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Identifier: 16078032

GI number: 16078032

Start: 1041994

End: 1042842

Strand: Direct

Name: dat

Synonym: BSU09670

Alternate gene names: 16078032

Gene position: 1041994-1042842 (Clockwise)

Preceding gene: 16078030

Following gene: 16078035

Centisome position: 24.72

GC content: 47.35

Gene sequence:

>849_bases
ATGAAGGTTTTAGTCAATGGCCGGCTGATTGGGCGCAGTGAAGCATCAATCGATTTGGAAGATCGCGGTTATCAGTTTGG
TGACGGCATCTATGAAGTGATCAGGGTGTACAAAGGAGTATTGTTCGGCTTACGTGAGCATGCAGAGCGTTTTTTCAGAA
GTGCTGCTGAAATCGGAATTTCACTGCCATTCAGTATAGAAGATCTCGAGTGGGACCTGCAAAAGCTTGTACAGGAAAAT
GCGGTCAGTGAGGGAGCGGTATACATTCAGACAACAAGAGGTGTGGCCCCGCGAAAACACCAGTATGAAGCCGGCCTCGA
GCCGCAGACTACTGCCTATACGTTTACGGTGAAAAAACCGGAGCAAGAGCAGGCATACGGAGTGGCGGCCATTACAGATG
AGGATCTTCGCTGGTTAAGATGTGATATCAAAAGTCTGAATTTACTGTATAATGTCATGACGAAGCAAAGGGCCTATGAA
GCCGGAGCATTTGAAGCCATTTTACTTAGGGACGGCGTTGTTACGGAGGGTACATCCTCTAACGTTTATGCCGTTATCAA
CGGCACAGTGCGAACACATCCGGCTAATCGGCTCATTCTCAATGGAATTACACGGATGAATATTTTAGGACTGATTGAGA
AGAATGGGATCAAACTGGATGAGACTCCTGTCAGTGAAGAAGAGTTGAAACAGGCGGAAGAGATCTTTATTTCGTCAACG
ACGGCAGAAATTATTCCGGTCGTGACGCTCGATGGACAATCGATCGGAAGCGGGAAACCCGGACCGGTGACCAAACAGCT
TCAGGCTGCTTTTCAAGAAAGCATTCAACAGGCTGCTAGCATTTCATAA

Upstream 100 bases:

>100_bases
TCTCTTGATCTTTCTGTTTTTCAGCTTGTCCAATATGGTAGAATTAGTGATAATGACAGCTTGAATGACATCAGGGACAT
CTCGAAAAGGGAGGGCTTTT

Downstream 100 bases:

>100_bases
ATGAACGAAGAAAAGAGCCCGCTTTTTACAGCGGGGCTCTTTTTATTTTTTAACAAATCCAATCAAAATGGAGATCATTG
GCGATATATAGAGGAAGAAC

Product: D-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKVLVNGRLIGRSEASIDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKLVQEN
AVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKPEQEQAYGVAAITDEDLRWLRCDIKSLNLLYNVMTKQRAYE
AGAFEAILLRDGVVTEGTSSNVYAVINGTVRTHPANRLILNGITRMNILGLIEKNGIKLDETPVSEEELKQAEEIFISST
TAEIIPVVTLDGQSIGSGKPGPVTKQLQAAFQESIQQAASIS

Sequences:

>Translated_282_residues
MKVLVNGRLIGRSEASIDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKLVQEN
AVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKPEQEQAYGVAAITDEDLRWLRCDIKSLNLLYNVMTKQRAYE
AGAFEAILLRDGVVTEGTSSNVYAVINGTVRTHPANRLILNGITRMNILGLIEKNGIKLDETPVSEEELKQAEEIFISST
TAEIIPVVTLDGQSIGSGKPGPVTKQLQAAFQESIQQAASIS
>Mature_282_residues
MKVLVNGRLIGRSEASIDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKLVQEN
AVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKPEQEQAYGVAAITDEDLRWLRCDIKSLNLLYNVMTKQRAYE
AGAFEAILLRDGVVTEGTSSNVYAVINGTVRTHPANRLILNGITRMNILGLIEKNGIKLDETPVSEEELKQAEEIFISST
TAEIIPVVTLDGQSIGSGKPGPVTKQLQAAFQESIQQAASIS

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Escherichia coli, GI48994963, Length=287, Percent_Identity=28.9198606271777, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787338, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DAAA_BACSU (O07597)

Other databases:

- EMBL:   Y14082
- EMBL:   Y14080
- EMBL:   AL009126
- PIR:   E69829
- RefSeq:   NP_388848.1
- ProteinModelPortal:   O07597
- SMR:   O07597
- EnsemblBacteria:   EBBACT00000003373
- GeneID:   939759
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09670
- NMPDR:   fig|224308.1.peg.967
- GenoList:   BSU09670
- GeneTree:   EBGT00070000032040
- HOGENOM:   HBG617123
- OMA:   RDGWLTE
- PhylomeDB:   O07597
- ProtClustDB:   CLSK886974
- BioCyc:   BSUB:BSU09670-MONOMER
- BRENDA:   2.6.1.21
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784
- PANTHER:   PTHR11825
- TIGRFAMs:   TIGR01121

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.21

Molecular weight: Translated: 31183; Mature: 31183

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: ACT_SITE 144-144 BINDING 31-31 BINDING 50-50 BINDING 98-98 BINDING 100-100 BINDING 176-176 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVNGRLIGRSEASIDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGI
CEEEEECEEEECCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
SLPFSIEDLEWDLQKLVQENAVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKP
ECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCC
EQEQAYGVAAITDEDLRWLRCDIKSLNLLYNVMTKQRAYEAGAFEAILLRDGVVTEGTSS
CHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCC
NVYAVINGTVRTHPANRLILNGITRMNILGLIEKNGIKLDETPVSEEELKQAEEIFISST
CEEEEEECEEEECCCCCHHHHCCHHHHHEEEEECCCEECCCCCCCHHHHHHHHHHHHCCC
TAEIIPVVTLDGQSIGSGKPGPVTKQLQAAFQESIQQAASIS
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLVNGRLIGRSEASIDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGI
CEEEEECEEEECCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
SLPFSIEDLEWDLQKLVQENAVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKP
ECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCC
EQEQAYGVAAITDEDLRWLRCDIKSLNLLYNVMTKQRAYEAGAFEAILLRDGVVTEGTSS
CHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCC
NVYAVINGTVRTHPANRLILNGITRMNILGLIEKNGIKLDETPVSEEELKQAEEIFISST
CEEEEEECEEEECCCCCHHHHCCHHHHHEEEEECCCEECCCCCCCHHHHHHHHHHHHCCC
TAEIIPVVTLDGQSIGSGKPGPVTKQLQAAFQESIQQAASIS
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377