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The map label for this gene is thiF

Identifier: 16078235

GI number: 16078235

Start: 1245808

End: 1246818

Strand: Direct

Name: thiF

Synonym: BSU11700

Alternate gene names: 16078235

Gene position: 1245808-1246818 (Clockwise)

Preceding gene: 16078234

Following gene: 16078236

Centisome position: 29.55

GC content: 51.04

Gene sequence:

>1011_bases
ATGACAGGCAGGTATTCAAGGCAGGAGCTGTTTGCTCCGATCGGCCCATCCGGCCAGAAAAAATTGAAAGAAGCGCGCGC
CGTGATTATCGGCGCCGGCGCGCTCGGAACAGCCAGTGCAGAAATGCTGGTGAGAGCGGGAGTCGGCTCTGTGAAAATTG
CCGACAGAGATTATGTTGAATGGAGCAACCTCCAGCGCCAGCAGCTTTATACAGAAGATGATGTCAAAAAAGAAATGCCG
AAAGCAGCCGCTGCGGAGCGCCGTCTCCGTTCGATTAACAGCGATGTTGATGTGACCGGCCTTGTCATGGATGTGACAGC
AGAAAATATCTTCGAGCTGATTAGGGATGCATCGATTATTGTGGATGCGGCTGATAATTTTGAAACCCGCCTGATCGTAA
ATGATGCTGCGGTAAAGGAAGGGATTCCTTTTCTTTACGGTGCCTGTGTAGGGAGCTACGGTCTTACCTTTACCGTGGTG
CCGGGTTCTACTCCTTGCCTGCATTGCTTGCTCGATGCACTTCCGATTGGCGGAGCAACATGTGACACAGCTGGCATCAT
CAGTCCGGCTGTTTTGCAGGTAGCAGTCTTTCAGGTAACAGATGCACTGAAACTGCTGACGGGAGAGGAGTGCGAGCCGG
TGCTGCGCTCCTTTGATTTGTGGAAAAACGAGCGGTCAGAAGTGAGAGCGGCCAGTCTCAAACATGACGCATGCCCAAGC
TGCGGCACAAAGGATTTTCCGTTTTTGTCTTATGAAAATCAAACAAAAGCGGCTGTATTGTGCGGCAGGAACACGGTGCA
GATCAGATCATCTATTACGAAAGAAGCGGATCTTGAAGCGCTTGCCGGTCAGCTGAGGCAGGCCGGGCTTGAAGTAGCTG
CGAACCCGTATTTAATTTCCTGCCGCTCGGATGATATGAAAATGGTGCTGTTCCGGGACGGCAGAGCTTTGATCCATGGA
ACAAATGATATTGCCCGGGCAAAATCCATTTATCATAAATGGATTGGGTAA

Upstream 100 bases:

>100_bases
TGAAGCTCGCAGTGGAAGCAGGGCGCCTTTCTTATGAGGCGGGCCGAATCCCGCTTAAGCAATACGGAACGGCAAGCAGT
CCGGGAGAAGGTCTTCCTGT

Downstream 100 bases:

>100_bases
TAAAAGGAGTGTAGTGTAATGAGTATATATAAAGCATTGACAATCGCCGGATCAGATTCAGGCGGCGGCGCAGGGATACA
GGCTGATATTAAAACCTTTC

Product: thiamine/molybdopterin biosynthesis ThiF/MoeB-like protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTGRYSRQELFAPIGPSGQKKLKEARAVIIGAGALGTASAEMLVRAGVGSVKIADRDYVEWSNLQRQQLYTEDDVKKEMP
KAAAAERRLRSINSDVDVTGLVMDVTAENIFELIRDASIIVDAADNFETRLIVNDAAVKEGIPFLYGACVGSYGLTFTVV
PGSTPCLHCLLDALPIGGATCDTAGIISPAVLQVAVFQVTDALKLLTGEECEPVLRSFDLWKNERSEVRAASLKHDACPS
CGTKDFPFLSYENQTKAAVLCGRNTVQIRSSITKEADLEALAGQLRQAGLEVAANPYLISCRSDDMKMVLFRDGRALIHG
TNDIARAKSIYHKWIG

Sequences:

>Translated_336_residues
MTGRYSRQELFAPIGPSGQKKLKEARAVIIGAGALGTASAEMLVRAGVGSVKIADRDYVEWSNLQRQQLYTEDDVKKEMP
KAAAAERRLRSINSDVDVTGLVMDVTAENIFELIRDASIIVDAADNFETRLIVNDAAVKEGIPFLYGACVGSYGLTFTVV
PGSTPCLHCLLDALPIGGATCDTAGIISPAVLQVAVFQVTDALKLLTGEECEPVLRSFDLWKNERSEVRAASLKHDACPS
CGTKDFPFLSYENQTKAAVLCGRNTVQIRSSITKEADLEALAGQLRQAGLEVAANPYLISCRSDDMKMVLFRDGRALIHG
TNDIARAKSIYHKWIG
>Mature_335_residues
TGRYSRQELFAPIGPSGQKKLKEARAVIIGAGALGTASAEMLVRAGVGSVKIADRDYVEWSNLQRQQLYTEDDVKKEMPK
AAAAERRLRSINSDVDVTGLVMDVTAENIFELIRDASIIVDAADNFETRLIVNDAAVKEGIPFLYGACVGSYGLTFTVVP
GSTPCLHCLLDALPIGGATCDTAGIISPAVLQVAVFQVTDALKLLTGEECEPVLRSFDLWKNERSEVRAASLKHDACPSC
GTKDFPFLSYENQTKAAVLCGRNTVQIRSSITKEADLEALAGQLRQAGLEVAANPYLISCRSDDMKMVLFRDGRALIHGT
NDIARAKSIYHKWIG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family

Homologues:

Organism=Homo sapiens, GI7657339, Length=172, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI38045948, Length=170, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082356, Length=242, Percent_Identity=36.7768595041322, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787048, Length=241, Percent_Identity=29.045643153527, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17540406, Length=206, Percent_Identity=34.9514563106796, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71984476, Length=178, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=247, Percent_Identity=29.9595141700405, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320598, Length=172, Percent_Identity=29.6511627906977, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325323, Length=161, Percent_Identity=31.055900621118, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24582879, Length=207, Percent_Identity=30.4347826086957, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28573937, Length=172, Percent_Identity=30.2325581395349, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24660640, Length=155, Percent_Identity=27.741935483871, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIF_BACSU (O31619)

Other databases:

- EMBL:   AL009126
- PIR:   E69845
- RefSeq:   NP_389052.1
- HSSP:   P12282
- ProteinModelPortal:   O31619
- SMR:   O31619
- EnsemblBacteria:   EBBACT00000001460
- GeneID:   939816
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11700
- NMPDR:   fig|224308.1.peg.1171
- GenoList:   BSU11700
- GeneTree:   EBGT00070000032023
- HOGENOM:   HBG535305
- OMA:   IRPPHKE
- PhylomeDB:   O31619
- ProtClustDB:   PRK07688
- BioCyc:   BSUB:BSU11700-MONOMER
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF; SSF69572 MoeB

EC number: 2.7.7.- [C]

Molecular weight: Translated: 36400; Mature: 36269

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 7-7 BINDING 34-34 BINDING 55-55 BINDING 66-66 BINDING 81-81 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRYSRQELFAPIGPSGQKKLKEARAVIIGAGALGTASAEMLVRAGVGSVKIADRDYVE
CCCCCCCHHHCCCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCHH
WSNLQRQQLYTEDDVKKEMPKAAAAERRLRSINSDVDVTGLVMDVTAENIFELIRDASII
HHCCHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHCCEEE
VDAADNFETRLIVNDAAVKEGIPFLYGACVGSYGLTFTVVPGSTPCLHCLLDALPIGGAT
EECCCCCCEEEEEEHHHHHHCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVLQVAVFQVTDALKLLTGEECEPVLRSFDLWKNERSEVRAASLKHDACPS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
CGTKDFPFLSYENQTKAAVLCGRNTVQIRSSITKEADLEALAGQLRQAGLEVAANPYLIS
CCCCCCCEEEECCCCCEEEEECCCEEEHHHHHCHHHHHHHHHHHHHHCCHHCCCCCEEEE
CRSDDMKMVLFRDGRALIHGTNDIARAKSIYHKWIG
ECCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGRYSRQELFAPIGPSGQKKLKEARAVIIGAGALGTASAEMLVRAGVGSVKIADRDYVE
CCCCCCHHHCCCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCHH
WSNLQRQQLYTEDDVKKEMPKAAAAERRLRSINSDVDVTGLVMDVTAENIFELIRDASII
HHCCHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHCCEEE
VDAADNFETRLIVNDAAVKEGIPFLYGACVGSYGLTFTVVPGSTPCLHCLLDALPIGGAT
EECCCCCCEEEEEEHHHHHHCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVLQVAVFQVTDALKLLTGEECEPVLRSFDLWKNERSEVRAASLKHDACPS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
CGTKDFPFLSYENQTKAAVLCGRNTVQIRSSITKEADLEALAGQLRQAGLEVAANPYLIS
CCCCCCCEEEECCCCCEEEEECCCEEEHHHHHCHHHHHHHHHHHHHHCCHHCCCCCEEEE
CRSDDMKMVLFRDGRALIHGTNDIARAKSIYHKWIG
ECCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377