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The map label for this gene is metK

Identifier: 15606406

GI number: 15606406

Start: 809561

End: 810691

Strand: Direct

Name: metK

Synonym: aq_1154

Alternate gene names: 15606406

Gene position: 809561-810691 (Clockwise)

Preceding gene: 15606400

Following gene: 15606410

Centisome position: 52.18

GC content: 48.63

Gene sequence:

>1131_bases
ATGTACAACCTCAGAATGGCTGAATCCGTAACGGAAGGCCACCCGGACAAAATAGCGGATCAACTCGCTGATGCACTCCT
TGACGAGTTCATAAAGAAGGATCCTTACAGCAAAGTCTCCTTAGAGATTATGGTAACCACAGGACTCGTTATGGTAGGAG
GGGAGCTCACCACGGAAAGCTACGTGGACATCCCAAGAGTTGTGAGAAGCGTTATTAAGGATATCGGATACACGAGACCC
GAACTCGGTTTTGATGCGGACACCTGCGCTGTAGTTCAGTCCATAGACGAGCAGAGTCCCGAAATTGCTTTAGGTATTTC
CTCGGAGGGTGCGGGAGACACCGCGATAGTTGTGGGCTACGCCACGAAGGAAGCTCCTAACCTCATGCCCTGGCCCATAA
CAATAGCCCACAAGATCACAAAGAGGATATCCGAGTACAGGAAGATAGGAAAGTTTCCCTTCCTGAGGCCAGACGGAAAG
GTTCTCGTGGCTATGATTTACGAGGACGGGAAGCCCTCTTACGTTCAGAGCATAGTTGCCTACGTCCACCACGACCCCGA
CGTTTCCATAAATCACCTGAGGGAACTAATAATTGAAGAAATTATTAAAAAGGAAATTCCTGAGGAGTTCTTGACGGAAA
AAACGAGCATAAAGGTAAACCCCACAGGCAGGTTCGTAATTGGTGGACCCGTTGCGGACACGGGACTCACAGGAAGGAAG
ATAGTTTCCGACGCATACGGAGATATAGGACTCTCCGGCGGTAGTGCCTTTTCAGGCAAGGACCCGACGAAGACAGATCG
TTCAGGTTCTTACCTTGCGAGGATGATAGCGAAACACGTTGTTGCCGGTGGATGGGCAGAGAGGTGTTTGGTTCAGATAG
GGTACGCCTTCGGACTTACCGAACCCGTTGCTTTTGACATAGAAACCTTCGGAACGGAGAAGATAAGCAAAGAAATACTG
GAAGACGCTGTAAAGAAGGTATTTCCATTAAGACCAGCGGAAATAATAGAGTTCCTTGACCTCAGGAAACCCATATACAG
GCAAACATCCGTTTACGGACACTTCGGAAAGGAAAACCTCCCATGGGAAAAGTTAACGAAGCTTGAGGAGCTGAAGGAGT
TACTCGATTAA

Upstream 100 bases:

>100_bases
CAAAGTTCTATCCTCTCTAGGGTTCCCACGGAGGTTCCGCCGAACTTCTGAACGAGTATTTTCATCGGAAAGTAATATTT
TACCTTAAAATTTATTAATT

Downstream 100 bases:

>100_bases
ATCAAGAAATTCTCTTATTTTCATTACCACGTAATCCTTCGGAGTTGTGTAAACCTTAACTATTTCATCTTCCACGAGGA
GTACTTCCTTAAAACCCTTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYNLRMAESVTEGHPDKIADQLADALLDEFIKKDPYSKVSLEIMVTTGLVMVGGELTTESYVDIPRVVRSVIKDIGYTRP
ELGFDADTCAVVQSIDEQSPEIALGISSEGAGDTAIVVGYATKEAPNLMPWPITIAHKITKRISEYRKIGKFPFLRPDGK
VLVAMIYEDGKPSYVQSIVAYVHHDPDVSINHLRELIIEEIIKKEIPEEFLTEKTSIKVNPTGRFVIGGPVADTGLTGRK
IVSDAYGDIGLSGGSAFSGKDPTKTDRSGSYLARMIAKHVVAGGWAERCLVQIGYAFGLTEPVAFDIETFGTEKISKEIL
EDAVKKVFPLRPAEIIEFLDLRKPIYRQTSVYGHFGKENLPWEKLTKLEELKELLD

Sequences:

>Translated_376_residues
MYNLRMAESVTEGHPDKIADQLADALLDEFIKKDPYSKVSLEIMVTTGLVMVGGELTTESYVDIPRVVRSVIKDIGYTRP
ELGFDADTCAVVQSIDEQSPEIALGISSEGAGDTAIVVGYATKEAPNLMPWPITIAHKITKRISEYRKIGKFPFLRPDGK
VLVAMIYEDGKPSYVQSIVAYVHHDPDVSINHLRELIIEEIIKKEIPEEFLTEKTSIKVNPTGRFVIGGPVADTGLTGRK
IVSDAYGDIGLSGGSAFSGKDPTKTDRSGSYLARMIAKHVVAGGWAERCLVQIGYAFGLTEPVAFDIETFGTEKISKEIL
EDAVKKVFPLRPAEIIEFLDLRKPIYRQTSVYGHFGKENLPWEKLTKLEELKELLD
>Mature_376_residues
MYNLRMAESVTEGHPDKIADQLADALLDEFIKKDPYSKVSLEIMVTTGLVMVGGELTTESYVDIPRVVRSVIKDIGYTRP
ELGFDADTCAVVQSIDEQSPEIALGISSEGAGDTAIVVGYATKEAPNLMPWPITIAHKITKRISEYRKIGKFPFLRPDGK
VLVAMIYEDGKPSYVQSIVAYVHHDPDVSINHLRELIIEEIIKKEIPEEFLTEKTSIKVNPTGRFVIGGPVADTGLTGRK
IVSDAYGDIGLSGGSAFSGKDPTKTDRSGSYLARMIAKHVVAGGWAERCLVQIGYAFGLTEPVAFDIETFGTEKISKEIL
EDAVKKVFPLRPAEIIEFLDLRKPIYRQTSVYGHFGKENLPWEKLTKLEELKELLD

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=376, Percent_Identity=45.4787234042553, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=45.6989247311828, Blast_Score=332, Evalue=3e-91,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=49.2021276595745, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538498, Length=382, Percent_Identity=45.0261780104712, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17551082, Length=383, Percent_Identity=44.3864229765013, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17538494, Length=382, Percent_Identity=45.0261780104712, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25145633, Length=381, Percent_Identity=44.6194225721785, Blast_Score=326, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=44.213649851632, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=40.3141361256544, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=43.75, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=43.8709677419355, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI32565909, Length=74, Percent_Identity=50, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=45.2127659574468, Blast_Score=326, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6323209, Length=371, Percent_Identity=45.2830188679245, Blast_Score=323, Evalue=3e-89,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=44.1489361702128, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=44.1489361702128, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=44.1489361702128, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=44.1489361702128, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=44.1489361702128, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=44.1489361702128, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=43.3510638297872, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=43.3510638297872, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580541, Length=364, Percent_Identity=42.5824175824176, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24580539, Length=116, Percent_Identity=43.1034482758621, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_AQUAE (O67222)

Other databases:

- EMBL:   AE000657
- PIR:   C70399
- RefSeq:   NP_213786.1
- ProteinModelPortal:   O67222
- SMR:   O67222
- GeneID:   1192568
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1154
- NMPDR:   fig|224324.1.peg.801
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   AAEO224324:AQ_1154-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41593; Mature: 41593

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLRMAESVTEGHPDKIADQLADALLDEFIKKDPYSKVSLEIMVTTGLVMVGGELTTES
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEECCCCCCHH
YVDIPRVVRSVIKDIGYTRPELGFDADTCAVVQSIDEQSPEIALGISSEGAGDTAIVVGY
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEE
ATKEAPNLMPWPITIAHKITKRISEYRKIGKFPFLRPDGKVLVAMIYEDGKPSYVQSIVA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHHHH
YVHHDPDVSINHLRELIIEEIIKKEIPEEFLTEKTSIKVNPTGRFVIGGPVADTGLTGRK
HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCEEEECCCCCCCCCCCHH
IVSDAYGDIGLSGGSAFSGKDPTKTDRSGSYLARMIAKHVVAGGWAERCLVQIGYAFGLT
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
EPVAFDIETFGTEKISKEILEDAVKKVFPLRPAEIIEFLDLRKPIYRQTSVYGHFGKENL
CCCEECHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PWEKLTKLEELKELLD
CHHHHHHHHHHHHHHC
>Mature Secondary Structure
MYNLRMAESVTEGHPDKIADQLADALLDEFIKKDPYSKVSLEIMVTTGLVMVGGELTTES
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEECCCCCCHH
YVDIPRVVRSVIKDIGYTRPELGFDADTCAVVQSIDEQSPEIALGISSEGAGDTAIVVGY
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEE
ATKEAPNLMPWPITIAHKITKRISEYRKIGKFPFLRPDGKVLVAMIYEDGKPSYVQSIVA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHHHH
YVHHDPDVSINHLRELIIEEIIKKEIPEEFLTEKTSIKVNPTGRFVIGGPVADTGLTGRK
HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCEEEECCCCCCCCCCCHH
IVSDAYGDIGLSGGSAFSGKDPTKTDRSGSYLARMIAKHVVAGGWAERCLVQIGYAFGLT
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
EPVAFDIETFGTEKISKEILEDAVKKVFPLRPAEIIEFLDLRKPIYRQTSVYGHFGKENL
CCCEECHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PWEKLTKLEELKELLD
CHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320