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The map label for this gene is pmbA [H]

Identifier: 15606906

GI number: 15606906

Start: 1319839

End: 1321164

Strand: Direct

Name: pmbA [H]

Synonym: aq_1871

Alternate gene names: 15606906

Gene position: 1319839-1321164 (Clockwise)

Preceding gene: 15606905

Following gene: 15606907

Centisome position: 85.08

GC content: 43.82

Gene sequence:

>1326_bases
ATGGATAACTTAGAAAACCTCGTCAAAAAATACATAAAACCCGGATACGAGTACGAAGTATTCTTTGAAAGGGTTAAAAA
GTTAAAGATAGAAGTCTCAAATGAGCAAGTTGAAAACGTCTCTTCTTCCGAAGAACGGGGTATAGGAATAAGGGTTTTAA
AAGATAAACGCCTTGGATTTTCCTACACTTCTTTTCTCGGTGAGGAAGAGATAAAGGACACAGTTGAAAAAGCCATGGAA
ATGTGTGAAATTCAGGAGCCTGATGAGGCAAACGGCTTTATAGAGAAACTGAAACCCTCTAAAGCGGTCTCCGTTTACGA
CGAAGAGTCCCTGTCAATTCCCTTACAGGAAAAGATGGAAATACCCGTCAAGATGGAAAAGTACGCAAAGGAACTGGACA
AGAGGATTGTAGGAGTGAGAAAGTCCACCCTCACCGAGGTTGAGTTTGAAGTAAGGAGCTTTAACTCTTTTGGTGTTGAG
TTCGGATATAGGGGAACCTCTTACACCTCTATGATAGCGACACTCGGAACTGAAAATGATGACAGCGCAATCTCTTGGGA
ATTCAGAGGCGCAAGGAGACTGAAGAACTTAAACTGGAAGTCCATGGTTGAGGACGCTGTCTTCAAAACCGTAAACCTCC
TTCACCCATCTCCCTTTGAAACTAGAAGTATGCCCGTTGTGTTCTTCAGGGAAAGCTTTGCGATGCTCCTTGACGCCTTC
AGCCCTATGTTTTTGGGAGACTACCTGGTTAAAGGAAAAACACTTTTAAAGGATAAGGTAAACGAAATAGTAGCGAGTGA
AAAAATCACGCTCGTTGATGACGGCTCCATGGAAGAGGGGTTTTCCACTTTCCCTTACGACGCGGAAGGAGTGCCCACCA
GAAAAAATTTATTAATAGATAAAGGAGTGTTTAAGGGCTTTCTGCACAGCCTCTACACCGCGAGGAAGTCCTCTCAGGAA
CCTACCGGAAACTCCGTAAGAGGATCCTACAAAGAACTGCCATCTTCGGGAACCACGAACTTTTACTTAGAAAAGGGGAA
CCTTTCCTTTGAAGAATTACTCTCCTATTACGATGAGGTTTTCTTGGTTCTGGAGGTAATGGGACTCCACACCGTTGACA
CTATTTCGGGGGACTTCTCCCTTGGTTGTTCGGGAATTCTTTACAAAAAGGGAAAGAAGGACAAAACGGTAAGGGGCATA
ACTGTAGCGGGGAATATACTAGATCTACTTAAGAACGTTGAAGAAGTGGGGAACGATCTCACTTTTTATGGGAGTGTAGG
TTCACCCTCGGTTCTCGTTAAAAAATTAACCCTCGGAGGGGGGTGA

Upstream 100 bases:

>100_bases
CAGACCCAAAACTCTAAAAGAAAGATTAAAAGAAGAGAGACTGAGAAAGAGGAAATTAAAAGCGAAGAAGAAAGAACAGG
AGTGAGCTAAAATTCTACGT

Downstream 100 bases:

>100_bases
TGAAAAAATTAATCCTCTTACTACTCTGGATTTTTCTATTAACCTTTTCCCTTTCATCTTTCTACATTTCCTACTCCGCA
TTCAACGAAGAGAGGACGCA

Product: peptide maturation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MDNLENLVKKYIKPGYEYEVFFERVKKLKIEVSNEQVENVSSSEERGIGIRVLKDKRLGFSYTSFLGEEEIKDTVEKAME
MCEIQEPDEANGFIEKLKPSKAVSVYDEESLSIPLQEKMEIPVKMEKYAKELDKRIVGVRKSTLTEVEFEVRSFNSFGVE
FGYRGTSYTSMIATLGTENDDSAISWEFRGARRLKNLNWKSMVEDAVFKTVNLLHPSPFETRSMPVVFFRESFAMLLDAF
SPMFLGDYLVKGKTLLKDKVNEIVASEKITLVDDGSMEEGFSTFPYDAEGVPTRKNLLIDKGVFKGFLHSLYTARKSSQE
PTGNSVRGSYKELPSSGTTNFYLEKGNLSFEELLSYYDEVFLVLEVMGLHTVDTISGDFSLGCSGILYKKGKKDKTVRGI
TVAGNILDLLKNVEEVGNDLTFYGSVGSPSVLVKKLTLGGG

Sequences:

>Translated_441_residues
MDNLENLVKKYIKPGYEYEVFFERVKKLKIEVSNEQVENVSSSEERGIGIRVLKDKRLGFSYTSFLGEEEIKDTVEKAME
MCEIQEPDEANGFIEKLKPSKAVSVYDEESLSIPLQEKMEIPVKMEKYAKELDKRIVGVRKSTLTEVEFEVRSFNSFGVE
FGYRGTSYTSMIATLGTENDDSAISWEFRGARRLKNLNWKSMVEDAVFKTVNLLHPSPFETRSMPVVFFRESFAMLLDAF
SPMFLGDYLVKGKTLLKDKVNEIVASEKITLVDDGSMEEGFSTFPYDAEGVPTRKNLLIDKGVFKGFLHSLYTARKSSQE
PTGNSVRGSYKELPSSGTTNFYLEKGNLSFEELLSYYDEVFLVLEVMGLHTVDTISGDFSLGCSGILYKKGKKDKTVRGI
TVAGNILDLLKNVEEVGNDLTFYGSVGSPSVLVKKLTLGGG
>Mature_441_residues
MDNLENLVKKYIKPGYEYEVFFERVKKLKIEVSNEQVENVSSSEERGIGIRVLKDKRLGFSYTSFLGEEEIKDTVEKAME
MCEIQEPDEANGFIEKLKPSKAVSVYDEESLSIPLQEKMEIPVKMEKYAKELDKRIVGVRKSTLTEVEFEVRSFNSFGVE
FGYRGTSYTSMIATLGTENDDSAISWEFRGARRLKNLNWKSMVEDAVFKTVNLLHPSPFETRSMPVVFFRESFAMLLDAF
SPMFLGDYLVKGKTLLKDKVNEIVASEKITLVDDGSMEEGFSTFPYDAEGVPTRKNLLIDKGVFKGFLHSLYTARKSSQE
PTGNSVRGSYKELPSSGTTNFYLEKGNLSFEELLSYYDEVFLVLEVMGLHTVDTISGDFSLGCSGILYKKGKKDKTVRGI
TVAGNILDLLKNVEEVGNDLTFYGSVGSPSVLVKKLTLGGG

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=430, Percent_Identity=25.8139534883721, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789640, Length=424, Percent_Identity=25.9433962264151, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49574; Mature: 49574

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLENLVKKYIKPGYEYEVFFERVKKLKIEVSNEQVENVSSSEERGIGIRVLKDKRLGF
CCCHHHHHHHHCCCCCCHHHHHHHHHHHEEEECHHHHHCCCCHHHCCCEEEEEECCCCCC
SYTSFLGEEEIKDTVEKAMEMCEIQEPDEANGFIEKLKPSKAVSVYDEESLSIPLQEKME
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHCC
IPVKMEKYAKELDKRIVGVRKSTLTEVEFEVRSFNSFGVEFGYRGTSYTSMIATLGTEND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCC
DSAISWEFRGARRLKNLNWKSMVEDAVFKTVNLLHPSPFETRSMPVVFFRESFAMLLDAF
CCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
SPMFLGDYLVKGKTLLKDKVNEIVASEKITLVDDGSMEEGFSTFPYDAEGVPTRKNLLID
CHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
KGVFKGFLHSLYTARKSSQEPTGNSVRGSYKELPSSGTTNFYLEKGNLSFEELLSYYDEV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
FLVLEVMGLHTVDTISGDFSLGCSGILYKKGKKDKTVRGITVAGNILDLLKNVEEVGNDL
HHHHHHHCCCCCHHCCCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCE
TFYGSVGSPSVLVKKLTLGGG
EEEECCCCHHHHHEECCCCCC
>Mature Secondary Structure
MDNLENLVKKYIKPGYEYEVFFERVKKLKIEVSNEQVENVSSSEERGIGIRVLKDKRLGF
CCCHHHHHHHHCCCCCCHHHHHHHHHHHEEEECHHHHHCCCCHHHCCCEEEEEECCCCCC
SYTSFLGEEEIKDTVEKAMEMCEIQEPDEANGFIEKLKPSKAVSVYDEESLSIPLQEKME
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHCC
IPVKMEKYAKELDKRIVGVRKSTLTEVEFEVRSFNSFGVEFGYRGTSYTSMIATLGTEND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCC
DSAISWEFRGARRLKNLNWKSMVEDAVFKTVNLLHPSPFETRSMPVVFFRESFAMLLDAF
CCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHH
SPMFLGDYLVKGKTLLKDKVNEIVASEKITLVDDGSMEEGFSTFPYDAEGVPTRKNLLID
CHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
KGVFKGFLHSLYTARKSSQEPTGNSVRGSYKELPSSGTTNFYLEKGNLSFEELLSYYDEV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
FLVLEVMGLHTVDTISGDFSLGCSGILYKKGKKDKTVRGITVAGNILDLLKNVEEVGNDL
HHHHHHHCCCCCHHCCCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCE
TFYGSVGSPSVLVKKLTLGGG
EEEECCCCHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]