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The map label for this gene is gapA

Identifier: 15618534

GI number: 15618534

Start: 717008

End: 718015

Strand: Reverse

Name: gapA

Synonym: CPn0624

Alternate gene names: 15618534

Gene position: 718015-717008 (Counterclockwise)

Preceding gene: 15618535

Following gene: 15618533

Centisome position: 58.36

GC content: 38.69

Gene sequence:

>1008_bases
ATGAAAGTTGTAATTAATGGTTTCGGGCGAATTGGTCGTTTAGTTTTAAGACAAATCTTAAAGAGAAACTCTTCAGTAGA
AGTCCTCGCCATTAATGATCTTGTTCCTGGGGATGCGCTTACCTATCTATTTAAATTCGACTCTACACATGGACGTTTTC
CTGAGGATGTACGTTGTGAAGCGGACCACCTCATCGTTGGGAAGAGAAAAATTCAATTTTTATCTGAACGCAATGTTCAA
AATCTTCCTTGGAAAGATTTAGGGGTTGATCTCGTTATTGAATGTACAGGATTGTTCACAAAAAAAGAAGATGCTGAGAA
GCATATTCAAGCTGGAGCGAAACGAGTCTTAATCTCTGCTCCTGGAAAAGGTGATATTCCTACTTTCGTTATGGGAGTGA
ACCATAAGACTTTCAATCCAGAAAAAGACTTTGTTATATCGAATGCTTCTTGCACTACGAATTGTTTAGCTCCTATTGCT
AAAGTTTTACTAGATAATTTCGGAATTACAGAAGGTTTGATGACAACAGTTCATGCTGCTACTGCTACTCAACTAGTTGT
TGACGGACCTTCTAAGAAAGATTGGAGAGGAGGTCGTGGATGTTTACAAAATATTATTCCCGCCTCAACCGGAGCTGCAA
AAGCTGTAACTCTATGTCTTCCCGAGTTAAAGGGAAAATTAACAGGAATGGCTTTTCGGGTTCCTATCGAAGACGTGTCT
GTAGTTGACTTGACCGTCAGGTTAGATAAGTCTACGACATACGATGACATTTGCAAAGCTATGAAACAGGCTTCAGAAAC
TGATTTAAAAGGCATTTTAGATTATACAGATGAGCAGGTAGTTTCTTCAGATTTTATAGGATCTGAGTACTCCTCGATAT
TCGATGCTCTAGCTGGTATCGCTTTGAATGATCGGTTCTTCAAGTTAGTTGCGTGGTATGATAACGAAACAGGATATGCC
ACGCGTATAGTAGACTTATTAGAGTATGTAGAAAAAAACTCTAAATAA

Upstream 100 bases:

>100_bases
TGCAAAATAGAATTGGTGATAATGCTCAAAAGTGTATTATAGAGTTTTTAGCTAGCTAAAGCTAATCAATTTCCAATTTA
TTGAACTCTGAGGGTAAGCG

Downstream 100 bases:

>100_bases
AAGGTTATAATTACGTGTATTTTACAAGAGACCCCGTCATAGAAACTGTAATTACCTCTCGAGAGGGGTATAAGTTGTCG
GTAAGGAATACAAAGCATTT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQ
NLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIA
KVLLDNFGITEGLMTTVHAATATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVS
VVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGIALNDRFFKLVAWYDNETGYA
TRIVDLLEYVEKNSK

Sequences:

>Translated_335_residues
MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQ
NLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIA
KVLLDNFGITEGLMTTVHAATATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVS
VVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGIALNDRFFKLVAWYDNETGYA
TRIVDLLEYVEKNSK
>Mature_335_residues
MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQ
NLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIA
KVLLDNFGITEGLMTTVHAATATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVS
VVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGIALNDRFFKLVAWYDNETGYA
TRIVDLLEYVEKNSK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=331, Percent_Identity=59.5166163141994, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=56.4954682779456, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=59.9397590361446, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789295, Length=328, Percent_Identity=40.5487804878049, Blast_Score=261, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=58.9820359281437, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17568413, Length=334, Percent_Identity=58.9820359281437, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17534677, Length=334, Percent_Identity=58.6826347305389, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17534679, Length=334, Percent_Identity=58.6826347305389, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322468, Length=333, Percent_Identity=60.0600600600601, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321631, Length=333, Percent_Identity=60.0600600600601, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=58.5585585585586, Blast_Score=399, Evalue=1e-112,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=58.3081570996979, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=58.3081570996979, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17933600, Length=331, Percent_Identity=57.4018126888217, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI18110149, Length=331, Percent_Identity=57.4018126888217, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI19922412, Length=329, Percent_Identity=57.1428571428571, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_CHLPN (Q9Z7T0)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72053
- PIR:   E86568
- RefSeq:   NP_224820.1
- RefSeq:   NP_300680.1
- RefSeq:   NP_444675.1
- RefSeq:   NP_876922.1
- ProteinModelPortal:   Q9Z7T0
- SMR:   Q9Z7T0
- PHCI-2DPAGE:   Q9Z7T0
- GeneID:   1467329
- GeneID:   895404
- GeneID:   919389
- GeneID:   963037
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0123
- KEGG:   cpn:CPn0624
- KEGG:   cpt:CpB0650
- TIGR:   CP_0123
- HOGENOM:   HBG571736
- OMA:   DLKWDAI
- PhylomeDB:   Q9Z7T0
- ProtClustDB:   CLSK871427
- BioCyc:   CPNE115711:CP_0123-MONOMER
- BioCyc:   CPNE115713:CPN0624-MONOMER
- BioCyc:   CPNE138677:CPJ0624-MONOMER
- BioCyc:   CPNE182082:CPB0650-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 36838; Mature: 36838

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 151-151 BINDING 33-33 BINDING 77-77 BINDING 181-181 BINDING 233-233 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCE
CEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
ADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISA
CCEEEECHHHHHHHHHCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEC
PGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIAKVLLDNFGITEGLMTTVHAA
CCCCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVS
HHEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCE
VVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGI
EEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCC
ALNDRFFKLVAWYDNETGYATRIVDLLEYVEKNSK
EECCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCE
CEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCC
ADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISA
CCEEEECHHHHHHHHHCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEC
PGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIAKVLLDNFGITEGLMTTVHAA
CCCCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVS
HHEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCE
VVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGI
EEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCC
ALNDRFFKLVAWYDNETGYATRIVDLLEYVEKNSK
EECCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362