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The map label for this gene is nuoI2

Identifier: 15606570

GI number: 15606570

Start: 978259

End: 978885

Strand: Direct

Name: nuoI2

Synonym: aq_1375

Alternate gene names: 15606570

Gene position: 978259-978885 (Clockwise)

Preceding gene: 15606569

Following gene: 15606571

Centisome position: 63.06

GC content: 46.41

Gene sequence:

>627_bases
ATGATTAAGAAGGTAGCTGCAAAGCCTCTCAGCTGGCTTGAGAGGATATTTTTCATAGATTTCATAAAAGGTCTCAGGAT
TACATTAAAGAACGCCCTGAGGAAAACTATCACCACTCACTACCCTTACGAAAAAATAACTCCGCCGAAACGCTTCAGGG
GTTACTTTGCACACAAGGTGGTTGACGGAACGGAACCCCAGCCGGCGTTTCAGGAATGGGTAAACAGGTACAACATCTTG
GTTGAGTATGGAAAGAGCCGGTGTGTCGTGTGCCTCAGGTGTAAAAGGGCTTGTCCTGTTCCTCAGCTCTTTGAAATAGA
AGGGAAAAAACTCCCAAACGGCAAAAGAGTTGTTAGCGTCTTTAACATGAACATGCTCCTCTGCACTTATTGCGGTTTTT
GCGTGGACGCATGCCCTGTGGATTGCCTTTATCAAACAGACATACACGAAAATGCCTCTTACACCAGGAAGGACGCTGTA
CTCACCCTTGAAATCCTTGAACAGATAGGCAGGGACTGGCAGAGAAGAAGGGAAAGAGAGCCCGACAGGATATGGATAGA
CGACGAGCAAAGGATGAAGCTGTGGGGTGAGAATAATGTAAAATTACCTAAACCCGAGGAGGTGTGA

Upstream 100 bases:

>100_bases
CCAGATTACTCAAAACGCATGGAAAATAATGCTCCCTCTTACACTCTTCAACATTGTTCTCACAGCTGTGTTAGCTCCAA
TAGTTTGGAGGTGATCGGCC

Downstream 100 bases:

>100_bases
TGGGGAGACTGCTTATATTTGGATTCTTCTCTATTCTTGCTATTTTGTCCGGTATAGGGATAATCACGCTAAGAAATCCG
GTTTACGTTGTAGTAGCCCT

Product: NADH dehydrogenase I chain I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 2; NDH-1 subunit I 2

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIKKVAAKPLSWLERIFFIDFIKGLRITLKNALRKTITTHYPYEKITPPKRFRGYFAHKVVDGTEPQPAFQEWVNRYNIL
VEYGKSRCVVCLRCKRACPVPQLFEIEGKKLPNGKRVVSVFNMNMLLCTYCGFCVDACPVDCLYQTDIHENASYTRKDAV
LTLEILEQIGRDWQRRREREPDRIWIDDEQRMKLWGENNVKLPKPEEV

Sequences:

>Translated_208_residues
MIKKVAAKPLSWLERIFFIDFIKGLRITLKNALRKTITTHYPYEKITPPKRFRGYFAHKVVDGTEPQPAFQEWVNRYNIL
VEYGKSRCVVCLRCKRACPVPQLFEIEGKKLPNGKRVVSVFNMNMLLCTYCGFCVDACPVDCLYQTDIHENASYTRKDAV
LTLEILEQIGRDWQRRREREPDRIWIDDEQRMKLWGENNVKLPKPEEV
>Mature_208_residues
MIKKVAAKPLSWLERIFFIDFIKGLRITLKNALRKTITTHYPYEKITPPKRFRGYFAHKVVDGTEPQPAFQEWVNRYNIL
VEYGKSRCVVCLRCKRACPVPQLFEIEGKKLPNGKRVVSVFNMNMLLCTYCGFCVDACPVDCLYQTDIHENASYTRKDAV
LTLEILEQIGRDWQRRREREPDRIWIDDEQRMKLWGENNVKLPKPEEV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=169, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17555194, Length=156, Percent_Identity=30.1282051282051, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864306, Length=159, Percent_Identity=29.559748427673, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI2_AQUAE (O67386)

Other databases:

- EMBL:   AE000657
- PIR:   G70419
- RefSeq:   NP_213950.1
- ProteinModelPortal:   O67386
- SMR:   O67386
- GeneID:   1192916
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1375
- NMPDR:   fig|224324.1.peg.965
- HOGENOM:   HBG724359
- OMA:   DIHENAS
- ProtClustDB:   CLSK2459820
- BioCyc:   AAEO224324:AQ_1375-MONOMER
- BRENDA:   1.6.99.5
- HAMAP:   MF_01351
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10

Pfam domain/function: NA

EC number: =1.6.99.5

Molecular weight: Translated: 24572; Mature: 24572

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKVAAKPLSWLERIFFIDFIKGLRITLKNALRKTITTHYPYEKITPPKRFRGYFAHKV
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHH
VDGTEPQPAFQEWVNRYNILVEYGKSRCVVCLRCKRACPVPQLFEIEGKKLPNGKRVVSV
CCCCCCCHHHHHHHHHHHHHHEECCCCEEEEEEHHCCCCCCCEEECCCCCCCCCHHEEHH
FNMNMLLCTYCGFCVDACPVDCLYQTDIHENASYTRKDAVLTLEILEQIGRDWQRRRERE
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDRIWIDDEQRMKLWGENNVKLPKPEEV
CCEEEECCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKVAAKPLSWLERIFFIDFIKGLRITLKNALRKTITTHYPYEKITPPKRFRGYFAHKV
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHH
VDGTEPQPAFQEWVNRYNILVEYGKSRCVVCLRCKRACPVPQLFEIEGKKLPNGKRVVSV
CCCCCCCHHHHHHHHHHHHHHEECCCCEEEEEEHHCCCCCCCEEECCCCCCCCCHHEEHH
FNMNMLLCTYCGFCVDACPVDCLYQTDIHENASYTRKDAVLTLEILEQIGRDWQRRRERE
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDRIWIDDEQRMKLWGENNVKLPKPEEV
CCEEEECCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320