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The map label for this gene is rsuA [C]

Identifier: 15606629

GI number: 15606629

Start: 1043006

End: 1043755

Strand: Direct

Name: rsuA [C]

Synonym: aq_1464

Alternate gene names: 15606629

Gene position: 1043006-1043755 (Clockwise)

Preceding gene: 15606628

Following gene: 15606630

Centisome position: 67.23

GC content: 46.8

Gene sequence:

>750_bases
ATGATGGAAGTGAGGATAAATAAGTTCTTATCGGAAGCGGGAGTAGCGTCGCGCAGAAAAGCGGAAAAACTAATTCTGGA
GGGCAGAGTAAAGGTAAACGGAGAAGTCGTAAGGAGCTTGGGAGTAAAAGTAAACCCAGAAGTGGACATAGTTGAAGTGG
ACGGAAAACCCGTAAAGCCCCAGAGAAAACGGTACATAATCCTGAACAAGCCCTGTTGCTATTTGACTCAGCTCGGAAGG
TCCCCTGACGGGAGGAAGACTATTGAAGAGCTTATAAAGGACATACCCGAGAGGGTATTTCCTGTGGGAAGGCTTGATTA
CAACACTGAAGGGCTTTTGATACTAACCAACGACGGAGAGCTCGCAAACAGGATACTCCACCCGAGGTACAAACTGCCGA
AGGTTTACCTCGCACTCGTTGAAGGGAAAGTAGACCAGAAAACTTTAAAGAGGATGAAGCAGGGAATAGAACTGGAGGAT
GGCTTTGCAAAACCCGACAACATAAGGATAGTACGCTACGAAGGGAAAAACACACTCCTTGAGATAACATTCCACGAAGG
GAGGAAGCACTTAGTTAAGAGGTTTTTAGGAGCTTTCGGACATAAGGTAAAGCGTTTAAAGAGGATAGCTATCGGACCAA
TAAAACTCGGTAAGCTCTCTCCGGGCAAGTGGAGGGAACTTAATCAGGGGGAACTTGCCCAGCTCTTTAAAGCGGTGGGA
CTGAAGTACGTTCCGAGGAAGAGGAGATGA

Upstream 100 bases:

>100_bases
TCATAGGCCCCCACATTTCCCACATTCTGGGCGAAAGGAGGCTCAGACTTTTACTCGGCTTCATACTCCTCGGTATAGGA
CTTAAGTATATCTTTATAAG

Downstream 100 bases:

>100_bases
TAAACAGGAAAGTTGTTTACGCACTTTCCGCCCTTTTGCTCTTCGTGTATTCCTATGCCTTTATAAGCGACTTTTCGGAA
TTTAAAAACTTCCTGAACAT

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MMEVRINKFLSEAGVASRRKAEKLILEGRVKVNGEVVRSLGVKVNPEVDIVEVDGKPVKPQRKRYIILNKPCCYLTQLGR
SPDGRKTIEELIKDIPERVFPVGRLDYNTEGLLILTNDGELANRILHPRYKLPKVYLALVEGKVDQKTLKRMKQGIELED
GFAKPDNIRIVRYEGKNTLLEITFHEGRKHLVKRFLGAFGHKVKRLKRIAIGPIKLGKLSPGKWRELNQGELAQLFKAVG
LKYVPRKRR

Sequences:

>Translated_249_residues
MMEVRINKFLSEAGVASRRKAEKLILEGRVKVNGEVVRSLGVKVNPEVDIVEVDGKPVKPQRKRYIILNKPCCYLTQLGR
SPDGRKTIEELIKDIPERVFPVGRLDYNTEGLLILTNDGELANRILHPRYKLPKVYLALVEGKVDQKTLKRMKQGIELED
GFAKPDNIRIVRYEGKNTLLEITFHEGRKHLVKRFLGAFGHKVKRLKRIAIGPIKLGKLSPGKWRELNQGELAQLFKAVG
LKYVPRKRR
>Mature_249_residues
MMEVRINKFLSEAGVASRRKAEKLILEGRVKVNGEVVRSLGVKVNPEVDIVEVDGKPVKPQRKRYIILNKPCCYLTQLGR
SPDGRKTIEELIKDIPERVFPVGRLDYNTEGLLILTNDGELANRILHPRYKLPKVYLALVEGKVDQKTLKRMKQGIELED
GFAKPDNIRIVRYEGKNTLLEITFHEGRKHLVKRFLGAFGHKVKRLKRIAIGPIKLGKLSPGKWRELNQGELAQLFKAVG
LKYVPRKRR

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=35.1694915254237, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=38.1526104417671, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=33.195020746888, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=34.9726775956284, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1464_AQUAE (O67444)

Other databases:

- EMBL:   AE000657
- PIR:   B70427
- RefSeq:   NP_214009.1
- ProteinModelPortal:   O67444
- SMR:   O67444
- GeneID:   1192975
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1464
- NMPDR:   fig|224324.1.peg.1024
- HOGENOM:   HBG754478
- OMA:   LPYRVFT
- PhylomeDB:   O67444
- ProtClustDB:   CLSK2299709
- BioCyc:   AAEO224324:AQ_1464-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28518; Mature: 28518

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEVRINKFLSEAGVASRRKAEKLILEGRVKVNGEVVRSLGVKVNPEVDIVEVDGKPVKP
CCEEHHHHHHHHHCHHHHHHHHHHHHHCCEEECHHHHHHCCCEECCCEEEEECCCCCCCC
QRKRYIILNKPCCYLTQLGRSPDGRKTIEELIKDIPERVFPVGRLDYNTEGLLILTNDGE
CCCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
LANRILHPRYKLPKVYLALVEGKVDQKTLKRMKQGIELEDGFAKPDNIRIVRYEGKNTLL
HHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEE
EITFHEGRKHLVKRFLGAFGHKVKRLKRIAIGPIKLGKLSPGKWRELNQGELAQLFKAVG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
LKYVPRKRR
CCCCCCCCC
>Mature Secondary Structure
MMEVRINKFLSEAGVASRRKAEKLILEGRVKVNGEVVRSLGVKVNPEVDIVEVDGKPVKP
CCEEHHHHHHHHHCHHHHHHHHHHHHHCCEEECHHHHHHCCCEECCCEEEEECCCCCCCC
QRKRYIILNKPCCYLTQLGRSPDGRKTIEELIKDIPERVFPVGRLDYNTEGLLILTNDGE
CCCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
LANRILHPRYKLPKVYLALVEGKVDQKTLKRMKQGIELEDGFAKPDNIRIVRYEGKNTLL
HHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEE
EITFHEGRKHLVKRFLGAFGHKVKRLKRIAIGPIKLGKLSPGKWRELNQGELAQLFKAVG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
LKYVPRKRR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320