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The map label for this gene is mtnA

Identifier: 16078419

GI number: 16078419

Start: 1422172

End: 1423233

Strand: Reverse

Name: mtnA

Synonym: BSU13550

Alternate gene names: 16078419

Gene position: 1423233-1422172 (Counterclockwise)

Preceding gene: 16078420

Following gene: 16078416

Centisome position: 33.76

GC content: 46.89

Gene sequence:

>1062_bases
ATGACCCATTCATTTGCTGTTCCACGTTCTGTTGAATGGAAAGAAACAGCTATTACCATTTTAAATCAGCAAAAGCTTCC
TGATGAGACGGAATATTTGGAGCTGACGACGAAAGAGGATGTATTTGATGCGATTGTCACGCTCAAAGTAAGGGGCGCTC
CGGCGATCGGCATTACAGCTGCCTTCGGACTGGCGCTTGCTGCCAAAGACATTGAAACAGACAATGTCACGGAATTCCGC
CGCAGACTGGAAGACATCAAACAATATTTAAACAGCTCACGGCCTACCGCCATTAATTTGTCATGGGCGCTTGAGAGACT
GAGTCATTCGGTCGAAAACGCGATTTCCGTAAATGAAGCAAAAACAAACCTTGTCCATGAAGCCATTCAGATTCAGGTCG
AGGATGAGGAAACGTGCCGGTTGATCGGCCAAAACGCCCTGCAGCTTTTCAAAAAAGGCGACAGGATCATGACGATCTGC
AATGCCGGCTCAATCGCGACAAGCCGCTATGGAACGGCGCTAGCTCCGTTTTACTTGGCCAAACAAAAAGATTTGGGATT
ACACATTTATGCCTGTGAAACAAGGCCTGTCCTGCAAGGTTCACGCCTTACTGCTTGGGAGCTGATGCAGGGGGGCATTG
ATGTCACTCTGATCACAGACAGCATGGCCGCCCATACGATGAAGGAAAAACAAATTTCCGCTGTTATCGTCGGAGCCGAC
CGAATTGCAAAGAACGGTGATACGGCAAATAAAATCGGCACATACGGTCTTGCTATTTTAGCAAACGCCTTTGATATTCC
GTTTTTTGTCGCCGCACCTTTATCTACATTTGATACAAAGGTCAAATGCGGCGCTGATATCCCGATAGAGGAACGAGATC
CCGAGGAAGTCAGGCAGATTTCCGGAGTTCGGACCGCTCCATCCAATGTGCCTGTCTTTAATCCCGCTTTCGACATTACA
CCTCATGATCTCATTTCAGGAATCATCACAGAAAAAGGGATTATGACCGGAAACTATGAAGAAGAGATTGAACAGCTGTT
TAAAGGAGAAAAGGTGCATTAA

Upstream 100 bases:

>100_bases
CTCGAAACCGGCACAGCCTTTATCGAAAAACGTTCGGAATTCAAAACGATAACAGATGTTATTGAATTATTTAAGTTACT
TGTAAAGGAATGATCTATTC

Downstream 100 bases:

>100_bases
AAAACCGCTGGACTTTGCTCATTTGGAGCCGAGTCAGCGGTTTTTTTGTTTCTTTTTGTTAACATTGATGATTTCATCTT
TTTGAGCGCTTGATAAGATA

Product: methylthioribose-1-phosphate isomerase

Products: NA

Alternate protein names: M1Pi; MTR-1-P isomerase; S-methyl-5-thioribose-1-phosphate isomerase

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTHSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITAAFGLALAAKDIETDNVTEFR
RRLEDIKQYLNSSRPTAINLSWALERLSHSVENAISVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTIC
NAGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGAD
RIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQISGVRTAPSNVPVFNPAFDIT
PHDLISGIITEKGIMTGNYEEEIEQLFKGEKVH

Sequences:

>Translated_353_residues
MTHSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITAAFGLALAAKDIETDNVTEFR
RRLEDIKQYLNSSRPTAINLSWALERLSHSVENAISVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTIC
NAGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGAD
RIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQISGVRTAPSNVPVFNPAFDIT
PHDLISGIITEKGIMTGNYEEEIEQLFKGEKVH
>Mature_352_residues
THSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITAAFGLALAAKDIETDNVTEFRR
RLEDIKQYLNSSRPTAINLSWALERLSHSVENAISVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTICN
AGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGADR
IAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQISGVRTAPSNVPVFNPAFDITP
HDLISGIITEKGIMTGNYEEEIEQLFKGEKVH

Specific function: Catalyzes the interconversion of methylthioribose-1- phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1- P)

COG id: COG0182

COG function: function code J; Predicted translation initiation factor 2B subunit, eIF-2B alpha/beta/delta family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily

Homologues:

Organism=Homo sapiens, GI72534748, Length=345, Percent_Identity=39.7101449275362, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI23943880, Length=331, Percent_Identity=36.8580060422961, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI78000158, Length=304, Percent_Identity=25.3289473684211, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI78000156, Length=304, Percent_Identity=25.3289473684211, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI187607323, Length=304, Percent_Identity=25.3289473684211, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17557462, Length=338, Percent_Identity=37.2781065088757, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17557123, Length=157, Percent_Identity=29.9363057324841, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325375, Length=404, Percent_Identity=37.1287128712871, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322878, Length=171, Percent_Identity=29.2397660818713, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21357667, Length=362, Percent_Identity=37.8453038674033, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24651647, Length=362, Percent_Identity=37.8453038674033, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI281364091, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24659399, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24659407, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922816, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358605, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNA_BACSU (O31662)

Other databases:

- EMBL:   AL009126
- PIR:   C69863
- RefSeq:   NP_389238.1
- PDB:   2YRF
- PDB:   2YVK
- PDBsum:   2YRF
- PDBsum:   2YVK
- ProteinModelPortal:   O31662
- SMR:   O31662
- EnsemblBacteria:   EBBACT00000001624
- GeneID:   939341
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13550
- NMPDR:   fig|224308.1.peg.1357
- GenoList:   BSU13550
- GeneTree:   EBGT00050000000839
- HOGENOM:   HBG682649
- OMA:   LNQTKLP
- PhylomeDB:   O31662
- ProtClustDB:   PRK05720
- BioCyc:   BSUB:BSU13550-MONOMER
- BRENDA:   5.3.1.23
- HAMAP:   MF_01678
- InterPro:   IPR000649
- InterPro:   IPR005251
- InterPro:   IPR011559
- PANTHER:   PTHR10233
- TIGRFAMs:   TIGR00524
- TIGRFAMs:   TIGR00512

Pfam domain/function: PF01008 IF-2B

EC number: =5.3.1.23

Molecular weight: Translated: 38861; Mature: 38730

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 240-240 BINDING 94-94 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITA
CCCCCCCCCCCCHHHHHHEEEHHCCCCCCCCEEEEECHHHHHEEEEEEEECCCCCCHHHH
AFGLALAAKDIETDNVTEFRRRLEDIKQYLNSSRPTAINLSWALERLSHSVENAISVNEA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
KTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTICNAGSIATSRYGTALAPFYLA
HHHHHEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCCHHHHHHHC
KQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGAD
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCEEEEEEECHHHHHHHHHHCEEEEEEEHH
RIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQI
HHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCEECCCCCCCCCCCHHHHHHH
SGVRTAPSNVPVFNPAFDITPHDLISGIITEKGIMTGNYEEEIEQLFKGEKVH
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
THSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITA
CCCCCCCCCCCHHHHHHEEEHHCCCCCCCCEEEEECHHHHHEEEEEEEECCCCCCHHHH
AFGLALAAKDIETDNVTEFRRRLEDIKQYLNSSRPTAINLSWALERLSHSVENAISVNEA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
KTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTICNAGSIATSRYGTALAPFYLA
HHHHHEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCCHHHHHHHC
KQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGAD
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCEEEEEEECHHHHHHHHHHCEEEEEEEHH
RIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQI
HHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCEECCCCCCCCCCCHHHHHHH
SGVRTAPSNVPVFNPAFDITPHDLISGIITEKGIMTGNYEEEIEQLFKGEKVH
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921; 14551435