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The map label for this gene is pfkA_1

Identifier: 15618084

GI number: 15618084

Start: 202124

End: 203791

Strand: Reverse

Name: pfkA_1

Synonym: CPn0160

Alternate gene names: 15618084

Gene position: 203791-202124 (Counterclockwise)

Preceding gene: 15618085

Following gene: 15618083

Centisome position: 16.57

GC content: 39.69

Gene sequence:

>1668_bases
GTGGAACTTCTCTCGTTAAATAAAAGTTATTTTGAAATCCAACGCCTTCGCTACCGCCCTGAGATTCTTACTTTATTAGA
AACGATACGTTCGAAACATATCCAAGAAACTTCGTCTCCCCCTTCTCCCCCTCCAGAGTTGCAAAAACACATTCCCAATC
TATGTCGGATCCCTGAAGTTTCTATTTATACTGAACAGGAAACGTCATCAAAACCATTAAAGATTGGGGTTTTACTATCA
GGAGGACAAGCTCCTGGGGGGCATAATGTCGTTATTGGTCTTTTTGATGCTTTACGAGTATTCAATCCCAAGACTCGCTT
ATTTGGATTCATCAAAGGACCTTTAGGGCTTACTCGTGGGCTCTATAAAGATCTGGATATCTCCGTAATCTATGATTATT
ACAACATGGGAGGGTTCGACATGCTCTCCTCAAGCCGAGAAAAAATTAAAACTGAAGAGCAGAAAAAAAACATCCTCAAC
ACAGTCAAGCAACTAAAGTTAGACGGACTACTCATTATAGGAGGAAATAATTCCAATACGGACACTGCGATGCTTGCAGA
ATATTTCCTTGCTCACAACTGCAAAACATCCGTGATTGGAGTCCCTAAAACTATAGATGGTGATCTTAAGAACTGTTGGA
TTGAAACCTCGTTAGGTTTTCATACTTCTTGTCGCACGTACTCAGAAATGATTGGGAATCTTGCTAAAGATGCTCTTTCT
GCAAAAAAATACCACCATTTCATCCGCCTTATGGGGCAGCAGGCCTCTTATACTACTTTAGAATGCGGATTGCAGACCCT
CCCTAATATAGCTTTAATTAGCGAACTTATTGCCACTAGGAAAATCTCTTTAAAGCAACTGAGTGAACAGCTTGCTTTAG
GTTTGGTACGCCGCTATAAATCTGGAAAAAACTATAGTACTGTGTTAATCCCAGAGGGACTGATTGAGCATATTTTCGAT
ACACGCAAGCTTATAGATGAACTCAATGTTTTACTCGCCAATGGAGATTCTTCTATTGAAAAAATTCTTTCCAAGCTCTC
TCCAGAGACATTAAAAACATTTCACTTGTTTCCTAAGGATATTGCGAACCAACTTCTTCTAGCTCGAGATTCTCATGGAA
ATGTTAGAGTTTCAAAAATTGCTACAGAAGAGCTGCTTGCAGTAATGGTAAAGAAGGAAATCGAAAAGATAAAACCCCAT
ATGGAATTCCATTCTGTATCGCATTTTTTTGGTTATGAAGCACGAGCAGGATTCCCTTCCAACTTTGATTGTAATTATGG
AATCGCTTTAGGGATCATTTCTGCGTTATTTCTGGTCCGACAAAAGACTGGTTATATGATTACAATCAATAATCTTGCTC
AATCTTATACTGAATGGCAAGGGGGAGCCACTCCATTATATAAGATGATGCACTTAGAAAATCGTTGTGGGACAGAGACT
CCTGTTATTAAAACAGATTCTGTAGACCCAAAATCCCCTGCTGTTCAACATTTACTTCAACAAAGTGATTCTTGTTTGGT
TGAAGATTTATACCGTTTTCCAGGCCCTTTACAGTACTTCGGGAAAGAGGAGCTTATAGATCAACGACCTTTGACCCTGC
TTTGGGAAAATCAAACGCATTCGCCTTTCCAGGCCCTTTACAGTACTTCGGGAAAGAGGAGCTTATAG

Upstream 100 bases:

>100_bases
TTATCCCGATGTCGATCACGCGTTTCCTTTTGCCGAATCTTCGGCTCTTTCGGATCTCACGCAATGGTTAAAAAGAGAAT
TAACTTCTGGGGAATAAACT

Downstream 100 bases:

>100_bases
ATCAACGACCTTTGACCCTGCTTTGGGAAAATCAAACGCATTCGCCTCTACTCTGACAACCTGTCCATTCCTATAAGAAT
ATTCTAAGCTAGATGCAAAG

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEVSIYTEQETSSKPLKIGVLLS
GGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRGLYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILN
TVKQLKLDGLLIIGGNNSNTDTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYKSGKNYSTVLIPEGLIEHIFD
TRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKDIANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPH
MEFHSVSHFFGYEARAGFPSNFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTHSPFQALYSTSGKRSL

Sequences:

>Translated_555_residues
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEVSIYTEQETSSKPLKIGVLLS
GGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRGLYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILN
TVKQLKLDGLLIIGGNNSNTDTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYKSGKNYSTVLIPEGLIEHIFD
TRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKDIANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPH
MEFHSVSHFFGYEARAGFPSNFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTHSPFQALYSTSGKRSL
>Mature_555_residues
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEVSIYTEQETSSKPLKIGVLLS
GGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRGLYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILN
TVKQLKLDGLLIIGGNNSNTDTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYKSGKNYSTVLIPEGLIEHIFD
TRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKDIANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPH
MEFHSVSHFFGYEARAGFPSNFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTHSPFQALYSTSGKRSL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=251, Percent_Identity=27.0916334661355, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 62416; Mature: 62416

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEV
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHCCCCC
SIYTEQETSSKPLKIGVLLSGGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRG
EEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHCCCHHHHHEECCCCHHHHHH
LYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILNTVKQLKLDGLLIIGGNNSNT
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
HHHHHHHHHHHCCCCCCEECCCCCCCCCHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHH
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYK
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKNYSTVLIPEGLIEHIFDTRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKD
CCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCHHHHHHHHCCHHH
IANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPHMEFHSVSHFFGYEARAGFPS
HHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
NFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTH
CEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCEEEECCCCC
SPFQALYSTSGKRSL
CHHHHHHHCCCCCCC
>Mature Secondary Structure
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEV
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHCCCCC
SIYTEQETSSKPLKIGVLLSGGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRG
EEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHCCCHHHHHEECCCCHHHHHH
LYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILNTVKQLKLDGLLIIGGNNSNT
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
HHHHHHHHHHHCCCCCCEECCCCCCCCCHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHH
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYK
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKNYSTVLIPEGLIEHIFDTRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKD
CCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCHHHHHHHHCCHHH
IANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPHMEFHSVSHFFGYEARAGFPS
HHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
NFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTH
CEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCEEEECCCCC
SPFQALYSTSGKRSL
CHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA