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The map label for this gene is yisS

Identifier: 16078148

GI number: 16078148

Start: 1164370

End: 1165398

Strand: Direct

Name: yisS

Synonym: BSU10850

Alternate gene names: 16078148

Gene position: 1164370-1165398 (Clockwise)

Preceding gene: 16078147

Following gene: 50812213

Centisome position: 27.62

GC content: 48.4

Gene sequence:

>1029_bases
GTGGAACATCAAGTAAGATGTGCAGTATTGGGATTAGGAAGGCTCGGTTATTATCATGCGAAAAATCTCGTCACCAGTGT
GCCGGGGGCAAAGCTGGTTTGTGTCGGTGATCCGTTAAAAGGGAGAGCGGAGCAGGTTGCCAGAGAACTCGGTATCGAAA
AATGGTCAGAGGACCCGTATGAAGTGTTAGAAGATCCCGGCATTGATGCTGTCATTATCGTAACGCCGACAAGCACACAT
GGTGATATGATCATCAAAGCAGCCGAGAACGGCAAACAGATCTTTGTTGAAAAACCGCTGACATTAAGCCTTGAGGAATC
AAAAGCAGCTTCTGAAAAAGTTAAGGAGACAGGTGTCATCTGCCAAGTCGGCTTTATGAGACGGTTCGATCCCGCATACG
CAGATGCCAAACGGCGGATCGACGCTGGAGAAATCGGCAAACCTATCTATTATAAAGGCTTTACGCGCGACCAAGGCGCG
CCTCCCGCAGAATTTATCAAACACAGCGGTGGAATTTTTATCGACTGTTCCATCCATGACTATGATATTGCCCGTTATTT
GCTAGGGGCGGAAATCACTTCTGTTTCAGGACACGGCAGGATTCTGAACAATCCGTTTATGGAGCAGTATGGCGATGTGG
ATCAGGCGCTGACGTATATTGAATTTGACTCGGGCGCAGCGGGGGACGTCGAGGCAAGCAGAACCTCTCCATACGGACAT
GACATCCGGGCGGAGGTGATCGGGACAGAGGGCAGTATTTTCATAGGGACATTGAGACATCAACATGTGACCATCCTATC
GGCTAAAGGGAGCAGTTTTGATATCATTCCAGACTTTCAAACTCGTTTTCATGAAGCCTACTGCTTGGAGCTTCAGCATT
TCGCCGAGTGTGTCCGGAATGGAAAAACACCGATTGTGACTGATATTGATGCGACGATCAATTTAGAAGTGGGTATCGCC
GCAACCAATTCCTTTCGAAACGGCATGCCGGTACAGCTAGATGTGAAGCGCGCTTATACAGGTATGTAA

Upstream 100 bases:

>100_bases
CGCAAAATGGCAGAGCGGATAGAACCGCTGTTTTTTTAGCTGTTAAGAGAAAACGCTTTCTCAAAGCGAGCCCGAAATGA
GATAAAGGAGAGGATGGGAC

Downstream 100 bases:

>100_bases
AAGCTAAGTATGTTGCATTTGAGGCTTTCCGCAAAAAAAGCAAGGGCATTTTAGCCCTTGCTTTCTCTTTTCTCATATAA
ATAGTACATATAATCAGTAG

Product: dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPYEVLEDPGIDAVIIVTPTSTH
GDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGA
PPAEFIKHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGH
DIRAEVIGTEGSIFIGTLRHQHVTILSAKGSSFDIIPDFQTRFHEAYCLELQHFAECVRNGKTPIVTDIDATINLEVGIA
ATNSFRNGMPVQLDVKRAYTGM

Sequences:

>Translated_342_residues
MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPYEVLEDPGIDAVIIVTPTSTH
GDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGA
PPAEFIKHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGH
DIRAEVIGTEGSIFIGTLRHQHVTILSAKGSSFDIIPDFQTRFHEAYCLELQHFAECVRNGKTPIVTDIDATINLEVGIA
ATNSFRNGMPVQLDVKRAYTGM
>Mature_342_residues
MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPYEVLEDPGIDAVIIVTPTSTH
GDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGA
PPAEFIKHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGH
DIRAEVIGTEGSIFIGTLRHQHVTILSAKGSSFDIIPDFQTRFHEAYCLELQHFAECVRNGKTPIVTDIDATINLEVGIA
ATNSFRNGMPVQLDVKRAYTGM

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family

Homologues:

Organism=Escherichia coli, GI87082405, Length=271, Percent_Identity=30.2583025830258, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081947, Length=206, Percent_Identity=25.7281553398058, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789848, Length=193, Percent_Identity=24.8704663212435, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YISS_BACSU (P40332)

Other databases:

- EMBL:   Y09476
- EMBL:   Z93940
- EMBL:   AL009126
- EMBL:   L08822
- PIR:   B69838
- RefSeq:   NP_388965.1
- ProteinModelPortal:   P40332
- SMR:   P40332
- EnsemblBacteria:   EBBACT00000002435
- GeneID:   939785
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10850
- NMPDR:   fig|224308.1.peg.1084
- GenoList:   BSU10850
- GeneTree:   EBGT00050000000829
- HOGENOM:   HBG612336
- OMA:   DYDIARY
- PhylomeDB:   P40332
- ProtClustDB:   CLSK248842
- BioCyc:   BSUB:BSU10850-MONOMER
- GO:   GO:0005488
- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37484; Mature: 37484

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPY
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCHHCCCCHH
EVLEDPGIDAVIIVTPTSTHGDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVI
HHHHCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCEEEEEHHHHHHHHHHHHCCCE
CQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGAPPAEFIKHSGGIFIDCSIHD
EEECHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCH
YDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGH
HHHHHHHHCCEEEEECCCCEECCCCHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCCC
DIRAEVIGTEGSIFIGTLRHQHVTILSAKGSSFDIIPDFQTRFHEAYCLELQHFAECVRN
CEEEEEECCCCCEEEEEECCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHC
GKTPIVTDIDATINLEVGIAATNSFRNGMPVQLDVKRAYTGM
CCCCEEEECCEEEEEEEEEEECCCCCCCCCEEEEHHHHCCCC
>Mature Secondary Structure
MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPY
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCHHCCCCHH
EVLEDPGIDAVIIVTPTSTHGDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVI
HHHHCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCEEEEEHHHHHHHHHHHHCCCE
CQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGAPPAEFIKHSGGIFIDCSIHD
EEECHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCH
YDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGH
HHHHHHHHCCEEEEECCCCEECCCCHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCCC
DIRAEVIGTEGSIFIGTLRHQHVTILSAKGSSFDIIPDFQTRFHEAYCLELQHFAECVRN
CEEEEEECCCCCEEEEEECCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHC
GKTPIVTDIDATINLEVGIAATNSFRNGMPVQLDVKRAYTGM
CCCCEEEECCEEEEEEEEEEECCCCCCCCCEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377; 8407808