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The map label for this gene is tgt

Identifier: 15606515

GI number: 15606515

Start: 926031

End: 927167

Strand: Direct

Name: tgt

Synonym: aq_1308

Alternate gene names: 15606515

Gene position: 926031-927167 (Clockwise)

Preceding gene: 15606514

Following gene: 15606517

Centisome position: 59.69

GC content: 43.54

Gene sequence:

>1137_bases
ATGTTTAAATTCCACCTGATTACTAAAGACGGAAAAGCGAGAAGAGGTAGGATATACACCCCTCACGGAGTTATAGAAAC
CCCTGTTTTTATGCCCGTGGGAACTCAGGGAACAGTAAAGGCGATGCTCCACAAGCTCCTTGATGAGATAGGCACTCAAA
TAATCCTCGGAAACACCTACCACCTTTATCTGAGACCGGGAACAGAAATAATAAAGAAGGCCGGAGGACTGCACAGATTT
ATATCCTGGAATAAACCTATATTGACCGACAGCGGAGGTTATCAGGTATTCTCCTTGGCAAAAGGGAAGTTCGGAAACAG
AAAAGCAAAGGTAAAGGTGAGTGATGAAGGTGTTGAATTTCAGGATCACCTTCAGGGAGATAAACATTTCTTTACTCCGG
AAAAAGTTGTGGAAATTCAGGAAATTTTCGGCTCCGACATAATGATGCCCCTTGACGAGTGCGTGGAATACCCTGTGGAT
AAAAACTACGCGGAAAAGGCTTTAAAAAGGACGATAAACTGGCTTGAAAGGAGCATAAAGGCAAAAAAGAGGGAGGATCA
GGTTTTATTCGGAATAGTTCAGGGAGCCTTCTGGAAAGACTTGAGGAAAAAGGCTGTTGAAGAGACCTTAAAGTTTGACG
AATTTCTCTTCGGATACTCTATCGGCGGGCTTTCTGTCGGAGAACCAAAGGAGATTATGTACGGAATGACGGAAGTTGTA
TGCGAGCTTCTTCCCGAAAAGAAACCCAGGTACTTAATGGGGGTGGGGAAGCCAGAAGATATACTCGAAGCAGTGGAAAG
AGGAGTGGATATGTTTGACTGTGTAGTTCCCACAAGAAATGCAAGAACCGGAACGCTTTACACGTCTCAGGGAGTTGTTG
ACATAAGGCACTCCAAATGGAAAGAAGATTTCTCACCCCTTGATCCCGAGTGCGATTGTTACACCTGCAGAAATTTCTCA
AAGGCTTACCTCAGACACCTCTTCGTTGCAGAAGAAATATCCGCCTACGTTTTAAATACCATTCATAACTTGAGGTTTTA
CCTGAAAATGATGGAGGAAGTGAGGAAGGCGATAGAGGAAAAGAGGTTCAAGGAGTTGAAGGAAAAGTACCTTCAAAGGG
TTAAGAATAAACTTTGA

Upstream 100 bases:

>100_bases
AGAAGAAGAACAAATTAAGGAAGGACATACATTTACGGACGAGCAAGCTTCTGACCTCGGAATGTAATTCTCCTTCCCTC
CCCTTTTAAAATCTTCTTTT

Downstream 100 bases:

>100_bases
CTCCTTCGTTTCTCGCTTTTAGAACCATTACCTGTGCATCGTATCCGGAAAACTTGGTTATTACTTCTCTTATAGCTTCT
CCTATTTTTTCTTCATTCTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MFKFHLITKDGKARRGRIYTPHGVIETPVFMPVGTQGTVKAMLHKLLDEIGTQIILGNTYHLYLRPGTEIIKKAGGLHRF
ISWNKPILTDSGGYQVFSLAKGKFGNRKAKVKVSDEGVEFQDHLQGDKHFFTPEKVVEIQEIFGSDIMMPLDECVEYPVD
KNYAEKALKRTINWLERSIKAKKREDQVLFGIVQGAFWKDLRKKAVEETLKFDEFLFGYSIGGLSVGEPKEIMYGMTEVV
CELLPEKKPRYLMGVGKPEDILEAVERGVDMFDCVVPTRNARTGTLYTSQGVVDIRHSKWKEDFSPLDPECDCYTCRNFS
KAYLRHLFVAEEISAYVLNTIHNLRFYLKMMEEVRKAIEEKRFKELKEKYLQRVKNKL

Sequences:

>Translated_378_residues
MFKFHLITKDGKARRGRIYTPHGVIETPVFMPVGTQGTVKAMLHKLLDEIGTQIILGNTYHLYLRPGTEIIKKAGGLHRF
ISWNKPILTDSGGYQVFSLAKGKFGNRKAKVKVSDEGVEFQDHLQGDKHFFTPEKVVEIQEIFGSDIMMPLDECVEYPVD
KNYAEKALKRTINWLERSIKAKKREDQVLFGIVQGAFWKDLRKKAVEETLKFDEFLFGYSIGGLSVGEPKEIMYGMTEVV
CELLPEKKPRYLMGVGKPEDILEAVERGVDMFDCVVPTRNARTGTLYTSQGVVDIRHSKWKEDFSPLDPECDCYTCRNFS
KAYLRHLFVAEEISAYVLNTIHNLRFYLKMMEEVRKAIEEKRFKELKEKYLQRVKNKL
>Mature_378_residues
MFKFHLITKDGKARRGRIYTPHGVIETPVFMPVGTQGTVKAMLHKLLDEIGTQIILGNTYHLYLRPGTEIIKKAGGLHRF
ISWNKPILTDSGGYQVFSLAKGKFGNRKAKVKVSDEGVEFQDHLQGDKHFFTPEKVVEIQEIFGSDIMMPLDECVEYPVD
KNYAEKALKRTINWLERSIKAKKREDQVLFGIVQGAFWKDLRKKAVEETLKFDEFLFGYSIGGLSVGEPKEIMYGMTEVV
CELLPEKKPRYLMGVGKPEDILEAVERGVDMFDCVVPTRNARTGTLYTSQGVVDIRHSKWKEDFSPLDPECDCYTCRNFS
KAYLRHLFVAEEISAYVLNTIHNLRFYLKMMEEVRKAIEEKRFKELKEKYLQRVKNKL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=44.5983379501385, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI13375872, Length=251, Percent_Identity=26.2948207171315, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786607, Length=377, Percent_Identity=52.2546419098143, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=40.7008086253369, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI25146502, Length=237, Percent_Identity=28.2700421940928, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920510, Length=364, Percent_Identity=42.5824175824176, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI21355311, Length=258, Percent_Identity=24.4186046511628, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_AQUAE (O67331)

Other databases:

- EMBL:   AE000657
- PIR:   H70412
- RefSeq:   NP_213895.1
- ProteinModelPortal:   O67331
- SMR:   O67331
- GeneID:   1192769
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1308
- NMPDR:   fig|224324.1.peg.910
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   O67331
- ProtClustDB:   PRK00112
- BioCyc:   AAEO224324:AQ_1308-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43654; Mature: 43654

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: ACT_SITE 90-90 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFHLITKDGKARRGRIYTPHGVIETPVFMPVGTQGTVKAMLHKLLDEIGTQIILGNTY
CEEEEEEECCCCCCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEECCEE
HLYLRPGTEIIKKAGGLHRFISWNKPILTDSGGYQVFSLAKGKFGNRKAKVKVSDEGVEF
EEEECCCHHHHHHCCCHHHHHCCCCCEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCH
QDHLQGDKHFFTPEKVVEIQEIFGSDIMMPLDECVEYPVDKNYAEKALKRTINWLERSIK
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AKKREDQVLFGIVQGAFWKDLRKKAVEETLKFDEFLFGYSIGGLSVGEPKEIMYGMTEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
CELLPEKKPRYLMGVGKPEDILEAVERGVDMFDCVVPTRNARTGTLYTSQGVVDIRHSKW
HHHCCCCCCCEEEECCCHHHHHHHHHCCCCCEEHCCCCCCCCCCEEEECCCEEECHHHHH
KEDFSPLDPECDCYTCRNFSKAYLRHLFVAEEISAYVLNTIHNLRFYLKMMEEVRKAIEE
HHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRFKELKEKYLQRVKNKL
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKFHLITKDGKARRGRIYTPHGVIETPVFMPVGTQGTVKAMLHKLLDEIGTQIILGNTY
CEEEEEEECCCCCCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEECCEE
HLYLRPGTEIIKKAGGLHRFISWNKPILTDSGGYQVFSLAKGKFGNRKAKVKVSDEGVEF
EEEECCCHHHHHHCCCHHHHHCCCCCEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCH
QDHLQGDKHFFTPEKVVEIQEIFGSDIMMPLDECVEYPVDKNYAEKALKRTINWLERSIK
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AKKREDQVLFGIVQGAFWKDLRKKAVEETLKFDEFLFGYSIGGLSVGEPKEIMYGMTEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
CELLPEKKPRYLMGVGKPEDILEAVERGVDMFDCVVPTRNARTGTLYTSQGVVDIRHSKW
HHHCCCCCCCEEEECCCHHHHHHHHHCCCCCEEHCCCCCCCCCCEEEECCCEEECHHHHH
KEDFSPLDPECDCYTCRNFSKAYLRHLFVAEEISAYVLNTIHNLRFYLKMMEEVRKAIEE
HHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRFKELKEKYLQRVKNKL
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320