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The map label for this gene is ppa

Identifier: 15618827

GI number: 15618827

Start: 1048576

End: 1049223

Strand: Reverse

Name: ppa

Synonym: CPn0918

Alternate gene names: 15618827

Gene position: 1049223-1048576 (Counterclockwise)

Preceding gene: 15618829

Following gene: 15618820

Centisome position: 85.29

GC content: 39.2

Gene sequence:

>648_bases
ATGTCTAAAAAACCATTATATGTTGCACATCCTTGGCACAGCCCTACATTAACCCAGGATAATTATGAGTCCCTGTGTTG
TTATATAGAAATTACTCCTTATGACTCTGTAAAATTTGAATTAGACAAGGCTACAGGCTTGCTCAAAGTAGACAGACCTC
AAAAATTTTCTAACTTTTGTCCCTGCCTTTATGGTTTACTGCCTCAAACATACTGTGGCACTGCTTCTGGGAACTATAGC
GGGGAACAAACACGTCGCGAAGGAATCCAAGGTGATAAAGACCCTCTGGATGTCTGTGTTCTTACAGAAAAAAATATCCA
TCATGGAAATATTTTACTTCAAGCACGTCCTATAGGAGGTCTTCGCATCATTGATTCTGGAGAAGCTGATGATAAGATTA
TTGCTGTTTTAGAGGACGATTTAGTCTTTGCTGAGATCGAAGATATTTCAGACTGTCCAGGCACAGTATTAGATATGATC
CAACACTATTTCCTAACATATAAAGCGACTCCCAATCATTTAATTAAAGGTAGCCCTGCAAAAATTGAAATCGTGGGGAT
TTACGGGAAGAAGGAAGCACAAAAGGTGATACAGCTCGCTCACGAGGATTACTTATCCTATATTGGAGACACTGCTGAAG
TGAATTAA

Upstream 100 bases:

>100_bases
TCAATTAGGCCTGAGCAGACACTTCTCTTAAAACTTTCTCCGTGTTAGTCTAAATTCACTAAACCCGATTGTATAGGAAA
TTTTATATTGAGAGTTACTT

Downstream 100 bases:

>100_bases
TGTCTAAGGAGAGGGGTTCCCTCTCCTTGAAAATCAAGAAGAGAAAAGATAGTTATTAATAAATTTATCTGCTTCTACGG
TAAGATCTCGTAGCTCAGGA

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSKKPLYVAHPWHSPTLTQDNYESLCCYIEITPYDSVKFELDKATGLLKVDRPQKFSNFCPCLYGLLPQTYCGTASGNYS
GEQTRREGIQGDKDPLDVCVLTEKNIHHGNILLQARPIGGLRIIDSGEADDKIIAVLEDDLVFAEIEDISDCPGTVLDMI
QHYFLTYKATPNHLIKGSPAKIEIVGIYGKKEAQKVIQLAHEDYLSYIGDTAEVN

Sequences:

>Translated_215_residues
MSKKPLYVAHPWHSPTLTQDNYESLCCYIEITPYDSVKFELDKATGLLKVDRPQKFSNFCPCLYGLLPQTYCGTASGNYS
GEQTRREGIQGDKDPLDVCVLTEKNIHHGNILLQARPIGGLRIIDSGEADDKIIAVLEDDLVFAEIEDISDCPGTVLDMI
QHYFLTYKATPNHLIKGSPAKIEIVGIYGKKEAQKVIQLAHEDYLSYIGDTAEVN
>Mature_214_residues
SKKPLYVAHPWHSPTLTQDNYESLCCYIEITPYDSVKFELDKATGLLKVDRPQKFSNFCPCLYGLLPQTYCGTASGNYSG
EQTRREGIQGDKDPLDVCVLTEKNIHHGNILLQARPIGGLRIIDSGEADDKIIAVLEDDLVFAEIEDISDCPGTVLDMIQ
HYFLTYKATPNHLIKGSPAKIEIVGIYGKKEAQKVIQLAHEDYLSYIGDTAEVN

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family

Homologues:

Organism=Escherichia coli, GI1790673, Length=168, Percent_Identity=31.547619047619, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): IPYR_CHLPN (Q9Z6Y8)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D86605
- PIR:   E72020
- RefSeq:   NP_225113.1
- RefSeq:   NP_300975.1
- RefSeq:   NP_445485.1
- RefSeq:   NP_877222.1
- ProteinModelPortal:   Q9Z6Y8
- SMR:   Q9Z6Y8
- PHCI-2DPAGE:   Q9Z6Y8
- GeneID:   1467629
- GeneID:   895310
- GeneID:   919683
- GeneID:   963413
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0948
- KEGG:   cpn:CPn0918
- KEGG:   cpt:CpB0950
- TIGR:   CP_0948
- HOGENOM:   HBG529150
- OMA:   IMVIMRE
- ProtClustDB:   PRK00642
- BioCyc:   CPNE115711:CP_0948-MONOMER
- BioCyc:   CPNE115713:CPN0918-MONOMER
- BioCyc:   CPNE138677:CPJ0918-MONOMER
- BioCyc:   CPNE182082:CPB0950-MONOMER
- BRENDA:   3.6.1.1
- GO:   GO:0005737
- HAMAP:   MF_00209
- InterPro:   IPR008162
- Gene3D:   G3DSA:3.90.80.10
- PANTHER:   PTHR10286

Pfam domain/function: PF00719 Pyrophosphatase; SSF50324 Pyrophosphatase

EC number: =3.6.1.1

Molecular weight: Translated: 23981; Mature: 23850

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKPLYVAHPWHSPTLTQDNYESLCCYIEITPYDSVKFELDKATGLLKVDRPQKFSNFC
CCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEECCCCCHHHHH
PCLYGLLPQTYCGTASGNYSGEQTRREGIQGDKDPLDVCVLTEKNIHHGNILLQARPIGG
HHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCC
LRIIDSGEADDKIIAVLEDDLVFAEIEDISDCPGTVLDMIQHYFLTYKATPNHLIKGSPA
EEEEECCCCCCEEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCCEECCCCC
KIEIVGIYGKKEAQKVIQLAHEDYLSYIGDTAEVN
EEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKKPLYVAHPWHSPTLTQDNYESLCCYIEITPYDSVKFELDKATGLLKVDRPQKFSNFC
CCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEECCCCCHHHHH
PCLYGLLPQTYCGTASGNYSGEQTRREGIQGDKDPLDVCVLTEKNIHHGNILLQARPIGG
HHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCC
LRIIDSGEADDKIIAVLEDDLVFAEIEDISDCPGTVLDMIQHYFLTYKATPNHLIKGSPA
EEEEECCCCCCEEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCCEECCCCC
KIEIVGIYGKKEAQKVIQLAHEDYLSYIGDTAEVN
EEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362