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The map label for this gene is hisIE

Identifier: 15606967

GI number: 15606967

Start: 1385746

End: 1386363

Strand: Direct

Name: hisIE

Synonym: aq_1968

Alternate gene names: 15606967

Gene position: 1385746-1386363 (Clockwise)

Preceding gene: 15606966

Following gene: 15606968

Centisome position: 89.33

GC content: 44.34

Gene sequence:

>618_bases
ATGCTTAAACTCAAGTTCAACGAAGAAGGACTCATTCCCGTAATCGCTCAGGACTACAGAACGGGCGAAGTAAGAATGCT
CGCCTACGCAAACGAAGAAGCTATAAAAAAGACTCTGGAGACGGGATACGCCCATTATTACTCAAGGAGCAGGAAAAAGA
TATGGAAAAAAGGGGAGACCTCGGGAGAACTCCAGAAGGTAATTGAAGTAAGAGTGGACTGCGACGAAGATGCACTAATC
TACGTAATAGAGCAAGAAAAGGACAGGGCATGTCACACGGGTGAGAGAAACTGCTTCTTCAGAGACATAGAAGGAAACAA
AGTGGAAAAACCACTTCCCTTTGAAGTACTTCCCAGATTACAGGACGTGATAAGGGAAAAAATAGAAAGAAAGGAAGAAA
ACTCTTACACTGCAAAGTTGGTAAGCCAAGGGAAGGAAAGAGTGTTCCAGAAGTTCGGAGAAGAAGCCGTGGAAACCCTG
ATAGCCTTAATGAAAGGAGAAAAGGAAGAGGTGATATACGAGAGTGCGGATATGCTGTACACCTTTCTCGTATCCCTGAG
CGTAAGCGGGATAGACATAAAGGAGGTGATGGAGGAACTCATAAGACGCTTTAAGTGA

Upstream 100 bases:

>100_bases
AAATCCATGAAAACGGCGGGAAAGATAATACTGAAAGAGGTTCCCGTGGAAGTAGAAAGCGTTATAAACGAAAGGTGGAC
GAAAGATTAATATAATCCTT

Downstream 100 bases:

>100_bases
CGCCTGCGGGCTCTTTTGCCCTCTCTCCCAGTGCCAGCCACCTGTAATCCACGTTGAATCGTTTCGCTATGAATACGAGC
GTAGCGTCACAAGGTCTGGA

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MLKLKFNEEGLIPVIAQDYRTGEVRMLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGETSGELQKVIEVRVDCDEDALI
YVIEQEKDRACHTGERNCFFRDIEGNKVEKPLPFEVLPRLQDVIREKIERKEENSYTAKLVSQGKERVFQKFGEEAVETL
IALMKGEKEEVIYESADMLYTFLVSLSVSGIDIKEVMEELIRRFK

Sequences:

>Translated_205_residues
MLKLKFNEEGLIPVIAQDYRTGEVRMLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGETSGELQKVIEVRVDCDEDALI
YVIEQEKDRACHTGERNCFFRDIEGNKVEKPLPFEVLPRLQDVIREKIERKEENSYTAKLVSQGKERVFQKFGEEAVETL
IALMKGEKEEVIYESADMLYTFLVSLSVSGIDIKEVMEELIRRFK
>Mature_205_residues
MLKLKFNEEGLIPVIAQDYRTGEVRMLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGETSGELQKVIEVRVDCDEDALI
YVIEQEKDRACHTGERNCFFRDIEGNKVEKPLPFEVLPRLQDVIREKIERKEENSYTAKLVSQGKERVFQKFGEEAVETL
IALMKGEKEEVIYESADMLYTFLVSLSVSGIDIKEVMEELIRRFK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=197, Percent_Identity=39.0862944162437, Blast_Score=149, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319819, Length=226, Percent_Identity=31.4159292035398, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_AQUAE (O67780)

Other databases:

- EMBL:   AE000657
- PIR:   H70468
- RefSeq:   NP_214349.1
- ProteinModelPortal:   O67780
- GeneID:   1193677
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1968
- NMPDR:   fig|224324.1.peg.1364
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- PhylomeDB:   O67780
- ProtClustDB:   PRK02759
- BioCyc:   AAEO224324:AQ_1968-MONOMER
- BRENDA:   3.5.4.19
- BRENDA:   3.6.1.31
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 23944; Mature: 23944

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLKFNEEGLIPVIAQDYRTGEVRMLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGET
CEEEEECCCCCCEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGELQKVIEVRVDCDEDALIYVIEQEKDRACHTGERNCFFRDIEGNKVEKPLPFEVLPRL
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
QDVIREKIERKEENSYTAKLVSQGKERVFQKFGEEAVETLIALMKGEKEEVIYESADMLY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TFLVSLSVSGIDIKEVMEELIRRFK
HHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLKLKFNEEGLIPVIAQDYRTGEVRMLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGET
CEEEEECCCCCCEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGELQKVIEVRVDCDEDALIYVIEQEKDRACHTGERNCFFRDIEGNKVEKPLPFEVLPRL
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
QDVIREKIERKEENSYTAKLVSQGKERVFQKFGEEAVETLIALMKGEKEEVIYESADMLY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TFLVSLSVSGIDIKEVMEELIRRFK
HHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320