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The map label for this gene is proG

Identifier: 16078356

GI number: 16078356

Start: 1359454

End: 1360272

Strand: Direct

Name: proG

Synonym: BSU12910

Alternate gene names: 16078356

Gene position: 1359454-1360272 (Clockwise)

Preceding gene: 255767299

Following gene: 255767301

Centisome position: 32.25

GC content: 46.64

Gene sequence:

>819_bases
ATGGAACAGATTGGATTGATTGGATATGGCAGCATGGCGGATATGATCGCTAGACAGCTGTTGAAACATGAACAAATAAA
AGAGAATGAGCTATTTATTGAGACGAGAACGAAGGGGGAGCGGCTGCGGGCACTCATGTCAGATTATCCAAACGTATCGG
CTGATCCGTTGGAGAATTGGGCAAATACGTGCCAATTGATTTTGATATGCGTCCCGCCACTCCATGTCATCGAAACAATG
CGCCGTCTGTACCCGTATGTGAACAGAAATACTCACATCGTATCCATAGCTGCCGGTGTGCCGCTGCGTCTTTTGGAGGC
AGAAACAGAAGCCGGTATCTCACGTGTTATTCCTGCCATTACGTCCGAAGCAGAAGCCGGCATCTCACTTGTCGTTCACA
GTGAAGCACTGGCTGCAGAGAAAAAAGAACGTCTGAATGAATTGTTATCGGTTTTCAGCCGTGTACGGGAAATCAAAGAA
TCGAATTTAGATGTGGCCAGCAATTTAACGAGCTCGGCACCGGGATTCATAGCTGCAATCTTTGAAGAACTGGCACTGTC
TGCCGTCAGAAACAGCAGCCTGTCCAAAGAAGAAGCCTTTGATTTTCTGATTCATTCGCTATACGGAACTGGCAAGATGC
TGATCGAAAAAAATATGTCTTTCGAGGAGACGCTGGAACGTGTTGCAACCAAAGGCGGAATAACGGGAGAGGGAGCTGAG
GTGATACACGCCTCTGTGCCGGACGTTTTTGATGAGGTATTTGAAAGAACCCTGAGAAAATATGAACTGCTTACAGAACA
GGTTGGTAAACAAACATGA

Upstream 100 bases:

>100_bases
ATAAACAAAAACTGTGGGATAAAAATGAAAAGATTATGGGAAATTGTGAAAAAGTGCGGAAAACGGTACACTTCATATTG
TATATGAAAGGACTGAGTCG

Downstream 100 bases:

>100_bases
ATCCTTGAAAGAGGATTCTTTTTTTATCACTGAATGATTGAGATTTTTCCCAGTTATATTGCATTTTTCCTCTTTTTTTA
ATATAATTTGTTAGAATATT

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 3; P5CR 3

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MEQIGLIGYGSMADMIARQLLKHEQIKENELFIETRTKGERLRALMSDYPNVSADPLENWANTCQLILICVPPLHVIETM
RRLYPYVNRNTHIVSIAAGVPLRLLEAETEAGISRVIPAITSEAEAGISLVVHSEALAAEKKERLNELLSVFSRVREIKE
SNLDVASNLTSSAPGFIAAIFEELALSAVRNSSLSKEEAFDFLIHSLYGTGKMLIEKNMSFEETLERVATKGGITGEGAE
VIHASVPDVFDEVFERTLRKYELLTEQVGKQT

Sequences:

>Translated_272_residues
MEQIGLIGYGSMADMIARQLLKHEQIKENELFIETRTKGERLRALMSDYPNVSADPLENWANTCQLILICVPPLHVIETM
RRLYPYVNRNTHIVSIAAGVPLRLLEAETEAGISRVIPAITSEAEAGISLVVHSEALAAEKKERLNELLSVFSRVREIKE
SNLDVASNLTSSAPGFIAAIFEELALSAVRNSSLSKEEAFDFLIHSLYGTGKMLIEKNMSFEETLERVATKGGITGEGAE
VIHASVPDVFDEVFERTLRKYELLTEQVGKQT
>Mature_272_residues
MEQIGLIGYGSMADMIARQLLKHEQIKENELFIETRTKGERLRALMSDYPNVSADPLENWANTCQLILICVPPLHVIETM
RRLYPYVNRNTHIVSIAAGVPLRLLEAETEAGISRVIPAITSEAEAGISLVVHSEALAAEKKERLNELLSVFSRVREIKE
SNLDVASNLTSSAPGFIAAIFEELALSAVRNSSLSKEEAFDFLIHSLYGTGKMLIEKNMSFEETLERVATKGGITGEGAE
VIHASVPDVFDEVFERTLRKYELLTEQVGKQT

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family

Homologues:

Organism=Homo sapiens, GI198041662, Length=237, Percent_Identity=24.4725738396624, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786585, Length=243, Percent_Identity=23.0452674897119, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17569021, Length=237, Percent_Identity=25.3164556962025, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): P5CR3_BACSU (Q00777)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- EMBL:   X56678
- PIR:   F69855
- RefSeq:   NP_389174.1
- ProteinModelPortal:   Q00777
- SMR:   Q00777
- EnsemblBacteria:   EBBACT00000002011
- GeneID:   936776
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12910
- NMPDR:   fig|224308.1.peg.1293
- GenoList:   BSU12910
- GeneTree:   EBGT00070000032098
- HOGENOM:   HBG344671
- OMA:   EIILYSS
- ProtClustDB:   PRK06928
- BioCyc:   BSUB:BSU12910-MONOMER
- BRENDA:   1.5.1.2
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11645
- PIRSF:   PIRSF000193

Pfam domain/function: PF03807 F420_oxidored; SSF48179 6DGDH_C_like

EC number: =1.5.1.2

Molecular weight: Translated: 30203; Mature: 30203

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQIGLIGYGSMADMIARQLLKHEQIKENELFIETRTKGERLRALMSDYPNVSADPLENW
CCCCCEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCHHHHH
ANTCQLILICVPPLHVIETMRRLYPYVNRNTHIVSIAAGVPLRLLEAETEAGISRVIPAI
HHHHHEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
TSEAEAGISLVVHSEALAAEKKERLNELLSVFSRVREIKESNLDVASNLTSSAPGFIAAI
HHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
FEELALSAVRNSSLSKEEAFDFLIHSLYGTGKMLIEKNMSFEETLERVATKGGITGEGAE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCHH
VIHASVPDVFDEVFERTLRKYELLTEQVGKQT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQIGLIGYGSMADMIARQLLKHEQIKENELFIETRTKGERLRALMSDYPNVSADPLENW
CCCCCEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCHHHHH
ANTCQLILICVPPLHVIETMRRLYPYVNRNTHIVSIAAGVPLRLLEAETEAGISRVIPAI
HHHHHEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
TSEAEAGISLVVHSEALAAEKKERLNELLSVFSRVREIKESNLDVASNLTSSAPGFIAAI
HHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
FEELALSAVRNSSLSKEEAFDFLIHSLYGTGKMLIEKNMSFEETLERVATKGGITGEGAE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCHH
VIHASVPDVFDEVFERTLRKYELLTEQVGKQT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 1766370; 11418582