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The map label for this gene is dusC

Identifier: 16077870

GI number: 16077870

Start: 876426

End: 877403

Strand: Direct

Name: dusC

Synonym: BSU08030

Alternate gene names: 16077870

Gene position: 876426-877403 (Clockwise)

Preceding gene: 255767191

Following gene: 16077871

Centisome position: 20.79

GC content: 45.5

Gene sequence:

>978_bases
ATGACAGAAAATTTCTGGCGTGAATTACCACGGCCGTTTTTTGTACTGGCACCAATGGAAGATGTGACAGATGTTGTTTT
CCGGCATGTAGTAAGTGAAGCAGGCAGACCGGATGTGTTTTTTACAGAGTTCACAAACTCAGAGAGCTATTGCCACCCGG
ATGGGAAGGATAGCGTGCGCGGCCGTTTGACTTTTACAGAGGATGAACAGCCAATTGTGGCCCATATATGGGGAGATAAG
CCTGAAAACTTCCGTAAAATGAGTATTGGCATGGCAGAGTTAGGATTTAAAGGACTAGATATCAATATGGGCTGCCCTGT
GCCTAATGTGGCGGGGAATGGAAAGGGAAGCGGCCTTATTTGCCGTCCTGCAGTTGCGGCAGAATTAATACAAGCTGCAA
AAGCAGGGGGATTGCCTGTAAGCGTGAAGACAAGACTTGGATACACGGATGTGGATGAATGGCGCGAGTGGCTGACACAC
ATTTTGAAACAGGATATTGCGAATCTTTCTATTCATCTGCGTACAAGGGCAGAAATGAGCAAAGTAGATGCGCATTGGGA
GCTCATCCCAGAGATTAAGAAACTTCGTGACGAGGTGGCACCAGATACATTGCTAACAATAAACGGGGATATTCCTGACC
GTCAAACTGGCTTGAAACTTGCTGAACAATACGGCGTCGATGGAATTATGATAGGGCGCGGTATTTTTACAAATCCTTTC
GCTTTTGAAAAGGAGCCGAAAGAGCATAGCAGCAAGGAATTGCTTGATCTCTTAAGGCTGCATCTGGATCTCCATGATGA
ATATTCAAAAGAAGAAGCCCGTCCATACAAGCCTCTTCCTCGCTTTTTCAAGATTTATCTCCGCGGATTCCGAGGGGCAA
GTGAATTAAGAAATCAATGCATGAACACAAAGTCAACAGATGAAGTGCGTGCATTGCTCGACGACTTTGAGAGAAAGTAT
CTTGATGGGATAGAGTAA

Upstream 100 bases:

>100_bases
AATGCTTTCGCATGCGATAAGTGTAAAAGAAGCATGGTCAGTTTTTAATTTGGGATAAAGGTTCAGCCGTATCCCATAAT
GAAAGGATTTGATTTTTGTT

Downstream 100 bases:

>100_bases
CAGCAGTGCTTGATTCAAAAAAGGCTAACAAAATAAAGGGATTTTTACACAATTCTTGACTAAAATAAACCTGGATTTTC
GGTAAATCCGGGTCTTTTTT

Product: tRNA-dihydrouridine synthase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSESYCHPDGKDSVRGRLTFTEDEQPIVAHIWGDK
PENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLICRPAVAAELIQAAKAGGLPVSVKTRLGYTDVDEWREWLTH
ILKQDIANLSIHLRTRAEMSKVDAHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPF
AFEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQCMNTKSTDEVRALLDDFERKY
LDGIE

Sequences:

>Translated_325_residues
MTENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSESYCHPDGKDSVRGRLTFTEDEQPIVAHIWGDK
PENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLICRPAVAAELIQAAKAGGLPVSVKTRLGYTDVDEWREWLTH
ILKQDIANLSIHLRTRAEMSKVDAHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPF
AFEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQCMNTKSTDEVRALLDDFERKY
LDGIE
>Mature_324_residues
TENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSESYCHPDGKDSVRGRLTFTEDEQPIVAHIWGDKP
ENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLICRPAVAAELIQAAKAGGLPVSVKTRLGYTDVDEWREWLTHI
LKQDIANLSIHLRTRAEMSKVDAHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPFA
FEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQCMNTKSTDEVRALLDDFERKYL
DGIE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family

Homologues:

Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=27.5, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789660, Length=292, Percent_Identity=27.7397260273973, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI145693211, Length=238, Percent_Identity=26.0504201680672, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=232, Percent_Identity=27.1551724137931, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=260, Percent_Identity=28.4615384615385, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=314, Percent_Identity=24.203821656051, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=25.8474576271186, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUS2_BACSU (O31546)

Other databases:

- EMBL:   D78509
- EMBL:   AL009126
- PIR:   E69806
- RefSeq:   NP_388684.1
- ProteinModelPortal:   O31546
- SMR:   O31546
- EnsemblBacteria:   EBBACT00000001715
- GeneID:   936154
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08030
- NMPDR:   fig|224308.1.peg.803
- GenoList:   BSU08030
- GeneTree:   EBGT00050000000511
- HOGENOM:   HBG352957
- OMA:   VVFRHVV
- PhylomeDB:   O31546
- ProtClustDB:   CLSK865525
- BioCyc:   BSUB:BSU08030-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 36991; Mature: 36859

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSESYCHPDGKDSVR
CCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
GRLTFTEDEQPIVAHIWGDKPENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLI
EEEEECCCCCCEEEEECCCCCCCHHHHHCCHHHHCCCCCEEECCCCCCCCCCCCCCCCEE
CRPAVAAELIQAAKAGGLPVSVKTRLGYTDVDEWREWLTHILKQDIANLSIHLRTRAEMS
ECHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEHHHHH
KVDAHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQC
CCCCCCHHCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHH
MNTKSTDEVRALLDDFERKYLDGIE
CCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSESYCHPDGKDSVR
CCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
GRLTFTEDEQPIVAHIWGDKPENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLI
EEEEECCCCCCEEEEECCCCCCCHHHHHCCHHHHCCCCCEEECCCCCCCCCCCCCCCCEE
CRPAVAAELIQAAKAGGLPVSVKTRLGYTDVDEWREWLTHILKQDIANLSIHLRTRAEMS
ECHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEHHHHH
KVDAHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQC
CCCCCCHHCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHH
MNTKSTDEVRALLDDFERKYLDGIE
CCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377