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The map label for this gene is priA

Identifier: 15618833

GI number: 15618833

Start: 1055025

End: 1057274

Strand: Reverse

Name: priA

Synonym: CPn0924

Alternate gene names: 15618833

Gene position: 1057274-1055025 (Counterclockwise)

Preceding gene: 15618834

Following gene: 15618829

Centisome position: 85.94

GC content: 42.89

Gene sequence:

>2250_bases
ATGGGCTATATTGAATCGTCTACCTTTCGCCTATACGCCGAAGTCATCGTAGGCTCTAACATCAACAAGGTACTCGATTA
TGGCGTTCCTGAAAATCTAGAGCACATTACTAAAGGAACTGCTGTTACTATCTCTTTACGGGGAGGGAAAAAAGTTGGAG
TTATTTATCAAATAAAAACAACGACCCAATGTAAGAAAATTTTACCTATCTTAGGATTATCCGATTCAGAGATTGTCCTT
CCTCAAGATCTTCTAGACTTGCTATTTTGGATCAGCCAATACTACTTTGCTCCTCTTGGGAAAACTCTAAAATTATTTCT
TCCCGCTATCTCTTCGAATGTGATTCAGCCTAAGCAGCACTACCGTGTCGTCTTAAAGCAAAGTAAAGCAAAAACTAAAG
AGATTCTTGCAAAATTAGAAGTTTTACATCCTTCTCAAGGGGCTGTTTTAAAAATTCTACTACAACATGCGTCCCCACCG
GGTTTATCTTCCCTTATGGAGACTGCCAAAGTATCGCAATCTCCAATTCATTCTCTTGAAAAGCTGGGCATTCTTGATAT
TGTAGATGCAGCGCAGTTAGAGCTTCAAGAAGACCTCCTAACCTTTTTCCCGCCTGCTCCTAAGGATTTACATCCTGAAC
AGCAAAGTGCTATAGATAAAATTTTTTCCTCACTAAAAACCTCACAATTCCATACACATTTACTTTTCGGAATTACAGGA
AGTGGTAAAACAGAGATCTATCTTCGAGCAACAAGCGAGGCTCTTAAACAAGGGAAAAGTACAATTCTTCTTGTTCCAGA
GATCGCTCTCACAGTGCAGACAGTCTCATTATTTAAAGCGCGCTTTGGCAAGGATGTAGGTGTTCTCCATCACAAGCTTA
GCGACAGCGACAAAAGTCGCACGTGGCGCCAAGCTTCCGAAGGATCCCTACGTATCCTCATAGGACCACGGTCTGCTCTT
TTCTGCCCCATGAAGAATCTGGGACTGATCATTGTAGATGAAGAACACGATCCCGCCTATAAACAAACGGAAAGTCCTCC
TTGCTACCATGCCAGGGATGTCGCTGTAATGCGAGGCAAACTCGCTCATGCTACTGTGGTTTTAGGAAGTGCGACTCCCA
GCTTAGAAAGCTATACTAATGCGCTATCTGGCAAGTACGTTCTGTCTCGGCTCTCTTCAAGAGCAGCTGCTGCTCATCCC
GCAAAAATTTCTCTTATCAACATGAACTTAGAGAGGGAAAAGTCAAAAACCAAGATTCTATTTTCCCAGCCTGTGTTAAA
GAAAATAGCCGAACGTCTGGAAGTGGGAGAGCAGGTTTTGATTTTCTTTAATCGTCGGGGATATCATACCAACGTCTCTT
GTACTGTTTGCAAGCATACATTGAAATGCCCTCATTGTGACATGGTGCTCACCTTCCATAAATATGCAAATGTGCTTCTC
TGCCATCTATGCAACTCCTCACCTAAAGACCTCCCACAATCTTGTCCAAAATGTCTTGGAACTATGACGTTGCAATATCG
AGGCTCAGGAACAGAAAAAATAGAAAAAATTCTCCAGCAGATTTTTCCTCAGATACGTACCATCCGTATTGATTCAGACA
CTACCAAATTCAAGGGGAGCCATGAAACGTTACTCAGGCAATTTGCAACAGGAAAAGCAGATGTTTTGATCGGCACCCAG
ATGATTGCCAAAGGCATGAATTTCTCTGCAGTCACACTCGCGGTCATTCTAAATGGAGATTCTGGATTGTATATCCCTGA
TTTCCGAGCTTCAGAGCAAGTCTTCCAGCTCATTACACAGGTAGCAGGTAGGTCGGGTCGGAGCCACCTACCTGGAGAAA
TTCTCATCCAATCCTTTCTTCCTGACCATCCCACAATTCATAGTGCTATGCGTCAGGACTACTCGGCATTTTATAGTCAG
GAAATCACAGGTCGCGAACTCTGTGAGTATCCTCCATTTATCCGTCTTATCCGTTGTATTTTCATGGGGAAATGTCCCAA
GCAGACTTGGGAAGAGGCCCACCGAGTCCATAACATTCTAAAAGAGCAGCTAGAAAGCACGAATCCACTGATGCCAGTGA
CTCCTTGCGGTCATTTTAAAATCAAGGATACGTTCCGCTATCAGTTTTTAATTAAGAGTGCTTATGTCATTCCAGTGAAT
AAGAAACTCCATCACGCCTTAATGCTGGCAAAGCTCTCGCCAAAGGTGAAATTCATGATTGATGTGGACCCTATGACTAC
TTTTTTCTAA

Upstream 100 bases:

>100_bases
CAACTGATTATCATCAATCTCTGACACAAAATGGATGTATCTTTCTTCCCCTCTATTCTTCACTAGAGTATGAAAAAGAT
TCACAGCTAAAACGAAATCT

Downstream 100 bases:

>100_bases
GTATGGCTTCATAACTTGAGCCAACAGGTTCACTTCATCGACAGTATTGTAAGCGTGCAAGTTCACACGTAAGAAAGGAT
GCTTAGCAAAGGCAACAACG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MGYIESSTFRLYAEVIVGSNINKVLDYGVPENLEHITKGTAVTISLRGGKKVGVIYQIKTTTQCKKILPILGLSDSEIVL
PQDLLDLLFWISQYYFAPLGKTLKLFLPAISSNVIQPKQHYRVVLKQSKAKTKEILAKLEVLHPSQGAVLKILLQHASPP
GLSSLMETAKVSQSPIHSLEKLGILDIVDAAQLELQEDLLTFFPPAPKDLHPEQQSAIDKIFSSLKTSQFHTHLLFGITG
SGKTEIYLRATSEALKQGKSTILLVPEIALTVQTVSLFKARFGKDVGVLHHKLSDSDKSRTWRQASEGSLRILIGPRSAL
FCPMKNLGLIIVDEEHDPAYKQTESPPCYHARDVAVMRGKLAHATVVLGSATPSLESYTNALSGKYVLSRLSSRAAAAHP
AKISLINMNLEREKSKTKILFSQPVLKKIAERLEVGEQVLIFFNRRGYHTNVSCTVCKHTLKCPHCDMVLTFHKYANVLL
CHLCNSSPKDLPQSCPKCLGTMTLQYRGSGTEKIEKILQQIFPQIRTIRIDSDTTKFKGSHETLLRQFATGKADVLIGTQ
MIAKGMNFSAVTLAVILNGDSGLYIPDFRASEQVFQLITQVAGRSGRSHLPGEILIQSFLPDHPTIHSAMRQDYSAFYSQ
EITGRELCEYPPFIRLIRCIFMGKCPKQTWEEAHRVHNILKEQLESTNPLMPVTPCGHFKIKDTFRYQFLIKSAYVIPVN
KKLHHALMLAKLSPKVKFMIDVDPMTTFF

Sequences:

>Translated_749_residues
MGYIESSTFRLYAEVIVGSNINKVLDYGVPENLEHITKGTAVTISLRGGKKVGVIYQIKTTTQCKKILPILGLSDSEIVL
PQDLLDLLFWISQYYFAPLGKTLKLFLPAISSNVIQPKQHYRVVLKQSKAKTKEILAKLEVLHPSQGAVLKILLQHASPP
GLSSLMETAKVSQSPIHSLEKLGILDIVDAAQLELQEDLLTFFPPAPKDLHPEQQSAIDKIFSSLKTSQFHTHLLFGITG
SGKTEIYLRATSEALKQGKSTILLVPEIALTVQTVSLFKARFGKDVGVLHHKLSDSDKSRTWRQASEGSLRILIGPRSAL
FCPMKNLGLIIVDEEHDPAYKQTESPPCYHARDVAVMRGKLAHATVVLGSATPSLESYTNALSGKYVLSRLSSRAAAAHP
AKISLINMNLEREKSKTKILFSQPVLKKIAERLEVGEQVLIFFNRRGYHTNVSCTVCKHTLKCPHCDMVLTFHKYANVLL
CHLCNSSPKDLPQSCPKCLGTMTLQYRGSGTEKIEKILQQIFPQIRTIRIDSDTTKFKGSHETLLRQFATGKADVLIGTQ
MIAKGMNFSAVTLAVILNGDSGLYIPDFRASEQVFQLITQVAGRSGRSHLPGEILIQSFLPDHPTIHSAMRQDYSAFYSQ
EITGRELCEYPPFIRLIRCIFMGKCPKQTWEEAHRVHNILKEQLESTNPLMPVTPCGHFKIKDTFRYQFLIKSAYVIPVN
KKLHHALMLAKLSPKVKFMIDVDPMTTFF
>Mature_748_residues
GYIESSTFRLYAEVIVGSNINKVLDYGVPENLEHITKGTAVTISLRGGKKVGVIYQIKTTTQCKKILPILGLSDSEIVLP
QDLLDLLFWISQYYFAPLGKTLKLFLPAISSNVIQPKQHYRVVLKQSKAKTKEILAKLEVLHPSQGAVLKILLQHASPPG
LSSLMETAKVSQSPIHSLEKLGILDIVDAAQLELQEDLLTFFPPAPKDLHPEQQSAIDKIFSSLKTSQFHTHLLFGITGS
GKTEIYLRATSEALKQGKSTILLVPEIALTVQTVSLFKARFGKDVGVLHHKLSDSDKSRTWRQASEGSLRILIGPRSALF
CPMKNLGLIIVDEEHDPAYKQTESPPCYHARDVAVMRGKLAHATVVLGSATPSLESYTNALSGKYVLSRLSSRAAAAHPA
KISLINMNLEREKSKTKILFSQPVLKKIAERLEVGEQVLIFFNRRGYHTNVSCTVCKHTLKCPHCDMVLTFHKYANVLLC
HLCNSSPKDLPQSCPKCLGTMTLQYRGSGTEKIEKILQQIFPQIRTIRIDSDTTKFKGSHETLLRQFATGKADVLIGTQM
IAKGMNFSAVTLAVILNGDSGLYIPDFRASEQVFQLITQVAGRSGRSHLPGEILIQSFLPDHPTIHSAMRQDYSAFYSQE
ITGRELCEYPPFIRLIRCIFMGKCPKQTWEEAHRVHNILKEQLESTNPLMPVTPCGHFKIKDTFRYQFLIKSAYVIPVNK
KLHHALMLAKLSPKVKFMIDVDPMTTFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=750, Percent_Identity=32.9333333333333, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787357, Length=192, Percent_Identity=28.6458333333333, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_CHLPN (Q9Z6Y2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86606
- PIR:   C72018
- RefSeq:   NP_225119.1
- RefSeq:   NP_300981.1
- RefSeq:   NP_445479.1
- RefSeq:   NP_877228.1
- ProteinModelPortal:   Q9Z6Y2
- SMR:   Q9Z6Y2
- GeneID:   1467635
- GeneID:   895667
- GeneID:   919842
- GeneID:   963406
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0942
- KEGG:   cpn:CPn0924
- KEGG:   cpt:CpB0955
- TIGR:   CP_0942
- HOGENOM:   HBG725563
- OMA:   RTIRIDS
- PhylomeDB:   Q9Z6Y2
- ProtClustDB:   PRK05580
- BioCyc:   CPNE115711:CP_0942-MONOMER
- BioCyc:   CPNE115713:CPN0924-MONOMER
- BioCyc:   CPNE138677:CPJ0924-MONOMER
- BioCyc:   CPNE182082:CPB0955-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII

EC number: NA

Molecular weight: Translated: 83649; Mature: 83518

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYIESSTFRLYAEVIVGSNINKVLDYGVPENLEHITKGTAVTISLRGGKKVGVIYQIKT
CCCCCCCHHHEEEHHHHCCCHHHHHHCCCCCCHHHHCCCCEEEEEECCCCEEEEEEEEEC
TTQCKKILPILGLSDSEIVLPQDLLDLLFWISQYYFAPLGKTLKLFLPAISSNVIQPKQH
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHH
YRVVLKQSKAKTKEILAKLEVLHPSQGAVLKILLQHASPPGLSSLMETAKVSQSPIHSLE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHH
KLGILDIVDAAQLELQEDLLTFFPPAPKDLHPEQQSAIDKIFSSLKTSQFHTHLLFGITG
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEC
SGKTEIYLRATSEALKQGKSTILLVPEIALTVQTVSLFKARFGKDVGVLHHKLSDSDKSR
CCCEEEEEEECHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
TWRQASEGSLRILIGPRSALFCPMKNLGLIIVDEEHDPAYKQTESPPCYHARDVAVMRGK
HHHHCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
LAHATVVLGSATPSLESYTNALSGKYVLSRLSSRAAAAHPAKISLINMNLEREKSKTKIL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCEEE
FSQPVLKKIAERLEVGEQVLIFFNRRGYHTNVSCTVCKHTLKCPHCDMVLTFHKYANVLL
ECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHH
CHLCNSSPKDLPQSCPKCLGTMTLQYRGSGTEKIEKILQQIFPQIRTIRIDSDTTKFKGS
HHHCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
HETLLRQFATGKADVLIGTQMIAKGMNFSAVTLAVILNGDSGLYIPDFRASEQVFQLITQ
HHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEEEEEEECCCCEECCCCCCHHHHHHHHHH
VAGRSGRSHLPGEILIQSFLPDHPTIHSAMRQDYSAFYSQEITGRELCEYPPFIRLIRCI
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHH
FMGKCPKQTWEEAHRVHNILKEQLESTNPLMPVTPCGHFKIKDTFRYQFLIKSAYVIPVN
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHEEEEEEEEEEEEECC
KKLHHALMLAKLSPKVKFMIDVDPMTTFF
HHHHHHHHHHHCCCCEEEEEEECCHHCCC
>Mature Secondary Structure 
GYIESSTFRLYAEVIVGSNINKVLDYGVPENLEHITKGTAVTISLRGGKKVGVIYQIKT
CCCCCCHHHEEEHHHHCCCHHHHHHCCCCCCHHHHCCCCEEEEEECCCCEEEEEEEEEC
TTQCKKILPILGLSDSEIVLPQDLLDLLFWISQYYFAPLGKTLKLFLPAISSNVIQPKQH
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHH
YRVVLKQSKAKTKEILAKLEVLHPSQGAVLKILLQHASPPGLSSLMETAKVSQSPIHSLE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHH
KLGILDIVDAAQLELQEDLLTFFPPAPKDLHPEQQSAIDKIFSSLKTSQFHTHLLFGITG
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEC
SGKTEIYLRATSEALKQGKSTILLVPEIALTVQTVSLFKARFGKDVGVLHHKLSDSDKSR
CCCEEEEEEECHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
TWRQASEGSLRILIGPRSALFCPMKNLGLIIVDEEHDPAYKQTESPPCYHARDVAVMRGK
HHHHCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
LAHATVVLGSATPSLESYTNALSGKYVLSRLSSRAAAAHPAKISLINMNLEREKSKTKIL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCEEE
FSQPVLKKIAERLEVGEQVLIFFNRRGYHTNVSCTVCKHTLKCPHCDMVLTFHKYANVLL
ECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHH
CHLCNSSPKDLPQSCPKCLGTMTLQYRGSGTEKIEKILQQIFPQIRTIRIDSDTTKFKGS
HHHCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
HETLLRQFATGKADVLIGTQMIAKGMNFSAVTLAVILNGDSGLYIPDFRASEQVFQLITQ
HHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEEEEEEECCCCEECCCCCCHHHHHHHHHH
VAGRSGRSHLPGEILIQSFLPDHPTIHSAMRQDYSAFYSQEITGRELCEYPPFIRLIRCI
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHH
FMGKCPKQTWEEAHRVHNILKEQLESTNPLMPVTPCGHFKIKDTFRYQFLIKSAYVIPVN
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHEEEEEEEEEEEEECC
KKLHHALMLAKLSPKVKFMIDVDPMTTFF
HHHHHHHHHHHCCCCEEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362