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The map label for this gene is holB [H]

Identifier: 15606671

GI number: 15606671

Start: 1082598

End: 1083515

Strand: Direct

Name: holB [H]

Synonym: aq_1526

Alternate gene names: 15606671

Gene position: 1082598-1083515 (Clockwise)

Preceding gene: 15606670

Following gene: 15606672

Centisome position: 69.78

GC content: 41.83

Gene sequence:

>918_bases
ATGGAAAAAGTTTTTTTGGAAAAACTCCAGAAAACCTTGCACATACCCGGAGGACTCCTTTTTTACGGCAAAGAAGGAAG
CGGAAAGACGAAAACAGCTTTTGAATTTGCAAAAGGTATTTTATGTAAGGAAAACGTACCGTGGGGATGCGGAAGTTGTC
CCTCCTGCAAACACGTAAACGAGCTGGAGGAAGCCTTCTTTAAAGGAGAAATAGAAGACTTTAAAGTTTATAAAGACAAG
GACGGTAAAAAGCACTTCGTTTACCTTATGGGCGAACATCCCGACTTTGTGGTAATAATCCCGAGCGGACATTACATAAA
GATAGAACAGATAAGGGAAGTTAAGAACTTTGCCTATGTGAAGCCCGCACTAAGCAGGAGAAAAGTAATTATAATAGACG
ACGCCCACGCGATGACCTCTCAGGCGGCAAACGCTCTTTTAAAGGTATTGGAAGAGCCACCTGCGGACACCACCTTTATC
TTGACCACGAACAGGCGTTCTGCAATCCTGCCGACTATCCTCTCCAGAACTTTTCAAGTGGAGTTCAAGGGCTTTTCAGT
AAAAGAGGTTATGGAAATAGCGAAAGTAGACGAGGAAATAGCGAAACTCTCTGGAGGCAGTCTAAAAAGGGCTATCTTAC
TAAAGGAAAACAAAGATATCCTAAACAAAGTAAAGGAATTCTTGGAAAACGAGCCGTTAAAAGTTTACAAGCTTGCAAGT
GAATTCGAAAAGTGGGAACCTGAAAAGCAAAAACTCTTCCTTGAAATTATGGAAGAATTGGTATCTCAAAAATTGACCGA
AGAGAAAAAAGACAATTACACCTACCTTCTTGATACGATCAGACTCTTTAAAGACGGACTCGCAAGGGGTGTAAACGAAC
CTCTGTGGCTGTTTACGTTAGCCGTTCAGGCGGATTAA

Upstream 100 bases:

>100_bases
AACCGCTACTAAAAATTGATAAAAAACCGAAGAAAGAACTGAACCTATAGTCCGCGAAGTTGCAAAGGTTATAAACGAAA
CCTGTTTAAATTAAATTTGT

Downstream 100 bases:

>100_bases
TAAACCGTTATTGATTCCGTAACATTTAAACCTTAATCTAAATTATGAGAGCCTTTGAAGGAGGTCTGGTATGGAAAATT
TGAAGATTAGATATATAGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEKVFLEKLQKTLHIPGGLLFYGKEGSGKTKTAFEFAKGILCKENVPWGCGSCPSCKHVNELEEAFFKGEIEDFKVYKDK
DGKKHFVYLMGEHPDFVVIIPSGHYIKIEQIREVKNFAYVKPALSRRKVIIIDDAHAMTSQAANALLKVLEEPPADTTFI
LTTNRRSAILPTILSRTFQVEFKGFSVKEVMEIAKVDEEIAKLSGGSLKRAILLKENKDILNKVKEFLENEPLKVYKLAS
EFEKWEPEKQKLFLEIMEELVSQKLTEEKKDNYTYLLDTIRLFKDGLARGVNEPLWLFTLAVQAD

Sequences:

>Translated_305_residues
MEKVFLEKLQKTLHIPGGLLFYGKEGSGKTKTAFEFAKGILCKENVPWGCGSCPSCKHVNELEEAFFKGEIEDFKVYKDK
DGKKHFVYLMGEHPDFVVIIPSGHYIKIEQIREVKNFAYVKPALSRRKVIIIDDAHAMTSQAANALLKVLEEPPADTTFI
LTTNRRSAILPTILSRTFQVEFKGFSVKEVMEIAKVDEEIAKLSGGSLKRAILLKENKDILNKVKEFLENEPLKVYKLAS
EFEKWEPEKQKLFLEIMEELVSQKLTEEKKDNYTYLLDTIRLFKDGLARGVNEPLWLFTLAVQAD
>Mature_305_residues
MEKVFLEKLQKTLHIPGGLLFYGKEGSGKTKTAFEFAKGILCKENVPWGCGSCPSCKHVNELEEAFFKGEIEDFKVYKDK
DGKKHFVYLMGEHPDFVVIIPSGHYIKIEQIREVKNFAYVKPALSRRKVIIIDDAHAMTSQAANALLKVLEEPPADTTFI
LTTNRRSAILPTILSRTFQVEFKGFSVKEVMEIAKVDEEIAKLSGGSLKRAILLKENKDILNKVKEFLENEPLKVYKLAS
EFEKWEPEKQKLFLEIMEELVSQKLTEEKKDNYTYLLDTIRLFKDGLARGVNEPLWLFTLAVQAD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34897; Mature: 34897

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVFLEKLQKTLHIPGGLLFYGKEGSGKTKTAFEFAKGILCKENVPWGCGSCPSCKHVN
CCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCEECCCCCCCCCCCCCCCHHH
ELEEAFFKGEIEDFKVYKDKDGKKHFVYLMGEHPDFVVIIPSGHYIKIEQIREVKNFAYV
HHHHHHHCCCCHHEEEEECCCCCEEEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHH
KPALSRRKVIIIDDAHAMTSQAANALLKVLEEPPADTTFILTTNRRSAILPTILSRTFQV
CHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHEEEE
EFKGFSVKEVMEIAKVDEEIAKLSGGSLKRAILLKENKDILNKVKEFLENEPLKVYKLAS
EECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHH
EFEKWEPEKQKLFLEIMEELVSQKLTEEKKDNYTYLLDTIRLFKDGLARGVNEPLWLFTL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
AVQAD
EEECC
>Mature Secondary Structure
MEKVFLEKLQKTLHIPGGLLFYGKEGSGKTKTAFEFAKGILCKENVPWGCGSCPSCKHVN
CCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCEECCCCCCCCCCCCCCCHHH
ELEEAFFKGEIEDFKVYKDKDGKKHFVYLMGEHPDFVVIIPSGHYIKIEQIREVKNFAYV
HHHHHHHCCCCHHEEEEECCCCCEEEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHH
KPALSRRKVIIIDDAHAMTSQAANALLKVLEEPPADTTFILTTNRRSAILPTILSRTFQV
CHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHEEEE
EFKGFSVKEVMEIAKVDEEIAKLSGGSLKRAILLKENKDILNKVKEFLENEPLKVYKLAS
EECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHH
EFEKWEPEKQKLFLEIMEELVSQKLTEEKKDNYTYLLDTIRLFKDGLARGVNEPLWLFTL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
AVQAD
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]