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The map label for this gene is prfB

Identifier: 15606882

GI number: 15606882

Start: 1293461

End: 1294582

Strand: Direct

Name: prfB

Synonym: aq_1840

Alternate gene names: 15606882

Gene position: 1293461-1294582 (Clockwise)

Preceding gene: 15607140

Following gene: 15606891

Centisome position: 83.38

GC content: 45.72

Gene sequence:

>1122_bases
ATGATGATGGTAGAGTTAAAGGGTAAAGTAGAAGAACTTAGAAAACGGCTTGAAGACGTAAAGAAGATACTTTCTCCTGA
GAAACTTGAGAGTGAATTAAAAGAACTCGACCAGAAGATGTCAGAACCAAACTTCTGGGAGGATCAGGAAAAGGCCAAGC
AGGTAATACAGAGAAGGAAGTGGGTAGAGGAAACTCTGAATAAATTGAAAAACCTTGAAAAGAGTGTAAAGGATTTAGAG
GAGCTAGTGGAGATAACTTCCGAAGAGGACACGGAAACCTGGGCGATGATGGACGAAGAAATCAAAGAAGTGGAAAGGAC
TCTAAGAGAACTCGAACTAAAGACTTACCTTTCCGGAGAGATGGACGCCAAGAACGCGTATCTCACCATACAGGCGGGTG
CGGGAGGAACGGAAGCCTGTGACTGGGCCGATATGCTCTTCAGGATGTACAAGAGATGGGCTGAAAAGAAAGGGTACGAG
GTTGAACTCATAGACATAACTCCCGACGACGTAGCCGGTATAAAGAGCGTTACAGTCCTCGTAAAAGGTCCATACGCTTA
CGGATACCTGAAGGGAGAGCAGGGAGTTCACAGACTCGTGAGGATTTCTCCCTTCGACGCGAACGCAAGGAGACACACCT
CCTTTGCGGCAGTCTCCGTTATGCCCCAGATAGACGAGGACATAAAGATAGAAATAAAACCTGAGGACCTGAAGATAGAA
ACCTTCAGGGCTTCGGGAGCGGGAGGACAGTACGTAAACAAAACCGATACTGCGGTTAGGATTACACACATTCCCACAGG
CATAACAGTATCCTGTCAGCAAGAAAGGTCTCAGTACCAGAACAAGAGAAAAGCACTTGAACTTCTGAAGGCTAAACTCT
ATCAGCTCGAGATGAAGAAACTGGAAGAAAAGAAAAAGCAGTACGAAGGCGAGAAAACGGACATAGGCTGGGGTCACCAG
ATCAGATCCTACGTCTTTCACCCCTACAAACTAATAAAGGACCTCAGAACGGGTTACGAGACGGGAAACGTGGAAGCAGT
AATGGACGGAGAGATAGACGAGTTCATAGAAAGTTACCTAAAGTGGAAAGCTCAAAAGGAAAAAGAATCATCCAACAACT
AA

Upstream 100 bases:

>100_bases
AAAGGAAGTGCCCTTTGAAACCCTAAAGGAAGCTGTAGAAACTTGGGGATACAAGGTAGTGGAATAATTGAAAATAAATT
TCAATTGCAATAAATTATAA

Downstream 100 bases:

>100_bases
TACCTGCTTTCCTTCTGCACTCTCTTCAAAACCCTCCACGTAAAGGCTGAGCAGCTTAGCCCCCTCCACCAGAGCTTGAA
TTAAGACGGGATCCCTCAAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MMMVELKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEETLNKLKNLEKSVKDLE
ELVEITSEEDTETWAMMDEEIKEVERTLRELELKTYLSGEMDAKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYE
VELIDITPDDVAGIKSVTVLVKGPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQIDEDIKIEIKPEDLKIE
TFRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKKLEEKKKQYEGEKTDIGWGHQ
IRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYLKWKAQKEKESSNN

Sequences:

>Translated_373_residues
MMMVELKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEETLNKLKNLEKSVKDLE
ELVEITSEEDTETWAMMDEEIKEVERTLRELELKTYLSGEMDAKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYE
VELIDITPDDVAGIKSVTVLVKGPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQIDEDIKIEIKPEDLKIE
TFRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKKLEEKKKQYEGEKTDIGWGHQ
IRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYLKWKAQKEKESSNN
>Mature_373_residues
MMMVELKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEETLNKLKNLEKSVKDLE
ELVEITSEEDTETWAMMDEEIKEVERTLRELELKTYLSGEMDAKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYE
VELIDITPDDVAGIKSVTVLVKGPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQIDEDIKIEIKPEDLKIE
TFRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKKLEEKKKQYEGEKTDIGWGHQ
IRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYLKWKAQKEKESSNN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=34.5679012345679, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI34577120, Length=250, Percent_Identity=38, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI166795305, Length=227, Percent_Identity=33.920704845815, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI2367172, Length=357, Percent_Identity=47.6190476190476, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1787462, Length=294, Percent_Identity=38.0952380952381, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=292, Percent_Identity=33.2191780821918, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=34.5609065155807, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19921226, Length=302, Percent_Identity=35.0993377483444, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_AQUAE (O67695)

Other databases:

- EMBL:   AE000657
- PIR:   E70458
- RefSeq:   NP_214263.1
- ProteinModelPortal:   O67695
- SMR:   O67695
- GeneID:   1193502
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1840
- NMPDR:   fig|224324.1.peg.1278
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- PhylomeDB:   O67695
- ProtClustDB:   PRK00578
- BioCyc:   AAEO224324:AQ_1840-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 43310; Mature: 43310

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMVELKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRK
CEEEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
WVEETLNKLKNLEKSVKDLEELVEITSEEDTETWAMMDEEIKEVERTLRELELKTYLSGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MDAKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYEVELIDITPDDVAGIKSVTVL
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCEEEEE
VKGPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQIDEDIKIEIKPEDLKIE
EECCEEEEEEECCHHHHHHHEECCCCCCCHHCCHHHHEEECCCCCCCCEEEECCCCEEEE
TFRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKK
EEEECCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEKKKQYEGEKTDIGWGHQIRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
KWKAQKEKESSNN
HHHHHHHHCCCCC
>Mature Secondary Structure
MMMVELKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRK
CEEEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
WVEETLNKLKNLEKSVKDLEELVEITSEEDTETWAMMDEEIKEVERTLRELELKTYLSGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MDAKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYEVELIDITPDDVAGIKSVTVL
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCEEEEE
VKGPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQIDEDIKIEIKPEDLKIE
EECCEEEEEEECCHHHHHHHEECCCCCCCHHCCHHHHEEECCCCCCCCEEEECCCCEEEE
TFRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKK
EEEECCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEKKKQYEGEKTDIGWGHQIRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
KWKAQKEKESSNN
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320