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The map label for this gene is xpf

Identifier: 16078321

GI number: 16078321

Start: 1324471

End: 1324980

Strand: Direct

Name: xpf

Synonym: BSU12560

Alternate gene names: 16078321

Gene position: 1324471-1324980 (Clockwise)

Preceding gene: 16078320

Following gene: 16078322

Centisome position: 31.42

GC content: 42.94

Gene sequence:

>510_bases
ATGCAAGACTTACTATTTGAATATAAACGCACGCTCAAACAAACAAGAATACAATATAAACCGCTCGCTGAGGCAGATGA
ATCCGTGCTCTCAGCTGAAGAGCTGAAGGATAAAAAAATCATCAGAAATATGATTACTGATCTTGAATATGTAACAGAAT
GGCTTGAAAAAGGAAGGCAGCCCGGCATCAGACGGGCGATTGACCGGCGTGATGTTTACCAGCGGCTGATGATCAAGGAC
CCGAGAATCATCGAATCATTTTCCAGCGCTATGATGTTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGATAGAAT
TCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAAC
GGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAG
CAGGAAGAAATGAATCGATCACTTGCCTGA

Upstream 100 bases:

>100_bases
CGTCCAAGACGGAAAGCCTGCGGACACTGATCAACTGCACAGCATTTGTGCGTTGATTGGTGTCCGTTTTTTATTTGCCA
AAAATGAGGAGGATCATAGA

Downstream 100 bases:

>100_bases
AAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACACTAAGCGGCTGGCTGATCAGCCGCTTTTATGAATA
AACAACCATGCTGGAGGTGG

Product: positive control sigma-like factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MQDLLFEYKRTLKQTRIQYKPLAEADESVLSAEELKDKKIIRNMITDLEYVTEWLEKGRQPGIRRAIDRRDVYQRLMIKD
PRIIESFSSAMMFEPDGQVSEEDRDRIREALALLTDREKEMFLLHKVECFSYERIADLLGVKKSTVQTTIKRASLKMQRQ
QEEMNRSLA

Sequences:

>Translated_169_residues
MQDLLFEYKRTLKQTRIQYKPLAEADESVLSAEELKDKKIIRNMITDLEYVTEWLEKGRQPGIRRAIDRRDVYQRLMIKD
PRIIESFSSAMMFEPDGQVSEEDRDRIREALALLTDREKEMFLLHKVECFSYERIADLLGVKKSTVQTTIKRASLKMQRQ
QEEMNRSLA
>Mature_169_residues
MQDLLFEYKRTLKQTRIQYKPLAEADESVLSAEELKDKKIIRNMITDLEYVTEWLEKGRQPGIRRAIDRRDVYQRLMIKD
PRIIESFSSAMMFEPDGQVSEEDRDRIREALALLTDREKEMFLLHKVECFSYERIADLLGVKKSTVQTTIKRASLKMQRQ
QEEMNRSLA

Specific function: Positive regulatory protein that acts at the late promoter PL

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCF_BACSU (P39784)

Other databases:

- EMBL:   Z34287
- EMBL:   Z70177
- EMBL:   AL009126
- PIR:   I40413
- RefSeq:   NP_389138.1
- ProteinModelPortal:   P39784
- SMR:   P39784
- EnsemblBacteria:   EBBACT00000000059
- GeneID:   936474
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12560
- NMPDR:   fig|224308.1.peg.1257
- GenoList:   BSU12560
- GeneTree:   EBGT00050000001233
- HOGENOM:   HBG314082
- OMA:   EREKEVY
- ProtClustDB:   PRK06930
- BioCyc:   BSUB:BSU12560-MONOMER
- InterPro:   IPR013249
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF08281 Sigma70_r4_2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 20092; Mature: 20092

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00622 HTH_LUXR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLLFEYKRTLKQTRIQYKPLAEADESVLSAEELKDKKIIRNMITDLEYVTEWLEKGRQ
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGIRRAIDRRDVYQRLMIKDPRIIESFSSAMMFEPDGQVSEEDRDRIREALALLTDREKE
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
MFLLHKVECFSYERIADLLGVKKSTVQTTIKRASLKMQRQQEEMNRSLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQDLLFEYKRTLKQTRIQYKPLAEADESVLSAEELKDKKIIRNMITDLEYVTEWLEKGRQ
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGIRRAIDRRDVYQRLMIKDPRIIESFSSAMMFEPDGQVSEEDRDRIREALALLTDREKE
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
MFLLHKVECFSYERIADLLGVKKSTVQTTIKRASLKMQRQQEEMNRSLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083174; 9384377