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The map label for this gene is hmp

Identifier: 16078369

GI number: 16078369

Start: 1372792

End: 1373991

Strand: Direct

Name: hmp

Synonym: BSU13040

Alternate gene names: 16078369

Gene position: 1372792-1373991 (Clockwise)

Preceding gene: 50812222

Following gene: 16078371

Centisome position: 32.56

GC content: 44.17

Gene sequence:

>1200_bases
ATGTTAGATAACAAAACAATCGAAATTATTAAAAGCACTGTACCCGTATTGCAACAGCATGGAGAAACAATCACTGGCCG
TTTTTACGATCGGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAAAAAACACAGC
GAACTGCACTGGCAAATGCCGTTATTGCAGCTGCGGCAAACATTGACCAATTAGGAAACATCATTCCGGTTGTCAAACAA
ATCGGCCACAAGCACCGCAGTATCGGAATTAAGCCTGAGCACTACCCAATCGTCGGTAAATATTTGCTGATTGCGATTAA
AGATGTATTGGGAGACGCTGCAACTCCTGACATTATGCAGGCATGGGAAAAAGCGTACGGAGTCATTGCTGACGCGTTCA
TCGGAATTGAAAAAGACATGTATGAACAGGCTGAAGAGCAAGCCGGCGGCTGGAAGGAGTATAAGCCATTCGTTATCGCA
AAAAAAGAGCGGGAAAGCAAAGAAATTACATCCTTCTATTTGAAACCGGAAGACAGCAAGCCGTTACCTGAATTCCAAGC
AGGGCAATATATCAGCATCAAAGTGCGGATTCCTGATTCTGAATATACGCATATCCGCCAGTACAGCCTGTCTGATATGC
CGGGAAAAGACTATTATCGAATTTCAGTGAAGAAAGATGGTGTCGTGTCTTCCTACTTACACGATGGGCTGCAGGAGGGA
GATTCAATAGAGATCAGCGCACCGGCAGGGGACTTTGTTTTAGATCATGCATCACAAAAAGATCTCGTTTTGATCAGTGC
CGGAGTTGGCATTACACCAATGATCAGCATGCTGAAAACATCTGTTTCCAAACAGCCTGAACGGCAAATTCTGTTCATTC
ATGCGGCGAAAAACAGTGAATATCATGCATTGCGCCATGAAGTAGAAGAAGCAGCAAAACATTCTGCTGTTAAAACAGCA
TTTGTGTATCGCGAGCCGACAGAGGAAGACCGTGCAGGTGATCTGCATTTTCATGAAGGACAGATTGATCAGCAATTCCT
CAAAGAACTGATTGCCAACACAGATGCGGATTACTATATTTGCGGTTCACCGTCGTTTATTACGGCAATGCATAAGCTTG
TATCAGAGCTCGGATCAGCGCCGGAGTCTATCCATTACGAGCTATTCGGCCCGCAATTAAGTTTGGCGCAGTCCGTTTGA

Upstream 100 bases:

>100_bases
ACTTTATTAAAGATTCATTTTAGATATATCTTTTATTCGTAAGATCATGTATTTTAAAGATATATTTTAAATACATCTTT
TCGAAAGGATTGTTTATAAA

Downstream 100 bases:

>100_bases
ACACATACATGAAACTCTTCTCCTTTCAGAGAAGAGTTTTTTATTGAGATATAAGAAGGCAGACAAGCGCCGGTTTCTAC
ATCCGGACAATCACAGACCC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLDNKTIEIIKSTVPVLQQHGETITGRFYDRMFQDHPELLNIFNQTNQKKKTQRTALANAVIAAAANIDQLGNIIPVVKQ
IGHKHRSIGIKPEHYPIVGKYLLIAIKDVLGDAATPDIMQAWEKAYGVIADAFIGIEKDMYEQAEEQAGGWKEYKPFVIA
KKERESKEITSFYLKPEDSKPLPEFQAGQYISIKVRIPDSEYTHIRQYSLSDMPGKDYYRISVKKDGVVSSYLHDGLQEG
DSIEISAPAGDFVLDHASQKDLVLISAGVGITPMISMLKTSVSKQPERQILFIHAAKNSEYHALRHEVEEAAKHSAVKTA
FVYREPTEEDRAGDLHFHEGQIDQQFLKELIANTDADYYICGSPSFITAMHKLVSELGSAPESIHYELFGPQLSLAQSV

Sequences:

>Translated_399_residues
MLDNKTIEIIKSTVPVLQQHGETITGRFYDRMFQDHPELLNIFNQTNQKKKTQRTALANAVIAAAANIDQLGNIIPVVKQ
IGHKHRSIGIKPEHYPIVGKYLLIAIKDVLGDAATPDIMQAWEKAYGVIADAFIGIEKDMYEQAEEQAGGWKEYKPFVIA
KKERESKEITSFYLKPEDSKPLPEFQAGQYISIKVRIPDSEYTHIRQYSLSDMPGKDYYRISVKKDGVVSSYLHDGLQEG
DSIEISAPAGDFVLDHASQKDLVLISAGVGITPMISMLKTSVSKQPERQILFIHAAKNSEYHALRHEVEEAAKHSAVKTA
FVYREPTEEDRAGDLHFHEGQIDQQFLKELIANTDADYYICGSPSFITAMHKLVSELGSAPESIHYELFGPQLSLAQSV
>Mature_399_residues
MLDNKTIEIIKSTVPVLQQHGETITGRFYDRMFQDHPELLNIFNQTNQKKKTQRTALANAVIAAAANIDQLGNIIPVVKQ
IGHKHRSIGIKPEHYPIVGKYLLIAIKDVLGDAATPDIMQAWEKAYGVIADAFIGIEKDMYEQAEEQAGGWKEYKPFVIA
KKERESKEITSFYLKPEDSKPLPEFQAGQYISIKVRIPDSEYTHIRQYSLSDMPGKDYYRISVKKDGVVSSYLHDGLQEG
DSIEISAPAGDFVLDHASQKDLVLISAGVGITPMISMLKTSVSKQPERQILFIHAAKNSEYHALRHEVEEAAKHSAVKTA
FVYREPTEEDRAGDLHFHEGQIDQQFLKELIANTDADYYICGSPSFITAMHKLVSELGSAPESIHYELFGPQLSLAQSV

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=394, Percent_Identity=40.6091370558376, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1788104, Length=241, Percent_Identity=25.3112033195021, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787658, Length=255, Percent_Identity=22.7450980392157, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787098, Length=140, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=416, Percent_Identity=43.75, Blast_Score=293, Evalue=4e-80,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): HMP_BACSU (P49852)

Other databases:

- EMBL:   D78189
- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   B69642
- RefSeq:   NP_389187.1
- ProteinModelPortal:   P49852
- SMR:   P49852
- EnsemblBacteria:   EBBACT00000003304
- GeneID:   939891
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13040
- NMPDR:   fig|224308.1.peg.1306
- GenoList:   BSU13040
- GeneTree:   EBGT00050000001043
- HOGENOM:   HBG623097
- OMA:   QIGHKHR
- PhylomeDB:   P49852
- ProtClustDB:   PRK13289
- BioCyc:   BSUB:BSU13040-MONOMER
- BRENDA:   1.14.12.17
- HAMAP:   MF_01252
- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.10.490.10
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1; SSF46458 Globin_like; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.14.12.17

Molecular weight: Translated: 44729; Mature: 44729

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS51384 FAD_FR; PS01033 GLOBIN

Important sites: ACT_SITE 95-95 ACT_SITE 137-137 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNKTIEIIKSTVPVLQQHGETITGRFYDRMFQDHPELLNIFNQTNQKKKTQRTALANA
CCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
VIAAAANIDQLGNIIPVVKQIGHKHRSIGIKPEHYPIVGKYLLIAIKDVLGDAATPDIMQ
HHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AWEKAYGVIADAFIGIEKDMYEQAEEQAGGWKEYKPFVIAKKERESKEITSFYLKPEDSK
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHEEEECCCCCC
PLPEFQAGQYISIKVRIPDSEYTHIRQYSLSDMPGKDYYRISVKKDGVVSSYLHDGLQEG
CCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
DSIEISAPAGDFVLDHASQKDLVLISAGVGITPMISMLKTSVSKQPERQILFIHAAKNSE
CCEEEECCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
YHALRHEVEEAAKHSAVKTAFVYREPTEEDRAGDLHFHEGQIDQQFLKELIANTDADYYI
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCEEE
CGSPSFITAMHKLVSELGSAPESIHYELFGPQLSLAQSV
ECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCC
>Mature Secondary Structure
MLDNKTIEIIKSTVPVLQQHGETITGRFYDRMFQDHPELLNIFNQTNQKKKTQRTALANA
CCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
VIAAAANIDQLGNIIPVVKQIGHKHRSIGIKPEHYPIVGKYLLIAIKDVLGDAATPDIMQ
HHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AWEKAYGVIADAFIGIEKDMYEQAEEQAGGWKEYKPFVIAKKERESKEITSFYLKPEDSK
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHEEEECCCCCC
PLPEFQAGQYISIKVRIPDSEYTHIRQYSLSDMPGKDYYRISVKKDGVVSSYLHDGLQEG
CCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
DSIEISAPAGDFVLDHASQKDLVLISAGVGITPMISMLKTSVSKQPERQILFIHAAKNSE
CCEEEECCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
YHALRHEVEEAAKHSAVKTAFVYREPTEEDRAGDLHFHEGQIDQQFLKELIANTDADYYI
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCEEE
CGSPSFITAMHKLVSELGSAPESIHYELFGPQLSLAQSV
ECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8682784; 9384377