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The map label for this gene is moeB [H]

Identifier: 15606532

GI number: 15606532

Start: 942990

End: 943805

Strand: Direct

Name: moeB [H]

Synonym: aq_1329

Alternate gene names: 15606532

Gene position: 942990-943805 (Clockwise)

Preceding gene: 15606530

Following gene: 15606533

Centisome position: 60.79

GC content: 47.43

Gene sequence:

>816_bases
ATGTTTAACTTTACCGAGGAGCAAATTAAGAGGTACGCCCGTCACATAATCCTCCCAGAAGTCGGAGGAAAGGGACAGGC
TAAACTCCTCCAATCTAAAGTGCTCGTTATAGGAGCCGGGGGACTGGGTTCTCCCGCACTATACTACCTCGCCGCTGCGG
GTGTTGGAACTATAGGAATAGTTGACTTTGACGTTGTAGACTTTTCAAACCTACAGAGGCAGATACTCCACACGACAGAA
AGGGTCGGAACTCCCAAGGTGGAGAGTGCAAAAAAGACTTTGGAAGCCCTTAACCCAGACGTTAAAGTTATTACGTACAA
CACAAAGATAAATAAAAACAACGTAATGGACATTATTAAGGACTACGACGTCATTCTCGACGGGACAGATAACTTCCCCA
CGAGGTTTTTAATAAACGACGCCTGTTACTTTGCAGGGAAACCCCTTGTTTCCGCTGCCATGCTGAGATTTGAGGGGCAG
TGCACCGTTTTTGACTTTAGGGACAAGGAAAACTCTCCCTGTTACAGGTGCTTATTCCCCGAACCGCCACCGCCTGGGCT
CGTGCCCTCCTGTCAGGAAGCGGGAATTCTCGGTTCTGTTGGCGGAATTATGGGAAGCATACAGGCAACCGAAGCGATAA
AGCTCCTCCTCGGAATAGGCGAACCCTTAGTGGGTAAGCTCCTCATAATGGATGCTCTATCTATGGACTTTAGAAAGGTA
AAACTCAGGAAAGATCCAAAGTGTCCCCTTTGCGGAGAAAATCCAAAGATAAAGGAACTCATAGAGTACGACCAGAGCTG
TGAAGTGAGGTTTTAA

Upstream 100 bases:

>100_bases
TCTTGAACTCGGATTTCTCAAGAACGATGAAGTGTTTCATTCTAAATTAATATTAACCCTGACATGGAAACTCTCTTCCT
TTTGTCTATAATTTATATGC

Downstream 100 bases:

>100_bases
GTGAAAAAACTCCTTTCCCTTGAGAACCTCACGCTCCTTTCCCTAATCCTTGCCGTCTTTTTTGGTATTTACTTTAAAGA
AATAGCCCTGAATATCAAAC

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MFNFTEEQIKRYARHIILPEVGGKGQAKLLQSKVLVIGAGGLGSPALYYLAAAGVGTIGIVDFDVVDFSNLQRQILHTTE
RVGTPKVESAKKTLEALNPDVKVITYNTKINKNNVMDIIKDYDVILDGTDNFPTRFLINDACYFAGKPLVSAAMLRFEGQ
CTVFDFRDKENSPCYRCLFPEPPPPGLVPSCQEAGILGSVGGIMGSIQATEAIKLLLGIGEPLVGKLLIMDALSMDFRKV
KLRKDPKCPLCGENPKIKELIEYDQSCEVRF

Sequences:

>Translated_271_residues
MFNFTEEQIKRYARHIILPEVGGKGQAKLLQSKVLVIGAGGLGSPALYYLAAAGVGTIGIVDFDVVDFSNLQRQILHTTE
RVGTPKVESAKKTLEALNPDVKVITYNTKINKNNVMDIIKDYDVILDGTDNFPTRFLINDACYFAGKPLVSAAMLRFEGQ
CTVFDFRDKENSPCYRCLFPEPPPPGLVPSCQEAGILGSVGGIMGSIQATEAIKLLLGIGEPLVGKLLIMDALSMDFRKV
KLRKDPKCPLCGENPKIKELIEYDQSCEVRF
>Mature_271_residues
MFNFTEEQIKRYARHIILPEVGGKGQAKLLQSKVLVIGAGGLGSPALYYLAAAGVGTIGIVDFDVVDFSNLQRQILHTTE
RVGTPKVESAKKTLEALNPDVKVITYNTKINKNNVMDIIKDYDVILDGTDNFPTRFLINDACYFAGKPLVSAAMLRFEGQ
CTVFDFRDKENSPCYRCLFPEPPPPGLVPSCQEAGILGSVGGIMGSIQATEAIKLLLGIGEPLVGKLLIMDALSMDFRKV
KLRKDPKCPLCGENPKIKELIEYDQSCEVRF

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=265, Percent_Identity=44.5283018867925, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI4885649, Length=151, Percent_Identity=31.1258278145695, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI38045942, Length=107, Percent_Identity=39.2523364485981, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI38045944, Length=107, Percent_Identity=39.2523364485981, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI38045948, Length=152, Percent_Identity=32.2368421052632, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787048, Length=253, Percent_Identity=43.8735177865613, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=43.3734939759036, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17540406, Length=261, Percent_Identity=41.7624521072797, Blast_Score=236, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI193203301, Length=167, Percent_Identity=32.3353293413174, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71984476, Length=118, Percent_Identity=35.5932203389831, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=262, Percent_Identity=48.0916030534351, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6320598, Length=146, Percent_Identity=32.8767123287671, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322639, Length=193, Percent_Identity=31.0880829015544, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582879, Length=265, Percent_Identity=49.0566037735849, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24660640, Length=153, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28573937, Length=161, Percent_Identity=31.6770186335404, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24641311, Length=220, Percent_Identity=27.7272727272727, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20129973, Length=138, Percent_Identity=31.8840579710145, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 29776; Mature: 29776

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNFTEEQIKRYARHIILPEVGGKGQAKLLQSKVLVIGAGGLGSPALYYLAAAGVGTIGI
CCCCCHHHHHHHHHHEECCCCCCCCHHHHHHCEEEEEECCCCCCHHHHHHHHCCCCEEEE
VDFDVVDFSNLQRQILHTTERVGTPKVESAKKTLEALNPDVKVITYNTKINKNNVMDIIK
EEEEHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECEECCCHHHHHHH
DYDVILDGTDNFPTRFLINDACYFAGKPLVSAAMLRFEGQCTVFDFRDKENSPCYRCLFP
CCEEEEECCCCCCCEEEEECHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECC
EPPPPGLVPSCQEAGILGSVGGIMGSIQATEAIKLLLGIGEPLVGKLLIMDALSMDFRKV
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
KLRKDPKCPLCGENPKIKELIEYDQSCEVRF
HCCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFNFTEEQIKRYARHIILPEVGGKGQAKLLQSKVLVIGAGGLGSPALYYLAAAGVGTIGI
CCCCCHHHHHHHHHHEECCCCCCCCHHHHHHCEEEEEECCCCCCHHHHHHHHCCCCEEEE
VDFDVVDFSNLQRQILHTTERVGTPKVESAKKTLEALNPDVKVITYNTKINKNNVMDIIK
EEEEHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECEECCCHHHHHHH
DYDVILDGTDNFPTRFLINDACYFAGKPLVSAAMLRFEGQCTVFDFRDKENSPCYRCLFP
CCEEEEECCCCCCCEEEEECHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECC
EPPPPGLVPSCQEAGILGSVGGIMGSIQATEAIKLLLGIGEPLVGKLLIMDALSMDFRKV
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
KLRKDPKCPLCGENPKIKELIEYDQSCEVRF
HCCCCCCCCCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]