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The map label for this gene is yisY

Identifier: 16078154

GI number: 16078154

Start: 1169043

End: 1169849

Strand: Direct

Name: yisY

Synonym: BSU10900

Alternate gene names: 16078154

Gene position: 1169043-1169849 (Clockwise)

Preceding gene: 50812213

Following gene: 16078158

Centisome position: 27.73

GC content: 47.46

Gene sequence:

>807_bases
ATGGGGCATTACATCAAAACCGAGGAGCATGTGACACTGTTTGTAGAGGATATCGGACATGGAAGGCCGATCATCTTTTT
GCACGGGTGGCCGTTGAATCATAAGATGTTTGAATATCAAATGAATGAGCTTCCGAAAAGGGGATTTCGTTTTATCGGCG
TTGATTTGCGGGGATATGGGCAATCTGACCGCCCTTGGGAAGGCTACGATTATGACACGATGGCCGATGATGTGAAAGCA
GTCATTTATACGCTGCAGCTTGAGAATGCGATTCTTGCCGGTTTTTCAATGGGCGGCGCAATTGCAATCCGTTATATGGC
AAGGCATGAAGGAGCCGATGTTGATAAGCTGATTTTACTGTCTGCGGCGGCCCCCGCGTTTACAAAACGCCCGGGTTATC
CGTACGGGATGAGGAAGCAGGATATTGACGATATGATTGAATTGTTCAAAGCTGATCGGCCCAAAACACTGGCTGATTTA
GGGAAACAGTTTTTTGAGAAAAAAGTGTCTCCAGAGCTTAGGCAGTGGTTTCTCAATCTGATGCTGGAGGCTTCCTCCTA
CGGGACGATCCACTCGGGCATCGCATTAAGAGACGAAGATCTCAGAAAGGAACTTGCTGCAATCAAGGTGCCGACGCTGA
TCCTGCACGGGAGAAAGGATAGAATTGCGCCGTTTGATTTTGCGAAAGAATTGAAGCGCGGCATCAAACAGTCGGAATTG
GTTCCGTTTGCAAACAGCGGGCACGGAGCATTTTATGAGGAAAAAGAGAAGATCAACAGTTTGATTGCGCAGTTCTCCAA
CTCATAA

Upstream 100 bases:

>100_bases
GTACAGCAGCCCGCTTGTTTTGTAAGCATAAATGGAAAGCTTCCTATACGTCAAGGCGTTTACAGGCCGAAAAAGCCAAA
GGGAACAGGAGGAATGCGGA

Downstream 100 bases:

>100_bases
GAAAAAACCCGACATTGATCGGGTTTTTTATATAATTCCTCTGTTTTGCAGTGCGTTAATGATGAGATCAGCTCCATCGG
AAATAGAGGTCTGGTCAGAC

Product: hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MGHYIKTEEHVTLFVEDIGHGRPIIFLHGWPLNHKMFEYQMNELPKRGFRFIGVDLRGYGQSDRPWEGYDYDTMADDVKA
VIYTLQLENAILAGFSMGGAIAIRYMARHEGADVDKLILLSAAAPAFTKRPGYPYGMRKQDIDDMIELFKADRPKTLADL
GKQFFEKKVSPELRQWFLNLMLEASSYGTIHSGIALRDEDLRKELAAIKVPTLILHGRKDRIAPFDFAKELKRGIKQSEL
VPFANSGHGAFYEEKEKINSLIAQFSNS

Sequences:

>Translated_268_residues
MGHYIKTEEHVTLFVEDIGHGRPIIFLHGWPLNHKMFEYQMNELPKRGFRFIGVDLRGYGQSDRPWEGYDYDTMADDVKA
VIYTLQLENAILAGFSMGGAIAIRYMARHEGADVDKLILLSAAAPAFTKRPGYPYGMRKQDIDDMIELFKADRPKTLADL
GKQFFEKKVSPELRQWFLNLMLEASSYGTIHSGIALRDEDLRKELAAIKVPTLILHGRKDRIAPFDFAKELKRGIKQSEL
VPFANSGHGAFYEEKEKINSLIAQFSNS
>Mature_267_residues
GHYIKTEEHVTLFVEDIGHGRPIIFLHGWPLNHKMFEYQMNELPKRGFRFIGVDLRGYGQSDRPWEGYDYDTMADDVKAV
IYTLQLENAILAGFSMGGAIAIRYMARHEGADVDKLILLSAAAPAFTKRPGYPYGMRKQDIDDMIELFKADRPKTLADLG
KQFFEKKVSPELRQWFLNLMLEASSYGTIHSGIALRDEDLRKELAAIKVPTLILHGRKDRIAPFDFAKELKRGIKQSELV
PFANSGHGAFYEEKEKINSLIAQFSNS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily

Homologues:

Organism=Escherichia coli, GI87081721, Length=272, Percent_Identity=28.3088235294118, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554012, Length=290, Percent_Identity=25.1724137931034, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YISY_BACSU (O06734)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   H69838
- RefSeq:   NP_388971.1
- HSSP:   P22862
- ProteinModelPortal:   O06734
- SMR:   O06734
- EnsemblBacteria:   EBBACT00000003579
- GeneID:   939790
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10900
- NMPDR:   fig|224308.1.peg.1090
- GenoList:   BSU10900
- GeneTree:   EBGT00070000032009
- HOGENOM:   HBG748003
- OMA:   PQHGFRC
- PhylomeDB:   O06734
- ProtClustDB:   CLSK2752365
- BioCyc:   BSUB:BSU10900-MONOMER
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30560; Mature: 30428

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: ACT_SITE 96-96 ACT_SITE 220-220 ACT_SITE 248-248

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHYIKTEEHVTLFVEDIGHGRPIIFLHGWPLNHKMFEYQMNELPKRGFRFIGVDLRGYG
CCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCC
QSDRPWEGYDYDTMADDVKAVIYTLQLENAILAGFSMGGAIAIRYMARHEGADVDKLILL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHEE
SAAAPAFTKRPGYPYGMRKQDIDDMIELFKADRPKTLADLGKQFFEKKVSPELRQWFLNL
CCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
MLEASSYGTIHSGIALRDEDLRKELAAIKVPTLILHGRKDRIAPFDFAKELKRGIKQSEL
HHHCCCCCCHHCCCEECHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHCCCHHHC
VPFANSGHGAFYEEKEKINSLIAQFSNS
CCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GHYIKTEEHVTLFVEDIGHGRPIIFLHGWPLNHKMFEYQMNELPKRGFRFIGVDLRGYG
CCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCC
QSDRPWEGYDYDTMADDVKAVIYTLQLENAILAGFSMGGAIAIRYMARHEGADVDKLILL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHEE
SAAAPAFTKRPGYPYGMRKQDIDDMIELFKADRPKTLADLGKQFFEKKVSPELRQWFLNL
CCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
MLEASSYGTIHSGIALRDEDLRKELAAIKVPTLILHGRKDRIAPFDFAKELKRGIKQSEL
HHHCCCCCCHHCCCEECHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHCCCHHHC
VPFANSGHGAFYEEKEKINSLIAQFSNS
CCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377