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The map label for this gene is lytB

Identifier: 15606811

GI number: 15606811

Start: 1224574

End: 1225443

Strand: Direct

Name: lytB

Synonym: aq_1739

Alternate gene names: 15606811

Gene position: 1224574-1225443 (Clockwise)

Preceding gene: 15606809

Following gene: 15606812

Centisome position: 78.94

GC content: 44.94

Gene sequence:

>870_bases
ATGGTTGACATAATAATCGCAGAACACGCAGGTTTTTGCTTCGGTGTAAAGAGGGCTGTAAAACTCGCAGAAGAAAGCCT
AAAAGAATCCCAAGGAAAGGTTTATACACTCGGTCCTATAATACACAATCCCCAAGAGGTGAATAGACTAAAGAATCTCG
GCGTTTTTCCAAGTCAAGGGGAGGAGTTTAAAGAAGGGGATACGGTCATAATACGTTCCCACGGTATCCCTCCGGAAAAG
GAAGAAGCTTTAAGGAAGAAGGGTCTTAAAGTAATAGACGCTACCTGCCCATACGTTAAAGCAGTGCACGAGGCGGTCTG
TCAATTGACCAGGGAGGGTTATTTTGTGGTCCTCGTAGGAGAAAAGAATCACCCCGAGGTTATAGGGACACTCGGGTATT
TGAGGGCTTGCAACGGAAAGGGAATAGTCGTTGAAACTTTAGAGGACATAGGGGAAGCTTTAAAACACGAAAGAGTTGGA
ATAGTCGCTCAAACAACCCAGAACGAGGAGTTCTTTAAGGAAGTAGTCGGCGAGATAGCCCTGTGGGTTAAGGAAGTAAA
GGTAATAAACACCATATGCAACGCTACGTCCTTAAGGCAGGAGAGTGTAAAAAAGCTCGCTCCAGAGGTTGACGTGATGA
TAATAATCGGTGGAAAGAACAGCGGAAACACGAGGAGACTTTATTATATTTCTAAAGAATTAAATCCGAACACCTACCAT
ATAGAAACCGCTGAAGAACTCCAACCCGAGTGGTTCAGGGGAGTAAAAAGGGTGGGGATTTCCGCGGGAGCTTCAACGCC
GGACTGGATTATAGAGCAAGTAAAGAGTAGAATACAAGAGATATGCGAAGGGCAATTGGTTTCCTCCTGA

Upstream 100 bases:

>100_bases
AACGATCGCTTCTTTAATCATAAGAGCACCTCCCTCCCTTTAAAAATTTAGGCGGATAAATCTCATTGACATTTATGCAG
TCTTTGAATATTCTTTAGGC

Downstream 100 bases:

>100_bases
TAGTAATAACTTTCACTTTTGCTTCCCTTTACGAAGAATTTGTAAAGGAACAGGTAAAAGAACTTCCTTTGGACAAGGCT
GTGGTAGTCGGAACAGGTAA

Product: LytB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MVDIIIAEHAGFCFGVKRAVKLAEESLKESQGKVYTLGPIIHNPQEVNRLKNLGVFPSQGEEFKEGDTVIIRSHGIPPEK
EEALRKKGLKVIDATCPYVKAVHEAVCQLTREGYFVVLVGEKNHPEVIGTLGYLRACNGKGIVVETLEDIGEALKHERVG
IVAQTTQNEEFFKEVVGEIALWVKEVKVINTICNATSLRQESVKKLAPEVDVMIIIGGKNSGNTRRLYYISKELNPNTYH
IETAEELQPEWFRGVKRVGISAGASTPDWIIEQVKSRIQEICEGQLVSS

Sequences:

>Translated_289_residues
MVDIIIAEHAGFCFGVKRAVKLAEESLKESQGKVYTLGPIIHNPQEVNRLKNLGVFPSQGEEFKEGDTVIIRSHGIPPEK
EEALRKKGLKVIDATCPYVKAVHEAVCQLTREGYFVVLVGEKNHPEVIGTLGYLRACNGKGIVVETLEDIGEALKHERVG
IVAQTTQNEEFFKEVVGEIALWVKEVKVINTICNATSLRQESVKKLAPEVDVMIIIGGKNSGNTRRLYYISKELNPNTYH
IETAEELQPEWFRGVKRVGISAGASTPDWIIEQVKSRIQEICEGQLVSS
>Mature_289_residues
MVDIIIAEHAGFCFGVKRAVKLAEESLKESQGKVYTLGPIIHNPQEVNRLKNLGVFPSQGEEFKEGDTVIIRSHGIPPEK
EEALRKKGLKVIDATCPYVKAVHEAVCQLTREGYFVVLVGEKNHPEVIGTLGYLRACNGKGIVVETLEDIGEALKHERVG
IVAQTTQNEEFFKEVVGEIALWVKEVKVINTICNATSLRQESVKKLAPEVDVMIIIGGKNSGNTRRLYYISKELNPNTYH
IETAEELQPEWFRGVKRVGISAGASTPDWIIEQVKSRIQEICEGQLVSS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=291, Percent_Identity=32.6460481099656, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_AQUAE (O67625)

Other databases:

- EMBL:   AE000657
- PIR:   G70449
- RefSeq:   NP_214191.1
- PDB:   3DNF
- PDBsum:   3DNF
- GeneID:   1193340
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1739
- NMPDR:   fig|224324.1.peg.1206
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   O67625
- ProtClustDB:   CLSK2299815
- BioCyc:   AAEO224324:AQ_1739-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 32104; Mature: 32104

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIIIAEHAGFCFGVKRAVKLAEESLKESQGKVYTLGPIIHNPQEVNRLKNLGVFPSQG
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCCC
EEFKEGDTVIIRSHGIPPEKEEALRKKGLKVIDATCPYVKAVHEAVCQLTREGYFVVLVG
CCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEEE
EKNHPEVIGTLGYLRACNGKGIVVETLEDIGEALKHERVGIVAQTTQNEEFFKEVVGEIA
CCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
LWVKEVKVINTICNATSLRQESVKKLAPEVDVMIIIGGKNSGNTRRLYYISKELNPNTYH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEECCCCCEEE
IETAEELQPEWFRGVKRVGISAGASTPDWIIEQVKSRIQEICEGQLVSS
ECCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVDIIIAEHAGFCFGVKRAVKLAEESLKESQGKVYTLGPIIHNPQEVNRLKNLGVFPSQG
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCCC
EEFKEGDTVIIRSHGIPPEKEEALRKKGLKVIDATCPYVKAVHEAVCQLTREGYFVVLVG
CCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEEE
EKNHPEVIGTLGYLRACNGKGIVVETLEDIGEALKHERVGIVAQTTQNEEFFKEVVGEIA
CCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
LWVKEVKVINTICNATSLRQESVKKLAPEVDVMIIIGGKNSGNTRRLYYISKELNPNTYH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEECCCCCEEE
IETAEELQPEWFRGVKRVGISAGASTPDWIIEQVKSRIQEICEGQLVSS
ECCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320; 12482608