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The map label for this gene is yaeM

Identifier: 15618260

GI number: 15618260

Start: 387433

End: 388572

Strand: Reverse

Name: yaeM

Synonym: CPn0345

Alternate gene names: 15618260

Gene position: 388572-387433 (Counterclockwise)

Preceding gene: 15618261

Following gene: 15618259

Centisome position: 31.59

GC content: 40.35

Gene sequence:

>1140_bases
TTGAAACATTTAGCCGTTCTTGGGTCAACAGGTAGTATTGGCCGTCAAACATTAGAGATTGTGCGGCGCTATCCTTCAGA
ATTTAAAATTATTTCTATGGCTTCTTATGGAAATAATCTAAGGTTATTTTTTCAGCAACTAGAGGAGTTTGCTCCGTTAG
CCGCAGCGGTCTATAACGAAGAGGTTTATAACGAGGCCTGTCAGCGATTCCCCCATATGCAATTTTTCCTAGGCCAGGAG
GGTTTAACCCAACTTTGTATCATGGATACAGTCACTACTGTCGTTGCTGCTTCTTCAGGAATCGAGGCGCTACCCGCGAT
TCTAGAGTCGATGAAAAAAGGAAAAGCACTAGCTTTAGCAAACAAAGAAATTTTAGTTTGTGCTGGCGAATTGGTTTCTA
AGACTGCAAAGGAAAATGGTATAAAAGTTCTTCCTATTGATAGCGAGCATAATGCTTTGTATCAATGTTTAGAAGGCAGG
ACGATTGAGGGAATCAAGAAACTGATTCTTACAGCTTCTGGAGGGCCTCTGCTCAACAAGTCTTTAGAAGAGCTTTCTTG
TGTAACAAAACAAGATGTTTTGAACCATCCTATATGGAATATGGGTTCAAAAGTGACTGTGGACTCATCCACATTGGTCA
ATAAGGGACTCGAAATTATCGAGGCGTATTGGCTGTTTGGTTTAGAAAATGTTGAAATCCTGGCTGTAATTCATCCTCAG
AGCTTAATCCATGGTATGGTAGAGTTTTTAGATGGGAGTGTGATTTCTATCATGAATCCGCCTGATATGCTCTTCCCAAT
ACAATACGCTTTAACAGCTCCAGAGCGTTTTGCATCTCCTAGGGATGGTATGGATTTTTCGAAGAAACAAACTTTAGAAT
TTTTTCCGGTAGATGAGGAGCGATTTCCTAGTATCCGTTTAGCACAACAGGTATTAGAGAAACAGGGGTCTTCTGGAAGC
TTTTTTAATGCAGCCAATGAAGTATTAGTGCGGAGGTTCCTTTGCGAAGAGATTTCTTGGTGTGACATTTTACGCAAATT
AACGACTCTTATGGAATGTCATAAGGTTTATGCCTGCCACTCTTTAGAAGATATTTTAGAAGTAGATGGTGAGGCTAGAG
CTCTTGCTCAAGAAATATAA

Upstream 100 bases:

>100_bases
GTTTAGTCCACTTTAGTTAGAAATAGTATCAATAGAGAATTGACAATTCCTCGACTTGCGGAGTATGATTCTCTTTTTCA
CCTAGTTAAAGGTAGCATGC

Downstream 100 bases:

>100_bases
TCGAGTAGGTATATGACAATAATCTATTTTATTCTAGCAGCCCTAGCTTTAGGGATTTTAGTGTTAATTCATGAACTTGG
TCATCTGGTAGTAGCAAAAG

Product: 1-deoxy-D-xylulose 5-phosphate reductoisomerase

Products: NA

Alternate protein names: DXP reductoisomerase; 1-deoxyxylulose-5-phosphate reductoisomerase; 2-C-methyl-D-erythritol 4-phosphate synthase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKHLAVLGSTGSIGRQTLEIVRRYPSEFKIISMASYGNNLRLFFQQLEEFAPLAAAVYNEEVYNEACQRFPHMQFFLGQE
GLTQLCIMDTVTTVVAASSGIEALPAILESMKKGKALALANKEILVCAGELVSKTAKENGIKVLPIDSEHNALYQCLEGR
TIEGIKKLILTASGGPLLNKSLEELSCVTKQDVLNHPIWNMGSKVTVDSSTLVNKGLEIIEAYWLFGLENVEILAVIHPQ
SLIHGMVEFLDGSVISIMNPPDMLFPIQYALTAPERFASPRDGMDFSKKQTLEFFPVDEERFPSIRLAQQVLEKQGSSGS
FFNAANEVLVRRFLCEEISWCDILRKLTTLMECHKVYACHSLEDILEVDGEARALAQEI

Sequences:

>Translated_379_residues
MKHLAVLGSTGSIGRQTLEIVRRYPSEFKIISMASYGNNLRLFFQQLEEFAPLAAAVYNEEVYNEACQRFPHMQFFLGQE
GLTQLCIMDTVTTVVAASSGIEALPAILESMKKGKALALANKEILVCAGELVSKTAKENGIKVLPIDSEHNALYQCLEGR
TIEGIKKLILTASGGPLLNKSLEELSCVTKQDVLNHPIWNMGSKVTVDSSTLVNKGLEIIEAYWLFGLENVEILAVIHPQ
SLIHGMVEFLDGSVISIMNPPDMLFPIQYALTAPERFASPRDGMDFSKKQTLEFFPVDEERFPSIRLAQQVLEKQGSSGS
FFNAANEVLVRRFLCEEISWCDILRKLTTLMECHKVYACHSLEDILEVDGEARALAQEI
>Mature_379_residues
MKHLAVLGSTGSIGRQTLEIVRRYPSEFKIISMASYGNNLRLFFQQLEEFAPLAAAVYNEEVYNEACQRFPHMQFFLGQE
GLTQLCIMDTVTTVVAASSGIEALPAILESMKKGKALALANKEILVCAGELVSKTAKENGIKVLPIDSEHNALYQCLEGR
TIEGIKKLILTASGGPLLNKSLEELSCVTKQDVLNHPIWNMGSKVTVDSSTLVNKGLEIIEAYWLFGLENVEILAVIHPQ
SLIHGMVEFLDGSVISIMNPPDMLFPIQYALTAPERFASPRDGMDFSKKQTLEFFPVDEERFPSIRLAQQVLEKQGSSGS
FFNAANEVLVRRFLCEEISWCDILRKLTTLMECHKVYACHSLEDILEVDGEARALAQEI

Specific function: Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP)

COG id: COG0743

COG function: function code I; 1-deoxy-D-xylulose 5-phosphate reductoisomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DXR family

Homologues:

Organism=Escherichia coli, GI1786369, Length=393, Percent_Identity=35.6234096692112, Blast_Score=245, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DXR_CHLPN (Q9Z8J8)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72091
- PIR:   C81578
- PIR:   G86533
- RefSeq:   NP_224545.1
- RefSeq:   NP_300402.1
- RefSeq:   NP_444964.1
- RefSeq:   NP_876626.1
- ProteinModelPortal:   Q9Z8J8
- SMR:   Q9Z8J8
- GeneID:   1467033
- GeneID:   895172
- GeneID:   919117
- GeneID:   963687
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0415
- KEGG:   cpn:CPn0345
- KEGG:   cpt:CpB0352
- TIGR:   CP_0415
- HOGENOM:   HBG430762
- OMA:   IHSMVEY
- PhylomeDB:   Q9Z8J8
- ProtClustDB:   PRK05447
- BioCyc:   CPNE115711:CP_0415-MONOMER
- BioCyc:   CPNE115713:CPN0345-MONOMER
- BioCyc:   CPNE138677:CPJ0344-MONOMER
- BioCyc:   CPNE182082:CPB0352-MONOMER
- BRENDA:   1.1.1.267
- HAMAP:   MF_00183
- InterPro:   IPR003821
- InterPro:   IPR013644
- InterPro:   IPR013512
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF006205
- TIGRFAMs:   TIGR00243

Pfam domain/function: PF08436 DXP_redisom_C; PF02670 DXP_reductoisom

EC number: =1.1.1.267

Molecular weight: Translated: 42150; Mature: 42150

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: BINDING 122-122 BINDING 149-149 BINDING 173-173 BINDING 196-196 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLAVLGSTGSIGRQTLEIVRRYPSEFKIISMASYGNNLRLFFQQLEEFAPLAAAVYNE
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
EVYNEACQRFPHMQFFLGQEGLTQLCIMDTVTTVVAASSGIEALPAILESMKKGKALALA
HHHHHHHHHCCCCEEHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
NKEILVCAGELVSKTAKENGIKVLPIDSEHNALYQCLEGRTIEGIKKLILTASGGPLLNK
CCCEEEECHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
SLEELSCVTKQDVLNHPIWNMGSKVTVDSSTLVNKGLEIIEAYWLFGLENVEILAVIHPQ
HHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHH
SLIHGMVEFLDGSVISIMNPPDMLFPIQYALTAPERFASPRDGMDFSKKQTLEFFPVDEE
HHHHHHHHHHCCCEEEECCCCCCEEHHHHHHCCHHHHCCCCCCCCCCCCCCEEEECCCHH
RFPSIRLAQQVLEKQGSSGSFFNAANEVLVRRFLCEEISWCDILRKLTTLMECHKVYACH
HCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLEDILEVDGEARALAQEI
HHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MKHLAVLGSTGSIGRQTLEIVRRYPSEFKIISMASYGNNLRLFFQQLEEFAPLAAAVYNE
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
EVYNEACQRFPHMQFFLGQEGLTQLCIMDTVTTVVAASSGIEALPAILESMKKGKALALA
HHHHHHHHHCCCCEEHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
NKEILVCAGELVSKTAKENGIKVLPIDSEHNALYQCLEGRTIEGIKKLILTASGGPLLNK
CCCEEEECHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
SLEELSCVTKQDVLNHPIWNMGSKVTVDSSTLVNKGLEIIEAYWLFGLENVEILAVIHPQ
HHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECHH
SLIHGMVEFLDGSVISIMNPPDMLFPIQYALTAPERFASPRDGMDFSKKQTLEFFPVDEE
HHHHHHHHHHCCCEEEECCCCCCEEHHHHHHCCHHHHCCCCCCCCCCCCCCEEEECCCHH
RFPSIRLAQQVLEKQGSSGSFFNAANEVLVRRFLCEEISWCDILRKLTTLMECHKVYACH
HCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLEDILEVDGEARALAQEI
HHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362