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The map label for this gene is yhcA

Identifier: 16077966

GI number: 16077966

Start: 977775

End: 979373

Strand: Direct

Name: yhcA

Synonym: BSU09010

Alternate gene names: 16077966

Gene position: 977775-979373 (Clockwise)

Preceding gene: 16077965

Following gene: 16077967

Centisome position: 23.19

GC content: 43.84

Gene sequence:

>1599_bases
ATGGCTACAGGCAAAGAAAAAAATGCAAAAAACCCTATGTCATTGCTGATTGTGCTAATGGCGGGTTTATTTTTGGCAAT
TCTAAACCAAACACTGCTTAATGTTGCAATGCCGCACTTAATGACTGAATTTGGCGTAAGTGCAACGACGATTCAGTGGC
TGACAACAGGTTACATGCTTGTTAACGGTGTATTGATTCCGTTATCTGCTTTCCTGATTACACGATTTGGCCAGCGGAGT
CTCTTTCTCGTCGCGATGTTTTGTTTTACGCTCGGTACGTTAGTCTGCGGTATCGCACCTAACTTCTCTACAATGCTGAT
CGGCCGTTTGATTCAGGCCGTGGGCGGAGGTATCTTGCAGCCGCTCGTTATGACAACCATTCTGTTAATTTTCCCGCCTG
AAAGCAGGGGGAAAGGAATGGGGATTTTCGGGCTAGCGATGATGTTTGCTCCAGCGGTCGGCCCGACTCTTTCCGGGTGG
ATTATTGAGCATTATACATGGCGCATTATGTTTTACGGACTTGTGCCGATCGGCGCAATTGTTATCATTGTGGCTTTCTT
CATCTTTAAAAATATGGTGGAGCCGCAAAAAATCAAACTCGATACTCTTGGCGCAATCCTCTCGATTGTCGGATTCGCCT
CCTTATTATACGGAGTCAGTGAAGCCGGATCTGACGGCTGGACCGACCCGATTGTATTGTCTACAGTTATTATCGGAGCC
ATTGCAATTGTTGCTTTTGTTGTTCAGCAATTGCGTCATGATGATCCGATGCTTGATTTCAGAGTGTTTAAATATGACAT
TTTCTCACTCTCGAGTGTCATTAATATCATTATTACAGTGGCACTTTACACTGGTATGTTCCTTTTACCGATTTATCTGC
AAAACCTGGTCGGCTTTACGGCGCTTCAATCCGGTCTGCTTCTGCTTCCGGGTGCGATCGTTATGCTGATTATGTCACCG
ATCTCAGGTATTTTGTTCGATAAATTCGGACCGAGACCGCTTGCGATTATCGGGCTTCTCGTGACAGTGGTAACAACGTA
TCAATACACGCAATTGACAATTGATACACCATATACACACATCATGCTGATCTATTCAATTCGTGCATTTGGTATGTCTC
TCTTAATGATGCCAGTCATGACAGCCGGGATGAACCAGCTTCCTGCCCGTCTGAACAGCCACGGAACGGCGATGAGTAAC
ACGCTTCGCCAAATCAGCGGATCAATCGGAACAAGTTTAATTACGACGATTTATACAAACCGGACGACATTCCATTATTC
ACAAATTGCAGATAAAACAAGCACGGCGGACCCGAACTTCCTCCATGCGTTCCAAAATGCAGTTTCGAATTTAATGGTGA
ATATGAATGTGTCTTATGATACGGCGAAAACATATGTGTACTCACATATTTATAAACACGCATCACTCGATTCCAATGTC
ATGGGGATAAATGATGCCTTTATGTGGGCAACCTTATTCTGTGTAGCAGGTTTGATTTTAAGTATCTTCCTGCGTGACGT
CAGAAAAGATAAATTGCGTAAGAAGAAAAAAGAGGAGCTTTCATTACTTCCTGCTCCAAAAGAAGCTAAGGAATCTTAA

Upstream 100 bases:

>100_bases
AAAAACCCATCTGATAAGGTGCTTCCTGGAATGAATGCATCTGTTAAAATTTCTGAGTAAAACAGATCAGTCATCAAACC
ATGAAAGGAGGTTGTCTTAG

Downstream 100 bases:

>100_bases
TCTGTACAGGGAGGTTTCCTGAATGAAAATTTATGTAGTGTATGATAGTGAAGGCGAACATACTAAAGTGCTTGCAGAAG
CGATTGCTGAAGGCGCGAGA

Product: exporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MATGKEKNAKNPMSLLIVLMAGLFLAILNQTLLNVAMPHLMTEFGVSATTIQWLTTGYMLVNGVLIPLSAFLITRFGQRS
LFLVAMFCFTLGTLVCGIAPNFSTMLIGRLIQAVGGGILQPLVMTTILLIFPPESRGKGMGIFGLAMMFAPAVGPTLSGW
IIEHYTWRIMFYGLVPIGAIVIIVAFFIFKNMVEPQKIKLDTLGAILSIVGFASLLYGVSEAGSDGWTDPIVLSTVIIGA
IAIVAFVVQQLRHDDPMLDFRVFKYDIFSLSSVINIIITVALYTGMFLLPIYLQNLVGFTALQSGLLLLPGAIVMLIMSP
ISGILFDKFGPRPLAIIGLLVTVVTTYQYTQLTIDTPYTHIMLIYSIRAFGMSLLMMPVMTAGMNQLPARLNSHGTAMSN
TLRQISGSIGTSLITTIYTNRTTFHYSQIADKTSTADPNFLHAFQNAVSNLMVNMNVSYDTAKTYVYSHIYKHASLDSNV
MGINDAFMWATLFCVAGLILSIFLRDVRKDKLRKKKKEELSLLPAPKEAKES

Sequences:

>Translated_532_residues
MATGKEKNAKNPMSLLIVLMAGLFLAILNQTLLNVAMPHLMTEFGVSATTIQWLTTGYMLVNGVLIPLSAFLITRFGQRS
LFLVAMFCFTLGTLVCGIAPNFSTMLIGRLIQAVGGGILQPLVMTTILLIFPPESRGKGMGIFGLAMMFAPAVGPTLSGW
IIEHYTWRIMFYGLVPIGAIVIIVAFFIFKNMVEPQKIKLDTLGAILSIVGFASLLYGVSEAGSDGWTDPIVLSTVIIGA
IAIVAFVVQQLRHDDPMLDFRVFKYDIFSLSSVINIIITVALYTGMFLLPIYLQNLVGFTALQSGLLLLPGAIVMLIMSP
ISGILFDKFGPRPLAIIGLLVTVVTTYQYTQLTIDTPYTHIMLIYSIRAFGMSLLMMPVMTAGMNQLPARLNSHGTAMSN
TLRQISGSIGTSLITTIYTNRTTFHYSQIADKTSTADPNFLHAFQNAVSNLMVNMNVSYDTAKTYVYSHIYKHASLDSNV
MGINDAFMWATLFCVAGLILSIFLRDVRKDKLRKKKKEELSLLPAPKEAKES
>Mature_531_residues
ATGKEKNAKNPMSLLIVLMAGLFLAILNQTLLNVAMPHLMTEFGVSATTIQWLTTGYMLVNGVLIPLSAFLITRFGQRSL
FLVAMFCFTLGTLVCGIAPNFSTMLIGRLIQAVGGGILQPLVMTTILLIFPPESRGKGMGIFGLAMMFAPAVGPTLSGWI
IEHYTWRIMFYGLVPIGAIVIIVAFFIFKNMVEPQKIKLDTLGAILSIVGFASLLYGVSEAGSDGWTDPIVLSTVIIGAI
AIVAFVVQQLRHDDPMLDFRVFKYDIFSLSSVINIIITVALYTGMFLLPIYLQNLVGFTALQSGLLLLPGAIVMLIMSPI
SGILFDKFGPRPLAIIGLLVTVVTTYQYTQLTIDTPYTHIMLIYSIRAFGMSLLMMPVMTAGMNQLPARLNSHGTAMSNT
LRQISGSIGTSLITTIYTNRTTFHYSQIADKTSTADPNFLHAFQNAVSNLMVNMNVSYDTAKTYVYSHIYKHASLDSNVM
GINDAFMWATLFCVAGLILSIFLRDVRKDKLRKKKKEELSLLPAPKEAKES

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1789042, Length=428, Percent_Identity=28.2710280373832, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788710, Length=436, Percent_Identity=28.4403669724771, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87081983, Length=395, Percent_Identity=24.3037974683544, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790195, Length=449, Percent_Identity=25.8351893095768, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788392, Length=444, Percent_Identity=25.4504504504505, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323735, Length=323, Percent_Identity=26.625386996904, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=250, Percent_Identity=26.4, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=503, Percent_Identity=21.8687872763419, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=30.2013422818792, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=309, Percent_Identity=27.1844660194175, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHCA_BACSU (P54585)

Other databases:

- EMBL:   AL009126
- EMBL:   X96983
- PIR:   E69821
- RefSeq:   NP_388782.1
- ProteinModelPortal:   P54585
- SMR:   P54585
- EnsemblBacteria:   EBBACT00000004004
- GeneID:   936235
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09010
- NMPDR:   fig|224308.1.peg.901
- GenoList:   BSU09010
- GeneTree:   EBGT00070000031768
- HOGENOM:   HBG679341
- OMA:   VMGINDA
- PhylomeDB:   P54585
- ProtClustDB:   CLSK886927
- BioCyc:   BSUB:BSU09010-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 58343; Mature: 58211

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xddf482c)-; HASH(0xd8a8cf8)-; HASH(0xdeb78d8)-; HASH(0xd81d490)-; HASH(0xd990044)-; HASH(0xdeb0558)-; HASH(0xd9b5044)-; HASH(0xd68a040)-; HASH(0xde0beec)-; HASH(0xdea8f60)-; HASH(0xd9a3bd0)-; HASH(0xd973cd0)-; HASH(0xd81d514)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
>Mature Secondary Structure 
ATGKEKNAKNPMSLLIVLMAGLFLAILNQTLLNVAMPHLMTEFGVSATTIQWLTTGYML
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498