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The map label for this gene is lipA

Identifier: 15618741

GI number: 15618741

Start: 938824

End: 939747

Strand: Reverse

Name: lipA

Synonym: CPn0832

Alternate gene names: 15618741

Gene position: 939747-938824 (Counterclockwise)

Preceding gene: 15618742

Following gene: 15618737

Centisome position: 76.39

GC content: 45.67

Gene sequence:

>924_bases
ATGAAATGTAGACCAACTTTAAATACAGACCAACCTAGAGTGCGGAAAAAGCTTCCCGAGCGTTTCCCCAAGTGGTTACA
GAGGCCCTTACCACAAGGTTCTGCATTTCATGCTACGGACGCTACAATAAAACGCTCAGGAATGCCAACGGTATGCGAAG
AAGCTCTCTGCCCCAATCGAGCAGAATGTTGGTCTCGTAAAACTGCCACCTACCTTGCTCTCGGCGATGTCTGTACAAGA
AGTTGCGGTTTTTGCAATATTGGGCATTCTAAAACCCCACCCGCGCTAGATCCTACAGAACCCGAGCGCATTGCTTTGTC
AGCGAAAGAACTGGGGTTGAAGCATGTGGTAATCACCATGGTGGCTCGCGATGACCTTGAGGATGGTGGTGCACAAGGTT
TAGTCGATATCATTCAAAAGTTACGTGAAGAACTCCCTCAAGCGACTACAGAAGTTTTAGCTTCTGATTTCCAAGGGAAT
GTTTCTGCTCTCCACACCCTGTTAGATTCTGGCATTACTATTTATAATCATAATGTCGAAACTGTAGCGAGGCTCTCGCC
TCTAGTACGGCACAAAGCAACCTATGCCCGATCTATGTTCATGTTAGAACAAGCTGCCAACTACCTCCCCGATCTTAAAA
TCAAATCCGGGATCATGGTAGGATTAGGTGAGATGGAAGGAGAAGTCAAACAAACTCTCCAAGATCTGGCTTCCATAGGA
GTTCGTATTGTCACTATAGGTCAATACTTACGTCCTTCTCGTAAGCATCTCCAAGTCAAAAGTTATGTGACTCCCGAGAC
TTTTGATTACTATCGTCGAGTCGGAGAAGCTATGGGTCTTTTTGTTTACGCAGGACCTTTTGTACGCTCGAGTTTTAATG
CCGATATGATCCTAGCTTCTGTACAAGACAAAGCGTCAGCTTAA

Upstream 100 bases:

>100_bases
GCATATATGAAACCGTGCATGCTCATCCCACACTCTCTGAAGTTTGGGCTGAAGGTGCTTTACTTGCTACAAATCACCCT
TTACACTTCCCTCCTAAGTC

Downstream 100 bases:

>100_bases
CAAGCATAGTACGATCCATCTCATCGAATCTTGATATTGAAAGATCATGGATTCACATCTTAAAAACTACATTCTAACGC
TTTTTCAAACTGTCATCCTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKCRPTLNTDQPRVRKKLPERFPKWLQRPLPQGSAFHATDATIKRSGMPTVCEEALCPNRAECWSRKTATYLALGDVCTR
SCGFCNIGHSKTPPALDPTEPERIALSAKELGLKHVVITMVARDDLEDGGAQGLVDIIQKLREELPQATTEVLASDFQGN
VSALHTLLDSGITIYNHNVETVARLSPLVRHKATYARSMFMLEQAANYLPDLKIKSGIMVGLGEMEGEVKQTLQDLASIG
VRIVTIGQYLRPSRKHLQVKSYVTPETFDYYRRVGEAMGLFVYAGPFVRSSFNADMILASVQDKASA

Sequences:

>Translated_307_residues
MKCRPTLNTDQPRVRKKLPERFPKWLQRPLPQGSAFHATDATIKRSGMPTVCEEALCPNRAECWSRKTATYLALGDVCTR
SCGFCNIGHSKTPPALDPTEPERIALSAKELGLKHVVITMVARDDLEDGGAQGLVDIIQKLREELPQATTEVLASDFQGN
VSALHTLLDSGITIYNHNVETVARLSPLVRHKATYARSMFMLEQAANYLPDLKIKSGIMVGLGEMEGEVKQTLQDLASIG
VRIVTIGQYLRPSRKHLQVKSYVTPETFDYYRRVGEAMGLFVYAGPFVRSSFNADMILASVQDKASA
>Mature_307_residues
MKCRPTLNTDQPRVRKKLPERFPKWLQRPLPQGSAFHATDATIKRSGMPTVCEEALCPNRAECWSRKTATYLALGDVCTR
SCGFCNIGHSKTPPALDPTEPERIALSAKELGLKHVVITMVARDDLEDGGAQGLVDIIQKLREELPQATTEVLASDFQGN
VSALHTLLDSGITIYNHNVETVARLSPLVRHKATYARSMFMLEQAANYLPDLKIKSGIMVGLGEMEGEVKQTLQDLASIG
VRIVTIGQYLRPSRKHLQVKSYVTPETFDYYRRVGEAMGLFVYAGPFVRSSFNADMILASVQDKASA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=281, Percent_Identity=40.5693950177936, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=40, Blast_Score=201, Evalue=6e-52,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=44.2857142857143, Blast_Score=239, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI32564533, Length=284, Percent_Identity=41.9014084507042, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=43.9716312056738, Blast_Score=268, Evalue=7e-73,
Organism=Drosophila melanogaster, GI221513272, Length=281, Percent_Identity=40.9252669039146, Blast_Score=243, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CHLPN (Q9Z774)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AB035942
- EMBL:   AE009440
- PIR:   B72031
- PIR:   F86594
- RefSeq:   NP_225027.1
- RefSeq:   NP_300889.1
- RefSeq:   NP_445575.1
- RefSeq:   NP_877133.1
- ProteinModelPortal:   Q9Z774
- GeneID:   1467540
- GeneID:   894731
- GeneID:   919598
- GeneID:   963516
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP1038
- KEGG:   cpn:CPn0832
- KEGG:   cpt:CpB0861
- TIGR:   CP_1038
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q9Z774
- ProtClustDB:   PRK05481
- BioCyc:   CPNE115711:CP_1038-MONOMER
- BioCyc:   CPNE115713:CPN0832-MONOMER
- BioCyc:   CPNE138677:CPJ0832-MONOMER
- BioCyc:   CPNE182082:CPB0861-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33994; Mature: 33994

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCRPTLNTDQPRVRKKLPERFPKWLQRPLPQGSAFHATDATIKRSGMPTVCEEALCPNR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHCCCCCHHHHHHCCCCH
AECWSRKTATYLALGDVCTRSCGFCNIGHSKTPPALDPTEPERIALSAKELGLKHVVITM
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEHHHHHHCHHHHHHHH
VARDDLEDGGAQGLVDIIQKLREELPQATTEVLASDFQGNVSALHTLLDSGITIYNHNVE
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHH
TVARLSPLVRHKATYARSMFMLEQAANYLPDLKIKSGIMVGLGEMEGEVKQTLQDLASIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECHHHHHHHHHHHHHHHHCC
VRIVTIGQYLRPSRKHLQVKSYVTPETFDYYRRVGEAMGLFVYAGPFVRSSFNADMILAS
EEEEEHHHHHCCHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHEECHHHHCCCCCHHEEEE
VQDKASA
HHHHCCC
>Mature Secondary Structure
MKCRPTLNTDQPRVRKKLPERFPKWLQRPLPQGSAFHATDATIKRSGMPTVCEEALCPNR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHCCCCCHHHHHHCCCCH
AECWSRKTATYLALGDVCTRSCGFCNIGHSKTPPALDPTEPERIALSAKELGLKHVVITM
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEHHHHHHCHHHHHHHH
VARDDLEDGGAQGLVDIIQKLREELPQATTEVLASDFQGNVSALHTLLDSGITIYNHNVE
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHH
TVARLSPLVRHKATYARSMFMLEQAANYLPDLKIKSGIMVGLGEMEGEVKQTLQDLASIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECHHHHHHHHHHHHHHHHCC
VRIVTIGQYLRPSRKHLQVKSYVTPETFDYYRRVGEAMGLFVYAGPFVRSSFNADMILAS
EEEEEHHHHHCCHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHEECHHHHCCCCCHHEEEE
VQDKASA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362