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The map label for this gene is yisQ

Identifier: 16078145

GI number: 16078145

Start: 1160776

End: 1162143

Strand: Reverse

Name: yisQ

Synonym: BSU10820

Alternate gene names: 16078145

Gene position: 1162143-1160776 (Counterclockwise)

Preceding gene: 50812212

Following gene: 50812211

Centisome position: 27.57

GC content: 45.39

Gene sequence:

>1368_bases
ATGAAACAAGCTGTCTTCAAATCAACTGCTTTGAAAAAACCCGCTGACAAAACGTTCTCATTATTTTCGCTTACTTGGCC
GATCTTTATTGAGGTCTCATTATATATGTTTATGGGTAATGCCGATACACTGATGCTCAGCCAATACTCGGATAACAGCG
TGGCAGCAGTCGGCGTCAGCAACCAGATTTTAAATTTAATTATCGTGATGTTCAGCTTTATCGCAACAGGAACGACCGTT
ATCATTTCACAATTTCTAGGCTCACGGCAAAAGAAAGAAGCCATGGAAGTCGCTTATGTGTCAATCGGCGCCAACTTCTT
CATCAGCCTGGCCATCAGCGCCGTCGTCTTTTTCGCGGCTGTCCCTCTGCTTCATATGATGGGATTATCAGACGCACTGA
TGCCTGATGCGAAAGTCTTTTTACAAGTTGTCGGAGGTCTGTCCTTTATTCAGGCTTTAATCATGACATTCAGCGCGATC
TTAAAAAGCTACGGCTACACAAAAGATACAATGTTTGTCACTATCGGCATGAACATTTTAAACATTGCCGGAAACTTTGT
TGTGATTTTCGGTTTGTTCGGCTTTCCTGTTCTCGGTGTTGCCGGTGTGGCCATGTCCACATCTATCGCCCGCGTCATCG
GATTAATCGCGATGATTGTGATTGTCAACAAACGCATCCAGCTGAAGATGTCGCTGAAAAAAGTCTTTCATATGCATAAA
GAGCATTTGCGCAAGCTGCTGAAAATCGGCATTCCTTCTGCGGGAGAGCAGCTTTCATACAACCTGTCGCAAATGATAGT
CACGTATTTTATCGCCATCATGGGCGCGCAGGCGTTGACAACAAAAGTGTATACGCAAAATATTACGATGTTTATTTTGC
TGTTCGGCACTGCGATCAGCCAGGGTACACAAATCTTAATCGGCCGTTATATCGGCGGCAAACAATTTGATGCCGCTTAT
GAGCGCTGCATGAAGAGCTTATATTGGGCACTCGGAATTGCTGCGGCTACCTCTGTTTTGATGACGATCTTTTCAAAAGA
CCTGATCGGACTCTTCACACAAAGCCCTGACATTATAGCAACTGCAAGCCTATTGATTGCCATGACCATTATCCTTGAAC
CAGGCCGCTCGTTTAATGTCATTATCATTAACTCTCTGCGGGCCGCGGGGGATGCGAAATTCCCGGTTTACATGGCAATG
ATTTCAATGTGGGGCATTGGCCTCCCGCTTGCTTATCTTTTCGGCATCCACCTCGGTTTCGGTCTTGCCGGCATTTGGAT
TTCTTTTATCGCGGATGAATGGGTCCGGGGAATCTTAATGTACAGAAGATGGCGCTCACGTATCTGGATTCAAAAAGGTA
TGGCGTAA

Upstream 100 bases:

>100_bases
CGGATTTTTACAAGAAGTATCGTTTCTATCCCTTTTTATTATAAGGAAATGGAGGTACACTTTAAAACGTTGATTTTATT
TACGAAAGGGGTTAGACACG

Downstream 100 bases:

>100_bases
CACCCGGCTCAATCAAGGCCGGGTGTTTTTTAGATATCAGACAGAATCTGCTTCACTTCGGTCTCTGTGAGAGCGAAGTG
ATATTGAAATACATCGTGCT

Product: Na+driven efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKQAVFKSTALKKPADKTFSLFSLTWPIFIEVSLYMFMGNADTLMLSQYSDNSVAAVGVSNQILNLIIVMFSFIATGTTV
IISQFLGSRQKKEAMEVAYVSIGANFFISLAISAVVFFAAVPLLHMMGLSDALMPDAKVFLQVVGGLSFIQALIMTFSAI
LKSYGYTKDTMFVTIGMNILNIAGNFVVIFGLFGFPVLGVAGVAMSTSIARVIGLIAMIVIVNKRIQLKMSLKKVFHMHK
EHLRKLLKIGIPSAGEQLSYNLSQMIVTYFIAIMGAQALTTKVYTQNITMFILLFGTAISQGTQILIGRYIGGKQFDAAY
ERCMKSLYWALGIAAATSVLMTIFSKDLIGLFTQSPDIIATASLLIAMTIILEPGRSFNVIIINSLRAAGDAKFPVYMAM
ISMWGIGLPLAYLFGIHLGFGLAGIWISFIADEWVRGILMYRRWRSRIWIQKGMA

Sequences:

>Translated_455_residues
MKQAVFKSTALKKPADKTFSLFSLTWPIFIEVSLYMFMGNADTLMLSQYSDNSVAAVGVSNQILNLIIVMFSFIATGTTV
IISQFLGSRQKKEAMEVAYVSIGANFFISLAISAVVFFAAVPLLHMMGLSDALMPDAKVFLQVVGGLSFIQALIMTFSAI
LKSYGYTKDTMFVTIGMNILNIAGNFVVIFGLFGFPVLGVAGVAMSTSIARVIGLIAMIVIVNKRIQLKMSLKKVFHMHK
EHLRKLLKIGIPSAGEQLSYNLSQMIVTYFIAIMGAQALTTKVYTQNITMFILLFGTAISQGTQILIGRYIGGKQFDAAY
ERCMKSLYWALGIAAATSVLMTIFSKDLIGLFTQSPDIIATASLLIAMTIILEPGRSFNVIIINSLRAAGDAKFPVYMAM
ISMWGIGLPLAYLFGIHLGFGLAGIWISFIADEWVRGILMYRRWRSRIWIQKGMA
>Mature_455_residues
MKQAVFKSTALKKPADKTFSLFSLTWPIFIEVSLYMFMGNADTLMLSQYSDNSVAAVGVSNQILNLIIVMFSFIATGTTV
IISQFLGSRQKKEAMEVAYVSIGANFFISLAISAVVFFAAVPLLHMMGLSDALMPDAKVFLQVVGGLSFIQALIMTFSAI
LKSYGYTKDTMFVTIGMNILNIAGNFVVIFGLFGFPVLGVAGVAMSTSIARVIGLIAMIVIVNKRIQLKMSLKKVFHMHK
EHLRKLLKIGIPSAGEQLSYNLSQMIVTYFIAIMGAQALTTKVYTQNITMFILLFGTAISQGTQILIGRYIGGKQFDAAY
ERCMKSLYWALGIAAATSVLMTIFSKDLIGLFTQSPDIIATASLLIAMTIILEPGRSFNVIIINSLRAAGDAKFPVYMAM
ISMWGIGLPLAYLFGIHLGFGLAGIWISFIADEWVRGILMYRRWRSRIWIQKGMA

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Escherichia coli, GI226510954, Length=442, Percent_Identity=26.9230769230769, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI48994890, Length=437, Percent_Identity=21.9679633867277, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YISQ_BACSU (O07940)

Other databases:

- EMBL:   Y09476
- EMBL:   Z93940
- EMBL:   AL009126
- PIR:   H69837
- RefSeq:   NP_388962.1
- ProteinModelPortal:   O07940
- EnsemblBacteria:   EBBACT00000000451
- GeneID:   939335
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10820
- NMPDR:   fig|224308.1.peg.1081
- GenoList:   BSU10820
- GeneTree:   EBGT00050000000379
- HOGENOM:   HBG531757
- OMA:   YDYLLIC
- PhylomeDB:   O07940
- ProtClustDB:   CLSK873468
- BioCyc:   BSUB:BSU10820-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 50013; Mature: 50013

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdaf9784)-; HASH(0xb4aa528)-; HASH(0xc693148)-; HASH(0xda26d64)-; HASH(0xe372484)-; HASH(0xe171754)-; HASH(0xe2ca424)-; HASH(0xe0cc6b0)-; HASH(0xd7d7338)-; HASH(0xde0ce50)-; HASH(0xe339adc)-; HASH(0xd991590)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAVFKSTALKKPADKTFSLFSLTWPIFIEVSLYMFMGNADTLMLSQYSDNSVAAVGVS
CCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHEEHHHEECCCCEEEEEECCCCCEEEECCH
NQILNLIIVMFSFIATGTTVIISQFLGSRQKKEAMEVAYVSIGANFFISLAISAVVFFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPLLHMMGLSDALMPDAKVFLQVVGGLSFIQALIMTFSAILKSYGYTKDTMFVTIGMNIL
HHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
NIAGNFVVIFGLFGFPVLGVAGVAMSTSIARVIGLIAMIVIVNKRIQLKMSLKKVFHMHK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EHLRKLLKIGIPSAGEQLSYNLSQMIVTYFIAIMGAQALTTKVYTQNITMFILLFGTAIS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
QGTQILIGRYIGGKQFDAAYERCMKSLYWALGIAAATSVLMTIFSKDLIGLFTQSPDIIA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TASLLIAMTIILEPGRSFNVIIINSLRAAGDAKFPVYMAMISMWGIGLPLAYLFGIHLGF
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
GLAGIWISFIADEWVRGILMYRRWRSRIWIQKGMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MKQAVFKSTALKKPADKTFSLFSLTWPIFIEVSLYMFMGNADTLMLSQYSDNSVAAVGVS
CCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHEEHHHEECCCCEEEEEECCCCCEEEECCH
NQILNLIIVMFSFIATGTTVIISQFLGSRQKKEAMEVAYVSIGANFFISLAISAVVFFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPLLHMMGLSDALMPDAKVFLQVVGGLSFIQALIMTFSAILKSYGYTKDTMFVTIGMNIL
HHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
NIAGNFVVIFGLFGFPVLGVAGVAMSTSIARVIGLIAMIVIVNKRIQLKMSLKKVFHMHK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EHLRKLLKIGIPSAGEQLSYNLSQMIVTYFIAIMGAQALTTKVYTQNITMFILLFGTAIS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
QGTQILIGRYIGGKQFDAAYERCMKSLYWALGIAAATSVLMTIFSKDLIGLFTQSPDIIA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TASLLIAMTIILEPGRSFNVIIINSLRAAGDAKFPVYMAMISMWGIGLPLAYLFGIHLGF
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
GLAGIWISFIADEWVRGILMYRRWRSRIWIQKGMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377