Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 15606908

GI number: 15606908

Start: 1323017

End: 1323901

Strand: Direct

Name: mraW

Synonym: aq_1875

Alternate gene names: 15606908

Gene position: 1323017-1323901 (Clockwise)

Preceding gene: 15606907

Following gene: 15606909

Centisome position: 85.28

GC content: 43.62

Gene sequence:

>885_bases
ATGGAAGTTCTCCACAAACCTGTACTTTTAAGGGAAAGTGTTGAGTTTCTGACCGAAAACGGAGGAAAGATTTACGTTGA
CTGCACGCTCGGAGGTGGAGGGCACGCAAAGGAAATTCTGAAGAAAAGGAAAGATATTTTCCTTATCGGAATAGACAGGG
ACGAGGAAGCTATAGAAATAGCAAAGGAAAATTTAAAAGAGTTTGAGGGGAGGTTCAGTATATACAAGGCGAACTTCAAG
GATTTGGACCTGGTTCTGAAAGAGGAAGGTATAGACAAAGTAGACGGATTTCTCTTTGACCTCGGAGTTTCCATGTACCA
GCTAAAGGGAGAAAGGGGGTTTACCTTTCAGGAGGACCAGCCCCTTGATATGCGTATGGACAAAGAGCAAACGTTAACCG
CTTGCAAAGTCGTAAACAACTACCCCGAAAGGGAGTTAATAAGGATACTCAAAGAGTACGGCGAAGAGAAGTTTGCGGTA
AGGATTGCAAAGGCGATAGTCCAGAGGAGGAAGAAAAAGCCAATAGAAACGACGGGAGAACTCGTTGACGTAATCCTTTC
CGTGGTTCCCGAGTACTACAAACACGGCAAGATACACCCCGCCACGAGAACCTTTCAGGCGATAAGGATTGAGGTAAATA
AAGAACTGGAATCCCTCAAAGAAGCTTTGGAAAAAACACCTTATCACTTTAACTCAAAAGGCAGACTCGTAGTCATTACT
TTCCACTCCCTTGAGGACAGGATAGTTAAGCACTTTATAAAGGAGAAAGAAAAGGAAGGAATTTTTAGGATAATTACGAA
GAAGCCTGTAACGCCGTCCGAAGAAGAAATCAGGGAAAATCCAGCCTCGCGGAGTGCGAAACTTCGTGTAGCTGAGAAAA
TTTAA

Upstream 100 bases:

>100_bases
AGCCTTTGCGGAAGTGGGCGTTGCAAAGATAATAAGGGAAGAGACTAAGAGGATAGTCGAGGAAAAAATAAAGGAATTGC
TGAAGTAAATTTAAATTCTT

Downstream 100 bases:

>100_bases
TGTTATAATTACAATCTTGCTTTAAACTCGGAAGAGGTGAATGGGATGAAGACATACAGGATTAAGCCCGAAGAAGTGGA
AAGAAAGTGGTGGGTAGTGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEVLHKPVLLRESVEFLTENGGKIYVDCTLGGGGHAKEILKKRKDIFLIGIDRDEEAIEIAKENLKEFEGRFSIYKANFK
DLDLVLKEEGIDKVDGFLFDLGVSMYQLKGERGFTFQEDQPLDMRMDKEQTLTACKVVNNYPERELIRILKEYGEEKFAV
RIAKAIVQRRKKKPIETTGELVDVILSVVPEYYKHGKIHPATRTFQAIRIEVNKELESLKEALEKTPYHFNSKGRLVVIT
FHSLEDRIVKHFIKEKEKEGIFRIITKKPVTPSEEEIRENPASRSAKLRVAEKI

Sequences:

>Translated_294_residues
MEVLHKPVLLRESVEFLTENGGKIYVDCTLGGGGHAKEILKKRKDIFLIGIDRDEEAIEIAKENLKEFEGRFSIYKANFK
DLDLVLKEEGIDKVDGFLFDLGVSMYQLKGERGFTFQEDQPLDMRMDKEQTLTACKVVNNYPERELIRILKEYGEEKFAV
RIAKAIVQRRKKKPIETTGELVDVILSVVPEYYKHGKIHPATRTFQAIRIEVNKELESLKEALEKTPYHFNSKGRLVVIT
FHSLEDRIVKHFIKEKEKEGIFRIITKKPVTPSEEEIRENPASRSAKLRVAEKI
>Mature_294_residues
MEVLHKPVLLRESVEFLTENGGKIYVDCTLGGGGHAKEILKKRKDIFLIGIDRDEEAIEIAKENLKEFEGRFSIYKANFK
DLDLVLKEEGIDKVDGFLFDLGVSMYQLKGERGFTFQEDQPLDMRMDKEQTLTACKVVNNYPERELIRILKEYGEEKFAV
RIAKAIVQRRKKKPIETTGELVDVILSVVPEYYKHGKIHPATRTFQAIRIEVNKELESLKEALEKTPYHFNSKGRLVVIT
FHSLEDRIVKHFIKEKEKEGIFRIITKKPVTPSEEEIRENPASRSAKLRVAEKI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=35.8823529411765, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=39.1959798994975, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=42.3948220064725, Blast_Score=214, Evalue=7e-57,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=38.109756097561, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=38.109756097561, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_AQUAE (O67721)

Other databases:

- EMBL:   AE000657
- PIR:   G70461
- RefSeq:   NP_214289.1
- ProteinModelPortal:   O67721
- SMR:   O67721
- GeneID:   1193528
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1875
- NMPDR:   fig|224324.1.peg.1304
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   O67721
- ProtClustDB:   PRK00050
- BioCyc:   AAEO224324:AQ_1875-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34020; Mature: 34020

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVLHKPVLLRESVEFLTENGGKIYVDCTLGGGGHAKEILKKRKDIFLIGIDRDEEAIEI
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCCHHHHHH
AKENLKEFEGRFSIYKANFKDLDLVLKEEGIDKVDGFLFDLGVSMYQLKGERGFTFQEDQ
HHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCHHHEECCCCCCCCCCCC
PLDMRMDKEQTLTACKVVNNYPERELIRILKEYGEEKFAVRIAKAIVQRRKKKPIETTGE
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
LVDVILSVVPEYYKHGKIHPATRTFQAIRIEVNKELESLKEALEKTPYHFNSKGRLVVIT
HHHHHHHHHHHHHHCCCCCCHHHHEEHHEEEHHHHHHHHHHHHHHCCCEECCCCCEEEEE
FHSLEDRIVKHFIKEKEKEGIFRIITKKPVTPSEEEIRENPASRSAKLRVAEKI
ECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCCCEEEECCCC
>Mature Secondary Structure
MEVLHKPVLLRESVEFLTENGGKIYVDCTLGGGGHAKEILKKRKDIFLIGIDRDEEAIEI
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCCHHHHHH
AKENLKEFEGRFSIYKANFKDLDLVLKEEGIDKVDGFLFDLGVSMYQLKGERGFTFQEDQ
HHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCHHHEECCCCCCCCCCCC
PLDMRMDKEQTLTACKVVNNYPERELIRILKEYGEEKFAVRIAKAIVQRRKKKPIETTGE
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
LVDVILSVVPEYYKHGKIHPATRTFQAIRIEVNKELESLKEALEKTPYHFNSKGRLVVIT
HHHHHHHHHHHHHHCCCCCCHHHHEEHHEEEHHHHHHHHHHHHHHCCCEECCCCCEEEEE
FHSLEDRIVKHFIKEKEKEGIFRIITKKPVTPSEEEIRENPASRSAKLRVAEKI
ECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320