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The map label for this gene is dnaN

Identifier: 15606912

GI number: 15606912

Start: 1325853

End: 1326944

Strand: Direct

Name: dnaN

Synonym: aq_1882

Alternate gene names: 15606912

Gene position: 1325853-1326944 (Clockwise)

Preceding gene: 15606911

Following gene: 15606913

Centisome position: 85.47

GC content: 46.25

Gene sequence:

>1092_bases
ATGCGCGTTAAGGTGGACAGGGAGGAGCTTGAAGAGGTTCTTAAAAAAGCAAGAGAAAGCACGGAAAAAAAAGCCGCACT
CCCGATACTCGCGAACTTCTTACTCTCCGCAAAAGAGGAAAACTTAATCGTAAGGGCAACGGACTTGGAAAACTACCTTG
TAGTCTCCGTAAAGGGGGAGGTTGAAGAGGAAGGAGAGGTTTGCGTCCACTCTCAAAAACTCTACGATATAGTCAAGAAC
TTAAATTCCGCTTACGTTTACCTTCATACGGAAGGTGAAAAACTCGTCATAACGGGAGGAAAGAGTACGTACAAACTTCC
GACAGCTCCCGCGGAGGACTTTCCCGAATTTCCAGAAATCGTAGAAGGAGGAGAAACACTTTCGGGAAACCTTCTCGTTA
ACGGAATAGAAAAGGTAGAGTACGCCATAGCGAAGGAAGAAGCGAACATAGCCCTTCAGGGAATGTATCTGAGAGGATAC
GAGGACAGAATTCACTTTGTAGGTTCGGACGGTCACAGGCTTGCACTTTATGAACCTCTAGGAGAGTTTTCCAAGGAACT
CCTCATACCCAGAAAGAGCTTAAAGGTTTTAAAGAAGCTAATAACGGGCATAGAGGACGTAAACATTGAAAAGAGTGAAG
ACGAGTCTTTTGCTTACTTCTCCACTCCCGAGTGGAAACTCGCCGTTAGACTCCTGGAAGGAGAATTCCCGGACTACATG
AGTGTCATCCCTGAGGAGTTTTCGGCGGAAGTCTTGTTTGAGACAGAGGAAGTCTTAAAGGTTTTAAAGAGGTTGAAGGC
TTTAAGCGAAGGAAAAGTTTTTCCCGTGAAGATTACCTTAAGCGAAAACCTTGCCATCTTTGAGTTCGCGGATCCGGAGT
TCGGAGAAGCGAGAGAGGAAATTGAAGTGGAGTACACGGGAGAGCCCTTTGAGATAGGATTCAACGGAAAGTACCTTATG
GAGGCGCTTGACGCCTACGACAGCGAAAGAGTGTGGTTCAAGTTCACAACCCCCGACACGGCCACTTTATTGGAGGCTGA
AGATTACGAAAAGGAACCTTACAAGTGCATAATAATGCCGATGAGGGTGTAG

Upstream 100 bases:

>100_bases
GGCGGTTCAAAACGCAAATTTAATGTTCGGGCTTGCGGAAGACGACGGGCTCCTTCAACTTCCCGTTTTCCCCTGAAAGT
TTGTTATACTAATTCTCCAA

Downstream 100 bases:

>100_bases
CCATGAAAAAAGCTTTAATCTTTTTATTGAGCTTGAGCCTTTTAATTCCTGCGTTTAGCGAAGCCAAACCCAAGTCTTCA
AAGAAGAAGTACTACACTTA

Product: DNA polymerase III beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVKVDREELEEVLKKARESTEKKAALPILANFLLSAKEENLIVRATDLENYLVVSVKGEVEEEGEVCVHSQKLYDIVKN
LNSAYVYLHTEGEKLVITGGKSTYKLPTAPAEDFPEFPEIVEGGETLSGNLLVNGIEKVEYAIAKEEANIALQGMYLRGY
EDRIHFVGSDGHRLALYEPLGEFSKELLIPRKSLKVLKKLITGIEDVNIEKSEDESFAYFSTPEWKLAVRLLEGEFPDYM
SVIPEEFSAEVLFETEEVLKVLKRLKALSEGKVFPVKITLSENLAIFEFADPEFGEAREEIEVEYTGEPFEIGFNGKYLM
EALDAYDSERVWFKFTTPDTATLLEAEDYEKEPYKCIIMPMRV

Sequences:

>Translated_363_residues
MRVKVDREELEEVLKKARESTEKKAALPILANFLLSAKEENLIVRATDLENYLVVSVKGEVEEEGEVCVHSQKLYDIVKN
LNSAYVYLHTEGEKLVITGGKSTYKLPTAPAEDFPEFPEIVEGGETLSGNLLVNGIEKVEYAIAKEEANIALQGMYLRGY
EDRIHFVGSDGHRLALYEPLGEFSKELLIPRKSLKVLKKLITGIEDVNIEKSEDESFAYFSTPEWKLAVRLLEGEFPDYM
SVIPEEFSAEVLFETEEVLKVLKRLKALSEGKVFPVKITLSENLAIFEFADPEFGEAREEIEVEYTGEPFEIGFNGKYLM
EALDAYDSERVWFKFTTPDTATLLEAEDYEKEPYKCIIMPMRV
>Mature_363_residues
MRVKVDREELEEVLKKARESTEKKAALPILANFLLSAKEENLIVRATDLENYLVVSVKGEVEEEGEVCVHSQKLYDIVKN
LNSAYVYLHTEGEKLVITGGKSTYKLPTAPAEDFPEFPEIVEGGETLSGNLLVNGIEKVEYAIAKEEANIALQGMYLRGY
EDRIHFVGSDGHRLALYEPLGEFSKELLIPRKSLKVLKKLITGIEDVNIEKSEDESFAYFSTPEWKLAVRLLEGEFPDYM
SVIPEEFSAEVLFETEEVLKVLKRLKALSEGKVFPVKITLSENLAIFEFADPEFGEAREEIEVEYTGEPFEIGFNGKYLM
EALDAYDSERVWFKFTTPDTATLLEAEDYEKEPYKCIIMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=370, Percent_Identity=28.9189189189189, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_AQUAE (O67725)

Other databases:

- EMBL:   AE000657
- PIR:   C70462
- RefSeq:   NP_214293.1
- ProteinModelPortal:   O67725
- GeneID:   1193532
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1882
- NMPDR:   fig|224324.1.peg.1308
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- PhylomeDB:   O67725
- ProtClustDB:   CLSK2299859
- BioCyc:   AAEO224324:AQ_1882-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 41301; Mature: 41301

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKVDREELEEVLKKARESTEKKAALPILANFLLSAKEENLIVRATDLENYLVVSVKGE
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECC
VEEEGEVCVHSQKLYDIVKNLNSAYVYLHTEGEKLVITGGKSTYKLPTAPAEDFPEFPEI
CCCCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCHHH
VEGGETLSGNLLVNGIEKVEYAIAKEEANIALQGMYLRGYEDRIHFVGSDGHRLALYEPL
HCCCCEEECCEEEECHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCEEEEECCH
GEFSKELLIPRKSLKVLKKLITGIEDVNIEKSEDESFAYFSTPEWKLAVRLLEGEFPDYM
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCHHH
SVIPEEFSAEVLFETEEVLKVLKRLKALSEGKVFPVKITLSENLAIFEFADPEFGEAREE
HHCCHHCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCE
IEVEYTGEPFEIGFNGKYLMEALDAYDSERVWFKFTTPDTATLLEAEDYEKEPYKCIIMP
EEEEECCCCEEECCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEEE
MRV
ECC
>Mature Secondary Structure
MRVKVDREELEEVLKKARESTEKKAALPILANFLLSAKEENLIVRATDLENYLVVSVKGE
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECC
VEEEGEVCVHSQKLYDIVKNLNSAYVYLHTEGEKLVITGGKSTYKLPTAPAEDFPEFPEI
CCCCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCHHH
VEGGETLSGNLLVNGIEKVEYAIAKEEANIALQGMYLRGYEDRIHFVGSDGHRLALYEPL
HCCCCEEECCEEEECHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCEEEEECCH
GEFSKELLIPRKSLKVLKKLITGIEDVNIEKSEDESFAYFSTPEWKLAVRLLEGEFPDYM
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCHHH
SVIPEEFSAEVLFETEEVLKVLKRLKALSEGKVFPVKITLSENLAIFEFADPEFGEAREE
HHCCHHCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCE
IEVEYTGEPFEIGFNGKYLMEALDAYDSERVWFKFTTPDTATLLEAEDYEKEPYKCIIMP
EEEEECCCCEEECCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEEE
MRV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320