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The map label for this gene is nasF

Identifier: 16077397

GI number: 16077397

Start: 353900

End: 355351

Strand: Reverse

Name: nasF

Synonym: BSU03280

Alternate gene names: 16077397

Gene position: 355351-353900 (Counterclockwise)

Preceding gene: 16077398

Following gene: 16077395

Centisome position: 8.43

GC content: 49.38

Gene sequence:

>1452_bases
ATGATCATGAAGAACGGAATCGTATATTTCGTGGGAGCCGGACCCGGCGACCCAGGCCTGCTCACCATCAAAGGAAAACA
GGCGCTCAAGGAAGCAGATGTGATTTTATATGACCGGCTCGCCAACCCGAAGCTGCTCGAATTTGCATCACCGGACTGCC
AGTTTATTTATTGCGGAAAACTGCCAAACCGCCATTTTATGAAACAAAAAGAAATTAACGCCCTGCTGGTTGAAAAAGCA
TTAAATGGGTTAACCGTCGTTCGCTTGAAGGGCGGAGATCCGAGCGTATTCGGCAGAGTCGGCGAGGAAGCAGATGCTCT
TCATGAGCACGGCATTCGCTATGAAATGGTGCCGGGAATCACCTCTGGAATTGCAGCGCCGCTATATGCGGGCATCCCCG
TCACGCATCGGGATTTTGCATCGTCTTTTGCCATGATTACAGCGCATGATAAATCATTAAAAGGCACGCCGAACCTTGAC
TGGGAAGGGCTGGCCCGAAGCGTTCAAACCCTTGTTTTTTACATGGGTGTCAAGAACTTGTCTTATATTTGCCAGCAGCT
TATTTCATACGGGAAATCGCCTTCTGTCCCTGTCATTGTCATCCAATGGGGAACGTGGGGCCGCCAGCGGAGTGTGAAAG
GAACACTTGAGAACATTCAGCAAAAAGTGCAGGAACACCAGATTACAAACCCTGCGATTATCGTCATCGGAGACATCGTC
AACTTTCAAACACACAGCTGGTTTGAAAGCAAACCGCTCATCGGACGGCATTTGATGGTTGTGACTCATGGCGAAGATGA
AGACCCGCTTGCAGACAAGCTGCGCGATTCCGGCGCCGATCTCATCGAGTGGCCGAAATGGCGGACCGAAAACATGCCTG
TCAATGAAGAAATTCTTCGAAAGATTGGCACCTTTGAAGACGTTTTTTTCACATCGCGCCGGGCGGTTTGTGAATTTTTC
AGAGCACTGGCTTCTCAGAAAATCGACATCCGCCAGCTGACAGCCAAACTGAGTGCAGCATCTGAGCAAGCAAAGACAGA
GCTTGAGAAACGGGGCTTTCTTGTCACCGCCATTCAGCCTGATTCAGAGAAACGTCTTGTTGTCGGGAGCCGCCATGCAG
TGGAAAACATGCAAAAGCATGAATCCTGTTCGTTTTATATCACTCATGAAAACGTCATAGATGACCGGTTCACCCACATG
ATTCAGCGTACCATCAGTGAATCCCCGCTGCACATGGTCATTTGCCCAAACAAGCTGTCCGTTCAGCAATTGATCAATGG
TGGCGAACAAATTGGCATTTTGCCGGAACCGTCAGCAAGCCGCCCTCCTATCGTCTGCATAGGCGATGACTCAGCTGCCG
GCATTTATGGATTCACAGCCGTACAAGAACAGGATGAATTGCTAGCTTTCATCCACAATCAACATGCAGAGAAAAAACTC
CTTCACACCTGA

Upstream 100 bases:

>100_bases
AACGCTGATCGAAGGAGAACATGTTTATCTCGTATATTGATCAAAAGACCGGATCACTTTTCTAGTGGTCCTTCATTTTT
TACAGTGACGGAAGGATGAC

Downstream 100 bases:

>100_bases
TGGCAAGCAGCCCTTTTCCTCAAGGGCTGTTTTATTTATGCACCAATTTTTTATGATGGGTGGAATACATCGCCATATTC
TCGGCCTTAGGGTCCAGATA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MIMKNGIVYFVGAGPGDPGLLTIKGKQALKEADVILYDRLANPKLLEFASPDCQFIYCGKLPNRHFMKQKEINALLVEKA
LNGLTVVRLKGGDPSVFGRVGEEADALHEHGIRYEMVPGITSGIAAPLYAGIPVTHRDFASSFAMITAHDKSLKGTPNLD
WEGLARSVQTLVFYMGVKNLSYICQQLISYGKSPSVPVIVIQWGTWGRQRSVKGTLENIQQKVQEHQITNPAIIVIGDIV
NFQTHSWFESKPLIGRHLMVVTHGEDEDPLADKLRDSGADLIEWPKWRTENMPVNEEILRKIGTFEDVFFTSRRAVCEFF
RALASQKIDIRQLTAKLSAASEQAKTELEKRGFLVTAIQPDSEKRLVVGSRHAVENMQKHESCSFYITHENVIDDRFTHM
IQRTISESPLHMVICPNKLSVQQLINGGEQIGILPEPSASRPPIVCIGDDSAAGIYGFTAVQEQDELLAFIHNQHAEKKL
LHT

Sequences:

>Translated_483_residues
MIMKNGIVYFVGAGPGDPGLLTIKGKQALKEADVILYDRLANPKLLEFASPDCQFIYCGKLPNRHFMKQKEINALLVEKA
LNGLTVVRLKGGDPSVFGRVGEEADALHEHGIRYEMVPGITSGIAAPLYAGIPVTHRDFASSFAMITAHDKSLKGTPNLD
WEGLARSVQTLVFYMGVKNLSYICQQLISYGKSPSVPVIVIQWGTWGRQRSVKGTLENIQQKVQEHQITNPAIIVIGDIV
NFQTHSWFESKPLIGRHLMVVTHGEDEDPLADKLRDSGADLIEWPKWRTENMPVNEEILRKIGTFEDVFFTSRRAVCEFF
RALASQKIDIRQLTAKLSAASEQAKTELEKRGFLVTAIQPDSEKRLVVGSRHAVENMQKHESCSFYITHENVIDDRFTHM
IQRTISESPLHMVICPNKLSVQQLINGGEQIGILPEPSASRPPIVCIGDDSAAGIYGFTAVQEQDELLAFIHNQHAEKKL
LHT
>Mature_483_residues
MIMKNGIVYFVGAGPGDPGLLTIKGKQALKEADVILYDRLANPKLLEFASPDCQFIYCGKLPNRHFMKQKEINALLVEKA
LNGLTVVRLKGGDPSVFGRVGEEADALHEHGIRYEMVPGITSGIAAPLYAGIPVTHRDFASSFAMITAHDKSLKGTPNLD
WEGLARSVQTLVFYMGVKNLSYICQQLISYGKSPSVPVIVIQWGTWGRQRSVKGTLENIQQKVQEHQITNPAIIVIGDIV
NFQTHSWFESKPLIGRHLMVVTHGEDEDPLADKLRDSGADLIEWPKWRTENMPVNEEILRKIGTFEDVFFTSRRAVCEFF
RALASQKIDIRQLTAKLSAASEQAKTELEKRGFLVTAIQPDSEKRLVVGSRHAVENMQKHESCSFYITHENVIDDRFTHM
IQRTISESPLHMVICPNKLSVQQLINGGEQIGILPEPSASRPPIVCIGDDSAAGIYGFTAVQEQDELLAFIHNQHAEKKL
LHT

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=246, Percent_Identity=44.3089430894309, Blast_Score=211, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=33.6134453781513, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NASF_BACSU (P42437)

Other databases:

- EMBL:   D30689
- EMBL:   D50453
- EMBL:   AL009126
- PIR:   I40031
- RefSeq:   NP_388210.1
- ProteinModelPortal:   P42437
- SMR:   P42437
- EnsemblBacteria:   EBBACT00000003531
- GeneID:   938324
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03280
- NMPDR:   fig|224308.1.peg.329
- GenoList:   BSU03280
- GeneTree:   EBGT00050000002415
- HOGENOM:   HBG730212
- OMA:   PSMTIVG
- PhylomeDB:   P42437
- ProtClustDB:   CLSK886667
- BioCyc:   BSUB:BSU03280-MONOMER
- BRENDA:   2.1.1.107
- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- TIGRFAMs:   TIGR01469

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase; SSF53790 Cor/por_Metransf; SSF69618 HEM4_synth

EC number: =2.1.1.107

Molecular weight: Translated: 53870; Mature: 53870

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKNGIVYFVGAGPGDPGLLTIKGKQALKEADVILYDRLANPKLLEFASPDCQFIYCGK
CEEECCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCCCEEEECCCCCCEEEECC
LPNRHFMKQKEINALLVEKALNGLTVVRLKGGDPSVFGRVGEEADALHEHGIRYEMVPGI
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHCCCCHHHHHHHCCCEEEECCCC
TSGIAAPLYAGIPVTHRDFASSFAMITAHDKSLKGTPNLDWEGLARSVQTLVFYMGVKNL
HHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SYICQQLISYGKSPSVPVIVIQWGTWGRQRSVKGTLENIQQKVQEHQITNPAIIVIGDIV
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
NFQTHSWFESKPLIGRHLMVVTHGEDEDPLADKLRDSGADLIEWPKWRTENMPVNEEILR
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHH
KIGTFEDVFFTSRRAVCEFFRALASQKIDIRQLTAKLSAASEQAKTELEKRGFLVTAIQP
HHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
DSEKRLVVGSRHAVENMQKHESCSFYITHENVIDDRFTHMIQRTISESPLHMVICPNKLS
CCCCEEEEECHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VQQLINGGEQIGILPEPSASRPPIVCIGDDSAAGIYGFTAVQEQDELLAFIHNQHAEKKL
HHHHHCCCCEEECCCCCCCCCCCEEEECCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHH
LHT
CCC
>Mature Secondary Structure
MIMKNGIVYFVGAGPGDPGLLTIKGKQALKEADVILYDRLANPKLLEFASPDCQFIYCGK
CEEECCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCCCEEEECCCCCCEEEECC
LPNRHFMKQKEINALLVEKALNGLTVVRLKGGDPSVFGRVGEEADALHEHGIRYEMVPGI
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHCCCCHHHHHHHCCCEEEECCCC
TSGIAAPLYAGIPVTHRDFASSFAMITAHDKSLKGTPNLDWEGLARSVQTLVFYMGVKNL
HHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SYICQQLISYGKSPSVPVIVIQWGTWGRQRSVKGTLENIQQKVQEHQITNPAIIVIGDIV
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
NFQTHSWFESKPLIGRHLMVVTHGEDEDPLADKLRDSGADLIEWPKWRTENMPVNEEILR
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHH
KIGTFEDVFFTSRRAVCEFFRALASQKIDIRQLTAKLSAASEQAKTELEKRGFLVTAIQP
HHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
DSEKRLVVGSRHAVENMQKHESCSFYITHENVIDDRFTHMIQRTISESPLHMVICPNKLS
CCCCEEEEECHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VQQLINGGEQIGILPEPSASRPPIVCIGDDSAAGIYGFTAVQEQDELLAFIHNQHAEKKL
HHHHHCCCCEEECCCCCCCCCCCEEEECCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHH
LHT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377