growth conditions

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1790436, Length=264, Percent_Identity=32.1969696969697, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=30.7377049180328, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=30.1255230125523, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=29.3388429752066, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=23.0188679245283, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=222, Percent_Identity=27.4774774774775, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=28.5087719298246, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=223, Percent_Identity=25.1121076233184, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=24.5833333333333, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=221, Percent_Identity=23.9819004524887, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KIND_BACSU (O31671)

Other databases:

- EMBL:   AL009126
- PIR:   F69867
- RefSeq:   NP_389249.1
- PDB:   3FOS
- PDBsum:   3FOS
- ProteinModelPortal:   O31671
- EnsemblBacteria:   EBBACT00000000813
- GeneID:   939300
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13660
- NMPDR:   fig|224308.1.peg.1368
- GenoList:   BSU13660
- GeneTree:   EBGT00070000031947
- HOGENOM:   HBG313683
- OMA:   NNETHAQ
- ProtClustDB:   CLSK887195
- BioCyc:   BSUB:BSU13660-MONOMER
- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 56705; Mature: 56705

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb50b0d8)-; HASH(0xe19bbd0)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERCKLKILKGACGRVKLYIILVVIPAIVISFFVYEKEKDTIAAEHKQEASVLLNLHRN
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KINYLIGETMARMTSLSIAIDRPVDIKKMQSILEKTFDSEPRFSGLYFLNAKGDVTASTT
HHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECHH
ELKTKVNLADRSFFIKAKETKKTVISDSYSSRITGQPIFTICVPVLDSKRNVTDYLVAAI
HHEEEEEECCCEEEEEECHHHHHHHCCCCCCCCCCCCHHEEEEEHHCCCCCHHHHHHHHH
QIDYLKNLINLLSPDVYIEVVNQDGKMIFASGQASHAEDQKPVSGYLDDISWNMKVYPNP
HHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCC
VTIEELSKSLVLPLSCIIVLLNILFILVLYYLLKRQTQLERSENEAQKLELIGTLAASTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEIRNPLTGISGFIQLLQKKYKGEEDQLYFSIIEQEIKRINQIVSEFLVLGKPTAEKWEL
HHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
NSLQDIIGEIMPIIYSEGNLYNVEVELQYLTEQPLLVKCTKDHIKQVILNVAKNGLESMP
HHHHHHHHHHHHHEECCCCEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCC
EGGKLTISLGALDKKAIIKVVDNGEGISQEMLDHIFLPFVTSKEKGTGLGLVVCKRIVLM
CCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHH
YGGSIHIESEVRRGTEVTITLPVSAS
HCCCEEEEHHHCCCCEEEEEEECCCH
>Mature Secondary Structure
MLERCKLKILKGACGRVKLYIILVVIPAIVISFFVYEKEKDTIAAEHKQEASVLLNLHRN
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KINYLIGETMARMTSLSIAIDRPVDIKKMQSILEKTFDSEPRFSGLYFLNAKGDVTASTT
HHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECHH
ELKTKVNLADRSFFIKAKETKKTVISDSYSSRITGQPIFTICVPVLDSKRNVTDYLVAAI
HHEEEEEECCCEEEEEECHHHHHHHCCCCCCCCCCCCHHEEEEEHHCCCCCHHHHHHHHH
QIDYLKNLINLLSPDVYIEVVNQDGKMIFASGQASHAEDQKPVSGYLDDISWNMKVYPNP
HHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCC
VTIEELSKSLVLPLSCIIVLLNILFILVLYYLLKRQTQLERSENEAQKLELIGTLAASTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEIRNPLTGISGFIQLLQKKYKGEEDQLYFSIIEQEIKRINQIVSEFLVLGKPTAEKWEL
HHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
NSLQDIIGEIMPIIYSEGNLYNVEVELQYLTEQPLLVKCTKDHIKQVILNVAKNGLESMP
HHHHHHHHHHHHHEECCCCEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCC
EGGKLTISLGALDKKAIIKVVDNGEGISQEMLDHIFLPFVTSKEKGTGLGLVVCKRIVLM
CCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHH
YGGSIHIESEVRRGTEVTITLPVSAS
HCCCEEEEHHHCCCCEEEEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377