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The map label for this gene is yfiK

Identifier: 16077897

GI number: 16077897

Start: 905010

End: 905672

Strand: Direct

Name: yfiK

Synonym: BSU08300

Alternate gene names: 16077897

Gene position: 905010-905672 (Clockwise)

Preceding gene: 16077896

Following gene: 255767193

Centisome position: 21.47

GC content: 48.11

Gene sequence:

>663_bases
ATGATTAAGATCATCATTACCGACGATCAGGATATCGTCAGAGAAGGGCTGGCATCCCTGCTCCAGCTCCGAGAAGAGCT
CGATGTGATCGCAACGGCGCGAAACGGACAGGAAGCCTTCGAAAAGGCGAAAGAGCTGGAGCCGGATATCGTGTTAATGG
ACATCCGCATGCCGGTTTCCAACGGGGTGGAGGGAACAAAATTAATCACAAGCTCGCTGCCAAGCGTAAAGGTTTTGATG
CTGACAACCTTTAAGGATTCAGCGCTGATTGCTGAAGCGCTTGAAGAGGGAGCAAGCGGGTATCTGCTGAAGGATATGTC
TGCAGATACGATTGTAAAAGCAGTGATGACTGTTCATTCTGGCGGAATGGTGCTTCCGCCGGAATTGACTGCCCAAATGC
TGAATGAATGGAAGCGTGAAAAACAGCTGAAAGGAATAAATGAGATAGAAAAACCTAACGAGCTGCTCGACCTGACCGAG
CGCGAAATCGAAGTTCTGGCTGAGCTGGGATACGGGCTGAATAACAAAGAAATTGCCGAAAAGCTTTACATTACAGAAGG
CACAGTCAAAAACCACGTTTCAAATATTATCAGCAAGCTGGCTGTCAGGGACAGAACCCAAGCCGCCATTTACTCGGTCA
GATATGGTGTATCTGTCTTTTGA

Upstream 100 bases:

>100_bases
TTGAAAGCGAACGGGATCAAGGATTTACTGTAAATGCAGAATTTTCACTTGCCAATAAAAAATGGAGCTTCGGGCCCGTG
CAGCAAAAGGAGAGTTTATC

Downstream 100 bases:

>100_bases
CATATGACGTTTTGCATATACGCTTCTCTGAAAAGGCCTTTTACAGGCCTTTTTTTCATGCCCTATGTTCTATCTGCCGC
TACATGCCGCACGCCTTTCT

Product: two-component response regulator YfiJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MIKIIITDDQDIVREGLASLLQLREELDVIATARNGQEAFEKAKELEPDIVLMDIRMPVSNGVEGTKLITSSLPSVKVLM
LTTFKDSALIAEALEEGASGYLLKDMSADTIVKAVMTVHSGGMVLPPELTAQMLNEWKREKQLKGINEIEKPNELLDLTE
REIEVLAELGYGLNNKEIAEKLYITEGTVKNHVSNIISKLAVRDRTQAAIYSVRYGVSVF

Sequences:

>Translated_220_residues
MIKIIITDDQDIVREGLASLLQLREELDVIATARNGQEAFEKAKELEPDIVLMDIRMPVSNGVEGTKLITSSLPSVKVLM
LTTFKDSALIAEALEEGASGYLLKDMSADTIVKAVMTVHSGGMVLPPELTAQMLNEWKREKQLKGINEIEKPNELLDLTE
REIEVLAELGYGLNNKEIAEKLYITEGTVKNHVSNIISKLAVRDRTQAAIYSVRYGVSVF
>Mature_220_residues
MIKIIITDDQDIVREGLASLLQLREELDVIATARNGQEAFEKAKELEPDIVLMDIRMPVSNGVEGTKLITSSLPSVKVLM
LTTFKDSALIAEALEEGASGYLLKDMSADTIVKAVMTVHSGGMVLPPELTAQMLNEWKREKQLKGINEIEKPNELLDLTE
REIEVLAELGYGLNNKEIAEKLYITEGTVKNHVSNIISKLAVRDRTQAAIYSVRYGVSVF

Specific function: Member of the two-component regulatory system yfiJ/yfiK

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=33.4928229665072, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788222, Length=219, Percent_Identity=29.6803652968037, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1790102, Length=210, Percent_Identity=32.3809523809524, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787473, Length=209, Percent_Identity=33.9712918660287, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786747, Length=212, Percent_Identity=29.7169811320755, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788712, Length=199, Percent_Identity=23.6180904522613, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789937, Length=211, Percent_Identity=27.4881516587678, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=33.6448598130841, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=32.6923076923077, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=109, Percent_Identity=35.7798165137615, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFIK_BACSU (P94439)

Other databases:

- EMBL:   D78508
- EMBL:   AL009126
- PIR:   JC5359
- RefSeq:   NP_388711.1
- HSSP:   P11470
- ProteinModelPortal:   P94439
- SMR:   P94439
- EnsemblBacteria:   EBBACT00000000799
- GeneID:   939209
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08300
- NMPDR:   fig|224308.1.peg.830
- GenoList:   BSU08300
- GeneTree:   EBGT00070000031976
- HOGENOM:   HBG753323
- OMA:   MSADTIV
- PhylomeDB:   P94439
- ProtClustDB:   CLSK886892
- BioCyc:   BSUB:BSU08300-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 24372; Mature: 24372

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIIITDDQDIVREGLASLLQLREELDVIATARNGQEAFEKAKELEPDIVLMDIRMPVS
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCC
NGVEGTKLITSSLPSVKVLMLTTFKDSALIAEALEEGASGYLLKDMSADTIVKAVMTVHS
CCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCC
GGMVLPPELTAQMLNEWKREKQLKGINEIEKPNELLDLTEREIEVLAELGYGLNNKEIAE
CCEEECHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KLYITEGTVKNHVSNIISKLAVRDRTQAAIYSVRYGVSVF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKIIITDDQDIVREGLASLLQLREELDVIATARNGQEAFEKAKELEPDIVLMDIRMPVS
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCC
NGVEGTKLITSSLPSVKVLMLTTFKDSALIAEALEEGASGYLLKDMSADTIVKAVMTVHS
CCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCC
GGMVLPPELTAQMLNEWKREKQLKGINEIEKPNELLDLTEREIEVLAELGYGLNNKEIAE
CCEEECHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KLYITEGTVKNHVSNIISKLAVRDRTQAAIYSVRYGVSVF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377