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The map label for this gene is nagP

Identifier: 16077837

GI number: 16077837

Start: 840656

End: 842014

Strand: Direct

Name: nagP

Synonym: BSU07700

Alternate gene names: 16077837

Gene position: 840656-842014 (Clockwise)

Preceding gene: 16077832

Following gene: 16077839

Centisome position: 19.94

GC content: 47.24

Gene sequence:

>1359_bases
ATGCTTTCATTTTTACAAAAACTCGGGAAGTCATTTATGCTCCCGATTGCGGTTCTGCCTGCGGTTGGAATTATCCTTGC
GCTTGGCAGAGAGGATGTATTTAATATCCCGTTTGTCTATCAGGCGGGGACGGCGGTTTTTGATCATCTGCCGCTTATTT
TTGCGATCGGTATTGCCATCGGCATTTCAAAAGACAGCAATGGGGCAGCGGGTTTATCAGGGGCGATATCGTATCTGATG
CTTGACGCGGCAACAAAGACAATTGATAAAACAAATAACATGGCGGTGTTTGGGGGAATTATTGCCGGTTTAATTGCCGG
TTACACCTATAACCGCTTCAAGGATACCAAGCTGCCTGAATATCTCGGGTTCTTCAGTGGCAGACGGCTTGTGCCGATTT
TAACAGCCATTATTACGATCATTTTAGCGGGCATATTCGGAGTGGTCTGGCCGCCGATTCAATCGTGTATCAACTCGTTT
GGAGAGTGGATGCTCGGGCTCGGCGGCATCGGGGCAGGCATATTCGGCCTATTCAACAGGCTGTTAATTCCGCTTGGCCT
TCACCATGTGCTGAACAATATTTTCTGGTTCCAATTCGGAGAATATAACGGAGTGACAGGGGATTTGGCGCGCTTTTTCG
CGAAAGATCCGACTGCGGGCACATATATGACCGGATTTTTCCCAATCATGATGTTCGGTCTTCCGGCGGCGTGTCTGGCT
ATGGTTGTGACTGCGAAGCCGTCTAAACGGAAAGCGACGGCTGGAATGATGATTGGATTTGCTTTAACGGCGTTTATCAC
AGGGATTACGGAGCCGATTGAGTTTGCATTTATGTTTTTATCTCCGCTTTTATACGCTGTTCACGCGGTCTTAACCGGGT
TATCTTTATTTATCGTCAACTGGCTCGGCATCCGCTCAGGCTTCTCTTTTTCAGCCGGAGCTATCGATTATGTGCTCAGC
TATGGAATCGCGGAAAAACCGCTGCTTCTGCTTTTGGTAGGTATATGCTATGCGGCAGTATACTTTATCGTGTTTTATGT
ACTGATTAAGGCTTTGAATTTAAAAACGCCTGGACGGGAAGACGATGATGTGGATGAAGTGCTGGATGAAAATACTGTTC
AGGATGTAAATGAAAACATCATGCTGAAAGGGCTCGGCGGAAAAGAAAACCTTCAAACCATTGATCATTGCGCCACAAGG
CTGCGACTGACTGTGAAGGATACCGCTTTGGTGGATGAAGCACTGCTAAAGAAAGCAGGCGCAAAAGGGGTTGTCAAATC
GGGCGGGCAATCGGTTCAAGTCATTATTGGCCCGAATGTGGAATTTGCAGCTGAGGAATTGAGGGCTGCTGTCAAATAA

Upstream 100 bases:

>100_bases
ACGGACCTGGGAAAAAAGAAATACCCCCGGGAAAATTGGTATAGATCACTAGATATCTTATATGGTATATTTGAAAAAAA
AGGGTATGAGGGGGATGGGT

Downstream 100 bases:

>100_bases
AAAAGCGGAGAGGGCAACCTCTCCGCTTTTTCTTATTTATCTTCGTTATCCTTTGACGTTTCGGAAGAATCTTCGTCATG
TTTTGTGTAATCATAATCAG

Product: phosphotransferase system (PTS) N-acetylglucosamine-specific enzyme IICB component

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLSFLQKLGKSFMLPIAVLPAVGIILALGREDVFNIPFVYQAGTAVFDHLPLIFAIGIAIGISKDSNGAAGLSGAISYLM
LDAATKTIDKTNNMAVFGGIIAGLIAGYTYNRFKDTKLPEYLGFFSGRRLVPILTAIITIILAGIFGVVWPPIQSCINSF
GEWMLGLGGIGAGIFGLFNRLLIPLGLHHVLNNIFWFQFGEYNGVTGDLARFFAKDPTAGTYMTGFFPIMMFGLPAACLA
MVVTAKPSKRKATAGMMIGFALTAFITGITEPIEFAFMFLSPLLYAVHAVLTGLSLFIVNWLGIRSGFSFSAGAIDYVLS
YGIAEKPLLLLLVGICYAAVYFIVFYVLIKALNLKTPGREDDDVDEVLDENTVQDVNENIMLKGLGGKENLQTIDHCATR
LRLTVKDTALVDEALLKKAGAKGVVKSGGQSVQVIIGPNVEFAAEELRAAVK

Sequences:

>Translated_452_residues
MLSFLQKLGKSFMLPIAVLPAVGIILALGREDVFNIPFVYQAGTAVFDHLPLIFAIGIAIGISKDSNGAAGLSGAISYLM
LDAATKTIDKTNNMAVFGGIIAGLIAGYTYNRFKDTKLPEYLGFFSGRRLVPILTAIITIILAGIFGVVWPPIQSCINSF
GEWMLGLGGIGAGIFGLFNRLLIPLGLHHVLNNIFWFQFGEYNGVTGDLARFFAKDPTAGTYMTGFFPIMMFGLPAACLA
MVVTAKPSKRKATAGMMIGFALTAFITGITEPIEFAFMFLSPLLYAVHAVLTGLSLFIVNWLGIRSGFSFSAGAIDYVLS
YGIAEKPLLLLLVGICYAAVYFIVFYVLIKALNLKTPGREDDDVDEVLDENTVQDVNENIMLKGLGGKENLQTIDHCATR
LRLTVKDTALVDEALLKKAGAKGVVKSGGQSVQVIIGPNVEFAAEELRAAVK
>Mature_452_residues
MLSFLQKLGKSFMLPIAVLPAVGIILALGREDVFNIPFVYQAGTAVFDHLPLIFAIGIAIGISKDSNGAAGLSGAISYLM
LDAATKTIDKTNNMAVFGGIIAGLIAGYTYNRFKDTKLPEYLGFFSGRRLVPILTAIITIILAGIFGVVWPPIQSCINSF
GEWMLGLGGIGAGIFGLFNRLLIPLGLHHVLNNIFWFQFGEYNGVTGDLARFFAKDPTAGTYMTGFFPIMMFGLPAACLA
MVVTAKPSKRKATAGMMIGFALTAFITGITEPIEFAFMFLSPLLYAVHAVLTGLSLFIVNWLGIRSGFSFSAGAIDYVLS
YGIAEKPLLLLLVGICYAAVYFIVFYVLIKALNLKTPGREDDDVDEVLDENTVQDVNENIMLKGLGGKENLQTIDHCATR
LRLTVKDTALVDEALLKKAGAKGVVKSGGQSVQVIIGPNVEFAAEELRAAVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI1786894, Length=465, Percent_Identity=50.3225806451613, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787343, Length=473, Percent_Identity=43.5517970401691, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1787908, Length=515, Percent_Identity=32.621359223301, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTWCB_BACSU (O34521)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   D69810
- RefSeq:   NP_388651.1
- HSSP:   P69786
- ProteinModelPortal:   O34521
- SMR:   O34521
- EnsemblBacteria:   EBBACT00000002643
- GeneID:   938807
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07700
- NMPDR:   fig|224308.1.peg.770
- GenoList:   BSU07700
- GeneTree:   EBGT00050000000032
- HOGENOM:   HBG571563
- OMA:   TIDHCAT
- PhylomeDB:   O34521
- ProtClustDB:   CLSK886865
- BioCyc:   BSUB:BSU07700-MONOMER
- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR01998

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 48581; Mature: 48581

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 397-397

Signals:

None

Transmembrane regions:

HASH(0xd349a60)-; HASH(0xc798bf0)-; HASH(0xd1ab234)-; HASH(0xd213410)-; HASH(0xd6d989c)-; HASH(0xdd3c674)-; HASH(0xd4808dc)-; HASH(0xda8ab18)-; HASH(0xd6df924)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHCCCCEEEEEECCCCHHHHHHHHHHCH
>Mature Secondary Structure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CCCHHHCCCCEEEEEECCCCHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377