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The map label for this gene is tyrS

Identifier: 15618276

GI number: 15618276

Start: 405379

End: 406647

Strand: Reverse

Name: tyrS

Synonym: CPn0361

Alternate gene names: 15618276

Gene position: 406647-405379 (Counterclockwise)

Preceding gene: 15618277

Following gene: 15618275

Centisome position: 33.05

GC content: 41.53

Gene sequence:

>1269_bases
TTGGCAATTCTTAATTATGTACGATCACTCATGCAATCCTGGTTACAATCTTTACAAGAGCGAAATATTTTAGAGAATTT
TACCGCAGGTTTGGAATCCGTAGAGGGACCTATCGCCGCTTATTTAGGATTTGATCCTACCGCACCTGCTCTACATATTG
GTCATTGGATTGGGATTTGTTTCTTGAAGAGACTCGCTGCTCTGGGGATTACCCCCATAGCTTTAGTCGGGGGAGCCACA
GGTATGGTTGGAGATCCCTCAGGGAAACAGAGCGAGAGATCGTTACTTCAGACAAGTGAAGTTTTTGATAACAGTCAAAA
GATCACGGCGTGTCTCCAGCGCTATCTTCCCGGGGTGACTCTTGTAAATAATGCAGACTGGTTGCAGGAGATCTCCCTGA
TTGATTTCTTAGGGGATATAGGAAAACACTTTCGTTTAGGCCAAATGCTAGTGAAAGATACAATAAAGCAGCGGGTGCAT
TCTGATGAAGGAATTAGCTATACCGAGTTTAGCTATTTAATCCTGCAATCCTATGATTTTTATCACTTATTTAAAAATTA
TGGCACGATCTTGCAGTGCGGTGGTAGCGATCAGTGGGGGAATATTACTTCAGGAATCGATTTTATTCGCCGTAAAGGGT
TGGGTCAGGCCTACGGCCTTACCTATCCTTTATTAACGAATGCTCAGGGGAAAAAAATAGGGAAAACAGAGTCGGGAACT
GTATGGCTCGATTCAGATTTAACCTCTCCTTTTGAGCTGTACCAATACTTACTCCGTTTGCCCGATGATACCATCCCTAA
AATTGCTCGTACGTTAACTTTATTGAGCAATGAAGAAATTCAAGATATTGATAGGCGTGTACAGACGGATCCAGTTGCAG
TGAAGGAATTTGTAGCCCAAGATATCTTAAGTGCTATTCATGGAGATCTAGGGCTTGAAGAGGCTCTTTCTGTAACTCGT
AGCATGCATCCAGGGAATCTTTCATCCTTATCGGAAAAAGATTTTCATGAATTGTTTGCAGGAGGGATGGGGGCCTCATT
GGATAAATCCGAGGTGTTAGGGAAACGTTGGTTAGACCTATTTCTTGTTTTGGGACTATGTAAATCTAAAGGGGAAATTC
GAAGGCTAATTGAACAAAAAGGGGTATATATTAATAATGTGCCCATCGCTAATGAGCATAGTGTTTGTGAAGAACAAGAC
ATCTGTTATGGTCACTATGTGTTGTTGGCTCAAGGTAAAAAACGAAAGCTTGTTCTATATTTAAATTAG

Upstream 100 bases:

>100_bases
TGGTATCTCAGCAAAGGAATTATTCTCAGCGTACAATCTTTTCTTTATTGTTAGATAGGTGGTGCCTTGCATATAAGCTT
TAGGATCGATAAGATAGAGC

Downstream 100 bases:

>100_bases
TTTAAGGAGGCTAGGTAGCTTTGCAAACGAATATTGGTCTTATTGGCTTAGCTGTCATGGGGAAAAATCTTGTCTTAAAC
ATGATAGATCATGGTTTTTC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGAT
GMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLGDIGKHFRLGQMLVKDTIKQRVH
SDEGISYTEFSYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTR
SMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQD
ICYGHYVLLAQGKKRKLVLYLN

Sequences:

>Translated_422_residues
MAILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGAT
GMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLGDIGKHFRLGQMLVKDTIKQRVH
SDEGISYTEFSYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTR
SMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQD
ICYGHYVLLAQGKKRKLVLYLN
>Mature_421_residues
AILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGATG
MVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLGDIGKHFRLGQMLVKDTIKQRVHS
DEGISYTEFSYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTV
WLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTRS
MHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDI
CYGHYVLLAQGKKRKLVLYLN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=384, Percent_Identity=36.4583333333333, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1787925, Length=414, Percent_Identity=40.3381642512077, Blast_Score=294, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI25145802, Length=319, Percent_Identity=40.1253918495298, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25145805, Length=252, Percent_Identity=37.6984126984127, Blast_Score=151, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6325160, Length=381, Percent_Identity=32.0209973753281, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI20130383, Length=386, Percent_Identity=35.4922279792746, Blast_Score=254, Evalue=6e-68,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46978; Mature: 46846

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGIC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
FLKRLAALGITPIALVGGATGMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVT
HHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
LVNNADWLQEISLIDFLGDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEFSYLILQSYDF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
YHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQ
EEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
DILSAIHGDLGLEEALSVTRSMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDL
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLAQGKKRKLVLY
HHHHHHHHCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEE
LN
EC
>Mature Secondary Structure 
AILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
FLKRLAALGITPIALVGGATGMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVT
HHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
LVNNADWLQEISLIDFLGDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEFSYLILQSYDF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
YHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQ
EEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
DILSAIHGDLGLEEALSVTRSMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDL
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLAQGKKRKLVLY
HHHHHHHHCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEE
LN
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]