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The map label for this gene is rlmI [H]

Identifier: 15607037

GI number: 15607037

Start: 1457797

End: 1458972

Strand: Direct

Name: rlmI [H]

Synonym: aq_2071

Alternate gene names: 15607037

Gene position: 1457797-1458972 (Clockwise)

Preceding gene: 15607036

Following gene: 15607038

Centisome position: 93.97

GC content: 45.75

Gene sequence:

>1176_bases
ATGCTTCAAGTAAGAGTAAAACCCGGAGTAGAAGACAGGATTAGAGGATTTTTCCCTTGGGTTTACAGGTCGGAAATAGC
TTCCTTTTCAAGAAAACCAAAGAAAGGAGAGATAGTAGTCGTAAGGGACGTTAACGGAAAGTTTCTGGGCTACGGCTACA
TAAACCCTGAGGTAAACATAGCAATAAGAATTCTATCCTTTGACAAAAACGAGCCCGTAACTCCTGAACTTATCAGAAAG
AGAATAAAGCAGGCCTACGAATATAGAAAGAGACTTTACATAAACAGCAACGCTTACAGGCTTGTGCACTCGGAGGGGGA
TCTCCTCCCTGGACTTATCGTTGACGTTTACGGGAGTTATCTGGCGGTTGAGTTTACGACTTACGGTATGAACCAGTTCA
GAGAAGTAATACTGGACACACTCGTGGAACTACTAAGACCTAAAGGCATATACGAGAAGGTCAATGAAATCGCAAAGAGA
GTGGAAGGCTTGGACGTAGAGGAAAGGGTTATTTACGGAGAAGTTCCCGAAGAGGTCATAATCTGGGAACACGACCTCAA
GTACTACATAAATATACCTCAGGGACAAAAAACGGGATTTTTCCTAGATCAGAGAAATGCGAGGAAGTTTGTGAGAAACC
TGGTTGAACCAGGAGACAGGTGTCTGGACGTATTCTGTCACACGGGCGGATTTGCCCTGAACATGAAAAGGGCCGGAGCG
GGAGAGGTTATAGGAGTAGACATATCCGAGCTCGCCTTAAAGGAGGCGGAAAAGAACGCGAGACTCAACGGCTTTACGGA
CATAAAGTGGGTAAAGGCAAACGCTTTTGATTACCTGAGGGAACTCGACAAGAAGGGAGAGAAGTTTGACGTAATAATCA
TAGACCCTCCTTCTTTTGCGAAAAACAGGGCGGCTGTCCCGAACGCCTTGAGGGGATATAAAGAACTCTGTGTAAGAGGG
CTGAAGATAGCAAAGAGCGGGGGTTACCTCGCTATATTTTCCTGCTCCTTTCACATAACCGAACAGCACCTCCTTGACGT
TTTACTTCAGGCTTCCTACGACGTAAGGAGACAGGTAAGGGTAATAGCAAAGACCTTTCAGGACCTGGACCACCCGTGGA
TACTCCAGATGCCAAATACCTTATACTTAAAAGGCGTGTGGGTGGAAGTGGTATGA

Upstream 100 bases:

>100_bases
ATAGAAGAATTAAAGGAGGGTCTCTGGAAGAAGTACGAGGAAATAAGAGACAAAGAATCCGCGCAAGTAACCTGAACCTT
TTGATGTAGTTTATATAAAT

Downstream 100 bases:

>100_bases
AGAAGCACCACTGGGCACTCGTTTCCTTCGGCTTCAACATTTTCCAATCCCTCATAAAGCTTGTAGGAGGGCTCCTGACG
GGCTCCCTTTCACTTATCGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLQVRVKPGVEDRIRGFFPWVYRSEIASFSRKPKKGEIVVVRDVNGKFLGYGYINPEVNIAIRILSFDKNEPVTPELIRK
RIKQAYEYRKRLYINSNAYRLVHSEGDLLPGLIVDVYGSYLAVEFTTYGMNQFREVILDTLVELLRPKGIYEKVNEIAKR
VEGLDVEERVIYGEVPEEVIIWEHDLKYYINIPQGQKTGFFLDQRNARKFVRNLVEPGDRCLDVFCHTGGFALNMKRAGA
GEVIGVDISELALKEAEKNARLNGFTDIKWVKANAFDYLRELDKKGEKFDVIIIDPPSFAKNRAAVPNALRGYKELCVRG
LKIAKSGGYLAIFSCSFHITEQHLLDVLLQASYDVRRQVRVIAKTFQDLDHPWILQMPNTLYLKGVWVEVV

Sequences:

>Translated_391_residues
MLQVRVKPGVEDRIRGFFPWVYRSEIASFSRKPKKGEIVVVRDVNGKFLGYGYINPEVNIAIRILSFDKNEPVTPELIRK
RIKQAYEYRKRLYINSNAYRLVHSEGDLLPGLIVDVYGSYLAVEFTTYGMNQFREVILDTLVELLRPKGIYEKVNEIAKR
VEGLDVEERVIYGEVPEEVIIWEHDLKYYINIPQGQKTGFFLDQRNARKFVRNLVEPGDRCLDVFCHTGGFALNMKRAGA
GEVIGVDISELALKEAEKNARLNGFTDIKWVKANAFDYLRELDKKGEKFDVIIIDPPSFAKNRAAVPNALRGYKELCVRG
LKIAKSGGYLAIFSCSFHITEQHLLDVLLQASYDVRRQVRVIAKTFQDLDHPWILQMPNTLYLKGVWVEVV
>Mature_391_residues
MLQVRVKPGVEDRIRGFFPWVYRSEIASFSRKPKKGEIVVVRDVNGKFLGYGYINPEVNIAIRILSFDKNEPVTPELIRK
RIKQAYEYRKRLYINSNAYRLVHSEGDLLPGLIVDVYGSYLAVEFTTYGMNQFREVILDTLVELLRPKGIYEKVNEIAKR
VEGLDVEERVIYGEVPEEVIIWEHDLKYYINIPQGQKTGFFLDQRNARKFVRNLVEPGDRCLDVFCHTGGFALNMKRAGA
GEVIGVDISELALKEAEKNARLNGFTDIKWVKANAFDYLRELDKKGEKFDVIIIDPPSFAKNRAAVPNALRGYKELCVRG
LKIAKSGGYLAIFSCSFHITEQHLLDVLLQASYDVRRQVRVIAKTFQDLDHPWILQMPNTLYLKGVWVEVV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=378, Percent_Identity=33.5978835978836, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1787181, Length=236, Percent_Identity=27.9661016949153, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44984; Mature: 44984

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQVRVKPGVEDRIRGFFPWVYRSEIASFSRKPKKGEIVVVRDVNGKFLGYGYINPEVNI
CEEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCEEE
AIRILSFDKNEPVTPELIRKRIKQAYEYRKRLYINSNAYRLVHSEGDLLPGLIVDVYGSY
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHHHHHCCCE
LAVEFTTYGMNQFREVILDTLVELLRPKGIYEKVNEIAKRVEGLDVEERVIYGEVPEEVI
EEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCEEE
IWEHDLKYYINIPQGQKTGFFLDQRNARKFVRNLVEPGDRCLDVFCHTGGFALNMKRAGA
EEEECCEEEEECCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCC
GEVIGVDISELALKEAEKNARLNGFTDIKWVKANAFDYLRELDKKGEKFDVIIIDPPSFA
CCEEECCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCC
KNRAAVPNALRGYKELCVRGLKIAKSGGYLAIFSCSFHITEQHLLDVLLQASYDVRRQVR
CCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCHHHHHHHHHHCCHHHHHHHH
VIAKTFQDLDHPWILQMPNTLYLKGVWVEVV
HHHHHHHHCCCCEEEECCCEEEEEEEEEEEC
>Mature Secondary Structure
MLQVRVKPGVEDRIRGFFPWVYRSEIASFSRKPKKGEIVVVRDVNGKFLGYGYINPEVNI
CEEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCEEE
AIRILSFDKNEPVTPELIRKRIKQAYEYRKRLYINSNAYRLVHSEGDLLPGLIVDVYGSY
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHHHHHCCCE
LAVEFTTYGMNQFREVILDTLVELLRPKGIYEKVNEIAKRVEGLDVEERVIYGEVPEEVI
EEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCEEE
IWEHDLKYYINIPQGQKTGFFLDQRNARKFVRNLVEPGDRCLDVFCHTGGFALNMKRAGA
EEEECCEEEEECCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCC
GEVIGVDISELALKEAEKNARLNGFTDIKWVKANAFDYLRELDKKGEKFDVIIIDPPSFA
CCEEECCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCC
KNRAAVPNALRGYKELCVRGLKIAKSGGYLAIFSCSFHITEQHLLDVLLQASYDVRRQVR
CCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCHHHHHHHHHHCCHHHHHHHH
VIAKTFQDLDHPWILQMPNTLYLKGVWVEVV
HHHHHHHHCCCCEEEECCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA