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The map label for this gene is degA

Identifier: 16078147

GI number: 16078147

Start: 1163148

End: 1164161

Strand: Direct

Name: degA

Synonym: BSU10840

Alternate gene names: 16078147

Gene position: 1163148-1164161 (Clockwise)

Preceding gene: 16078146

Following gene: 16078148

Centisome position: 27.59

GC content: 47.63

Gene sequence:

>1014_bases
ATGAAAACAACAATTTACGATGTGGCAAAGGCGGCGGGTGTTTCGATCACAACTGTGTCCCGTGTGATAAACAATACGGG
GAGAATCAGCGATAAAACACGGCAGAAGGTCATGAATGTCATGAATGAAATGGCATACACGCCCAATGTCCACGCCGCGG
CGTTAACGGGAAAACGAACGAATATGATCGCTCTCGTGGCGCCTGATATTTCAAATCCGTTTTACGGCGAACTGGCCAAA
AGCATTGAGGAAAGAGCGGATGAGCTCGGTTTTCAAATGTTGATTTGCAGCACCGATTACGATCCGAAAAAAGAAACAAA
GTATTTTTCTGTGCTGAAACAGAAAAAGGTGGATGGCATTATTTTTGCAACAGGGATTGAAAGCCATGACAGTATGTCTG
CTTTGGAGGAAATCGCCAGTGAACAAATTCCGATTGCCATGATTTCTCAGGATAAGCCGCTTCTTCCGATGGATATTGTT
GTGATAGACGACGTTCGTGGCGGATATGAGGCTGCCAAGCATCTTCTGTCACTCGGCCACACGAACATCGCCTGCATTAT
CGGAGACGGGTCAACGACAGGCGAAAAAAATAGAATCAAAGGCTTCCGTCAGGCGATGGAGGAAGCGGGCGTGCCCATTG
ATGAATCACTTATCATCCAGACGCGGTTTTCCTTGGAAAGCGGCAAGGAAGAAGCGGGAAAATTGCTCGACAGAAATGCT
CCGACTGCTATTTTTGCCTTCAACGACGTATTGGCTTGTGCCGCCATACAAGCTGCGAGAATAAGAGGCATAAAAGTGCC
GGATGATCTTTCTATCATTGGTTTTGATAATACGATACTCGCAGAAATGGCGGCTCCGCCCCTGACAACCGTAGCCCAGC
CGATAAAAGAGATGGGGCGGAGCGTCATCGAACTGCTGGCAGAAGCAATAGAGGGAAAACGAAAGGCGAAACAAAAAATA
GTGCTCCCGCCTGAGCTTGTAGTCAGACATTCTACATCACCGCTCAACACATGA

Upstream 100 bases:

>100_bases
CATACTTTTCAAAACTCTTTAAGCAGATAGAAGGCATTTCACCAATTGAATACCGGAAAATTGTCAGCCGGAAAGTTCAA
TAACCAGACGAGGGATGAAC

Downstream 100 bases:

>100_bases
GCCCGCTAATGAGCGGGTTTTTTCATTATGACAGTTGAAGCCAAAAAAACATATTGCGTGTAAACCAAAGGCGGTGTAAA
ATATAACCATCTTTTGAGAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKTTIYDVAKAAGVSITTVSRVINNTGRISDKTRQKVMNVMNEMAYTPNVHAAALTGKRTNMIALVAPDISNPFYGELAK
SIEERADELGFQMLICSTDYDPKKETKYFSVLKQKKVDGIIFATGIESHDSMSALEEIASEQIPIAMISQDKPLLPMDIV
VIDDVRGGYEAAKHLLSLGHTNIACIIGDGSTTGEKNRIKGFRQAMEEAGVPIDESLIIQTRFSLESGKEEAGKLLDRNA
PTAIFAFNDVLACAAIQAARIRGIKVPDDLSIIGFDNTILAEMAAPPLTTVAQPIKEMGRSVIELLAEAIEGKRKAKQKI
VLPPELVVRHSTSPLNT

Sequences:

>Translated_337_residues
MKTTIYDVAKAAGVSITTVSRVINNTGRISDKTRQKVMNVMNEMAYTPNVHAAALTGKRTNMIALVAPDISNPFYGELAK
SIEERADELGFQMLICSTDYDPKKETKYFSVLKQKKVDGIIFATGIESHDSMSALEEIASEQIPIAMISQDKPLLPMDIV
VIDDVRGGYEAAKHLLSLGHTNIACIIGDGSTTGEKNRIKGFRQAMEEAGVPIDESLIIQTRFSLESGKEEAGKLLDRNA
PTAIFAFNDVLACAAIQAARIRGIKVPDDLSIIGFDNTILAEMAAPPLTTVAQPIKEMGRSVIELLAEAIEGKRKAKQKI
VLPPELVVRHSTSPLNT
>Mature_337_residues
MKTTIYDVAKAAGVSITTVSRVINNTGRISDKTRQKVMNVMNEMAYTPNVHAAALTGKRTNMIALVAPDISNPFYGELAK
SIEERADELGFQMLICSTDYDPKKETKYFSVLKQKKVDGIIFATGIESHDSMSALEEIASEQIPIAMISQDKPLLPMDIV
VIDDVRGGYEAAKHLLSLGHTNIACIIGDGSTTGEKNRIKGFRQAMEEAGVPIDESLIIQTRFSLESGKEEAGKLLDRNA
PTAIFAFNDVLACAAIQAARIRGIKVPDDLSIIGFDNTILAEMAAPPLTTVAQPIKEMGRSVIELLAEAIEGKRKAKQKI
VLPPELVVRHSTSPLNT

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=33.033033033033, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=33.433734939759, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=31.9148936170213, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789202, Length=334, Percent_Identity=31.7365269461078, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=32.5301204819277, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1786540, Length=338, Percent_Identity=29.8816568047337, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=29.8412698412698, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787906, Length=337, Percent_Identity=27.5964391691395, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=29.2834890965732, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=29.2834890965732, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=23.7341772151899, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=25.9587020648968, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=24.773413897281, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36596; Mature: 36596

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTIYDVAKAAGVSITTVSRVINNTGRISDKTRQKVMNVMNEMAYTPNVHAAALTGKRT
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
NMIALVAPDISNPFYGELAKSIEERADELGFQMLICSTDYDPKKETKYFSVLKQKKVDGI
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCE
IFATGIESHDSMSALEEIASEQIPIAMISQDKPLLPMDIVVIDDVRGGYEAAKHLLSLGH
EEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCC
TNIACIIGDGSTTGEKNRIKGFRQAMEEAGVPIDESLIIQTRFSLESGKEEAGKLLDRNA
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHCCHHHHHHHHCCCC
PTAIFAFNDVLACAAIQAARIRGIKVPDDLSIIGFDNTILAEMAAPPLTTVAQPIKEMGR
CEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHH
SVIELLAEAIEGKRKAKQKIVLPPELVVRHSTSPLNT
HHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCC
>Mature Secondary Structure
MKTTIYDVAKAAGVSITTVSRVINNTGRISDKTRQKVMNVMNEMAYTPNVHAAALTGKRT
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
NMIALVAPDISNPFYGELAKSIEERADELGFQMLICSTDYDPKKETKYFSVLKQKKVDGI
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCE
IFATGIESHDSMSALEEIASEQIPIAMISQDKPLLPMDIVVIDDVRGGYEAAKHLLSLGH
EEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCC
TNIACIIGDGSTTGEKNRIKGFRQAMEEAGVPIDESLIIQTRFSLESGKEEAGKLLDRNA
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHCCHHHHHHHHCCCC
PTAIFAFNDVLACAAIQAARIRGIKVPDDLSIIGFDNTILAEMAAPPLTTVAQPIKEMGR
CEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHH
SVIELLAEAIEGKRKAKQKIVLPPELVVRHSTSPLNT
HHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]