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The map label for this gene is cwlK

Identifier: 16077350

GI number: 16077350

Start: 303804

End: 304307

Strand: Reverse

Name: cwlK

Synonym: BSU02810

Alternate gene names: 16077350

Gene position: 304307-303804 (Counterclockwise)

Preceding gene: 255767077

Following gene: 16077347

Centisome position: 7.22

GC content: 42.66

Gene sequence:

>504_bases
ATGAACTTACCCGCAAAAACATTTGTTATCTTATGTATTCTATTTCTTCTAGATCTTTGTTTTAGTTATATTCGGCATGA
ATGGCATTCTCAAAACGCCTTGCAGGACATGCCTGTCCCCTCGGATCTTCATCCGATTGTGAAGCAGAATGCCGATGCAC
TCAAAGCCGCTGCAGCGAATAAAGGCATTGACGTCGTCATTACTGAAGGATTCAGGTCCTTTAAGGAACAAGATGAATTA
TATAAACAGGGACGTACAAAAAAAGGGAATATTGTCACCTATGCAAGAGGAGGGGAATCGTATCATAACTATGGTTTAGC
GATTGATTTCGCCCTTCAAAAAAAGGATGGCAGCATCATATGGGATATGGAATATGACGGCAATCAAAACGGAAAATCAG
ATTGGCTGGAGGTTGTCGAGATCGCTAAGACACTCGGTTTCGAATGGGGCGGGGATTGGAAACGATTCAAGGACTACCCC
CACTTGGAGATGATTCCTAACTAG

Upstream 100 bases:

>100_bases
TACTATTTATGTTATATTTTATCATAGCTGTTCGCTTTTAGAGAGAGTCAATCACTTAAAAGCGTTAACATTCAGCACAT
CTTGAGAAAGTGAGACCGAT

Downstream 100 bases:

>100_bases
ACACCCCTCACAGCCTTTTCTTTTTCCTTATGTGCGCATCAATATAGGAAGGGTAAACCGGCTTATAGACAAGTTTGCAG
TGCTGGTCACTGCCTGCTGT

Product: murein L,D:-endopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDEL
YKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYP
HLEMIPN

Sequences:

>Translated_167_residues
MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDEL
YKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYP
HLEMIPN
>Mature_167_residues
MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDEL
YKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYP
HLEMIPN

Specific function: Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15C family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLK_BACSU (O34360)

Other databases:

- EMBL:   AB000617
- EMBL:   AL009126
- PIR:   F69755
- RefSeq:   NP_388163.1
- HSSP:   Q37979
- ProteinModelPortal:   O34360
- SMR:   O34360
- EnsemblBacteria:   EBBACT00000000351
- GeneID:   938378
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02810
- NMPDR:   fig|224308.1.peg.282
- GenoList:   BSU02810
- GeneTree:   EBGT00050000000337
- HOGENOM:   HBG314099
- OMA:   GGESYHN
- ProtClustDB:   CLSK886642
- BioCyc:   BSUB:BSU02810-MONOMER
- GO:   GO:0006508
- InterPro:   IPR009045
- InterPro:   IPR012340
- InterPro:   IPR003709
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF02557 VanY; SSF55166 Hedgehog_sig_N

EC number: NA

Molecular weight: Translated: 19113; Mature: 19113

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHCCHHHHHHHHCC
KGIDVVITEGFRSFKEQDELYKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSII
CCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCEE
WDMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYPHLEMIPN
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEECCC
>Mature Secondary Structure
MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHCCHHHHHHHHCC
KGIDVVITEGFRSFKEQDELYKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSII
CCCEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCEE
WDMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYPHLEMIPN
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377