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The map label for this gene is yfiJ

Identifier: 16077896

GI number: 16077896

Start: 903811

End: 905013

Strand: Direct

Name: yfiJ

Synonym: BSU08290

Alternate gene names: 16077896

Gene position: 903811-905013 (Clockwise)

Preceding gene: 16077895

Following gene: 16077897

Centisome position: 21.44

GC content: 47.38

Gene sequence:

>1203_bases
GTGAAAGCTCTTTTTTTTACGAGAATGTTCACCTTGATGGTGAGCTGTTTAATGTATCTGTCCATTGTGAAAGAGGATAA
CTGGTTTGGATATGTATTTATAGCAGCAGGTGCTGCCATGTATGCAGCCAATCATGTTTTGCTGACAAAAGAGACAAATG
CCATCTGGTTCTGCCTGATTGATATTGCAATCGGCTTTTCGTTCGGATTTATCTTTCCCGGCACAGGTTTGTTCATTATT
ATGCTTTGTCCGGTTGCGGTCGCCTTTTTTCTGCGGGGGTTTCCTAAAAGAACAGCATGGTCTGTCTTATGTCTTTCCTC
TATTTTATTTTTGACCGTCCTCATCCGTACATATGCGATGTTCGGCAATGAATTTGTCATTGATCATTTGACTAGCATGA
CATTCGTTGTCTTCTGCGGTGTCGTGGGCAAATTAATCCGCAAGCTGTTGGATGCGCAGGATACGGCAAAACAGCAGTTT
CAGGAACTGACGGAGTCCCATCTGGCGCTGTCTGCTGCACATCAGGAGCTCCATTTATATGCGAAGCAGGTTGAAGAGCT
GACTGCCATTTACGAGCGGAACAGAATGGCGCGTGAAATCCATGATACAGTGGGGCATAAAATGACGGCCCTTCTCGTCC
AGCTGCAGCTTTTGCGTGAATGGCAAAAAAGAGACAGCCAAAAAGCGGATGAGACAGTAGGTGTCTGTGAAACACTTGCC
CGTGAAGCGCTTGATGATGTCCGCTTATCAGTACGCACTCTGCAAACTGAAAACGATCCTTCGCTAATCGAATCGCTGAA
ACAGCTGACAGAGGACTTTTGCAAAAATGCAGGAGTCACAACAGAATTTGCGGTCAGCGGTGATCCTGCAATCATCCCGC
TGTCTCTCCATCCGACCCTGATCAGAACGGTGCAGGAAGCACTGACCAATGCCAAACGGCACGGCGGCGCCGCAGCCTGT
TCGATACAGCTTGCCTGCACAACGGACAGCATCAGCCTTGTCATTAAGGATGACGGCAAAGGCAATCCTGAGGCAGCACT
TGGTTTTGGTCTCTTGAATATGAAAAAGCGGGCAGCGGAGCATGGCGGAATGATCCGCTTTGAAAGCGAACGGGATCAAG
GATTTACTGTAAATGCAGAATTTTCACTTGCCAATAAAAAATGGAGCTTCGGGCCCGTGCAGCAAAAGGAGAGTTTATCA
TGA

Upstream 100 bases:

>100_bases
CATAAAGTTCATATGCGGTTTTTATTTTCCAGAAACTCCTGTCAAAAATGCCCTGAGGCAGGTATGATAAGAAGGGAATT
GGGTATCGGGAGTGATGAGC

Downstream 100 bases:

>100_bases
TTAAGATCATCATTACCGACGATCAGGATATCGTCAGAGAAGGGCTGGCATCCCTGCTCCAGCTCCGAGAAGAGCTCGAT
GTGATCGCAACGGCGCGAAA

Product: two-component sensor histidine kinase YfiK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKALFFTRMFTLMVSCLMYLSIVKEDNWFGYVFIAAGAAMYAANHVLLTKETNAIWFCLIDIAIGFSFGFIFPGTGLFII
MLCPVAVAFFLRGFPKRTAWSVLCLSSILFLTVLIRTYAMFGNEFVIDHLTSMTFVVFCGVVGKLIRKLLDAQDTAKQQF
QELTESHLALSAAHQELHLYAKQVEELTAIYERNRMAREIHDTVGHKMTALLVQLQLLREWQKRDSQKADETVGVCETLA
REALDDVRLSVRTLQTENDPSLIESLKQLTEDFCKNAGVTTEFAVSGDPAIIPLSLHPTLIRTVQEALTNAKRHGGAAAC
SIQLACTTDSISLVIKDDGKGNPEAALGFGLLNMKKRAAEHGGMIRFESERDQGFTVNAEFSLANKKWSFGPVQQKESLS

Sequences:

>Translated_400_residues
MKALFFTRMFTLMVSCLMYLSIVKEDNWFGYVFIAAGAAMYAANHVLLTKETNAIWFCLIDIAIGFSFGFIFPGTGLFII
MLCPVAVAFFLRGFPKRTAWSVLCLSSILFLTVLIRTYAMFGNEFVIDHLTSMTFVVFCGVVGKLIRKLLDAQDTAKQQF
QELTESHLALSAAHQELHLYAKQVEELTAIYERNRMAREIHDTVGHKMTALLVQLQLLREWQKRDSQKADETVGVCETLA
REALDDVRLSVRTLQTENDPSLIESLKQLTEDFCKNAGVTTEFAVSGDPAIIPLSLHPTLIRTVQEALTNAKRHGGAAAC
SIQLACTTDSISLVIKDDGKGNPEAALGFGLLNMKKRAAEHGGMIRFESERDQGFTVNAEFSLANKKWSFGPVQQKESLS
>Mature_400_residues
MKALFFTRMFTLMVSCLMYLSIVKEDNWFGYVFIAAGAAMYAANHVLLTKETNAIWFCLIDIAIGFSFGFIFPGTGLFII
MLCPVAVAFFLRGFPKRTAWSVLCLSSILFLTVLIRTYAMFGNEFVIDHLTSMTFVVFCGVVGKLIRKLLDAQDTAKQQF
QELTESHLALSAAHQELHLYAKQVEELTAIYERNRMAREIHDTVGHKMTALLVQLQLLREWQKRDSQKADETVGVCETLA
REALDDVRLSVRTLQTENDPSLIESLKQLTEDFCKNAGVTTEFAVSGDPAIIPLSLHPTLIRTVQEALTNAKRHGGAAAC
SIQLACTTDSISLVIKDDGKGNPEAALGFGLLNMKKRAAEHGGMIRFESERDQGFTVNAEFSLANKKWSFGPVQQKESLS

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI87082311, Length=213, Percent_Identity=27.2300469483568, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787474, Length=209, Percent_Identity=22.0095693779904, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFIJ_BACSU (P94438)

Other databases:

- EMBL:   D78508
- EMBL:   AL009126
- PIR:   JC5358
- RefSeq:   NP_388710.1
- ProteinModelPortal:   P94438
- SMR:   P94438
- EnsemblBacteria:   EBBACT00000002468
- GeneID:   936171
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08290
- NMPDR:   fig|224308.1.peg.829
- GenoList:   BSU08290
- GeneTree:   EBGT00070000031823
- HOGENOM:   HBG343340
- OMA:   LTNAKRH
- ProtClustDB:   CLSK886891
- BioCyc:   BSUB:BSU08290-MONOMER
- InterPro:   IPR003594
- InterPro:   IPR011712
- Gene3D:   G3DSA:3.30.565.10
- SMART:   SM00387

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3; SSF55874 ATP_bd_ATPase

EC number: =2.7.13.3

Molecular weight: Translated: 44543; Mature: 44543

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd37e474)-; HASH(0xd706a4c)-; HASH(0xcec6eac)-; HASH(0xd90bae8)-; HASH(0xcea4a6c)-;

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALFFTRMFTLMVSCLMYLSIVKEDNWFGYVFIAAGAAMYAANHVLLTKETNAIWFCLI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCEEEEEECCCEEEHHHH
DIAIGFSFGFIFPGTGLFIIMLCPVAVAFFLRGFPKRTAWSVLCLSSILFLTVLIRTYAM
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FGNEFVIDHLTSMTFVVFCGVVGKLIRKLLDAQDTAKQQFQELTESHLALSAAHQELHLY
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKQVEELTAIYERNRMAREIHDTVGHKMTALLVQLQLLREWQKRDSQKADETVGVCETLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
REALDDVRLSVRTLQTENDPSLIESLKQLTEDFCKNAGVTTEFAVSGDPAIIPLSLHPTL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCHHH
IRTVQEALTNAKRHGGAAACSIQLACTTDSISLVIKDDGKGNPEAALGFGLLNMKKRAAE
HHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
HGGMIRFESERDQGFTVNAEFSLANKKWSFGPVQQKESLS
CCCEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MKALFFTRMFTLMVSCLMYLSIVKEDNWFGYVFIAAGAAMYAANHVLLTKETNAIWFCLI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCEEEEEECCCEEEHHHH
DIAIGFSFGFIFPGTGLFIIMLCPVAVAFFLRGFPKRTAWSVLCLSSILFLTVLIRTYAM
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FGNEFVIDHLTSMTFVVFCGVVGKLIRKLLDAQDTAKQQFQELTESHLALSAAHQELHLY
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKQVEELTAIYERNRMAREIHDTVGHKMTALLVQLQLLREWQKRDSQKADETVGVCETLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
REALDDVRLSVRTLQTENDPSLIESLKQLTEDFCKNAGVTTEFAVSGDPAIIPLSLHPTL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCHHH
IRTVQEALTNAKRHGGAAACSIQLACTTDSISLVIKDDGKGNPEAALGFGLLNMKKRAAE
HHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
HGGMIRFESERDQGFTVNAEFSLANKKWSFGPVQQKESLS
CCCEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377