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The map label for this gene is dapA

Identifier: 15606400

GI number: 15606400

Start: 800897

End: 801781

Strand: Direct

Name: dapA

Synonym: aq_1143

Alternate gene names: 15606400

Gene position: 800897-801781 (Clockwise)

Preceding gene: 15606398

Following gene: 15606406

Centisome position: 51.63

GC content: 47.57

Gene sequence:

>885_bases
ATGTTTCAAGGCTCAATAGTTGCTCTGATTACTCCCTTCAAGGAAGGAGAAGTGGACTACGAAGCCCTCGGAAACCTAAT
TGAGTTTCACGTGGACAATGGTACAGACGCAATTTTGGTTTGCGGAACTACGGGAGAATCCCCGACCCTTACCTTTGAAG
AGCACGAAAAGGTTATAGAGTTTGCCGTAAAAAGGGCGGCGGGAAGGATTAAGGTAATCGCAGGAACGGGCGGGAACGCA
ACCCACGAAGCGGTTCACCTGACAGCCCATGCGAAAGAAGTAGGAGCGGACGGAGCCTTAGTGGTCGTTCCATACTACAA
CAAGCCCACACAAAGAGGTCTCTACGAGCACTTTAAAACGGTTGCTCAGGAAGTGGACATTCCCATAATCATTTACAACA
TACCCTCAAGAACCTGCGTGGAAATTTCCGTGGACACCATGTTCAAGCTCGCGAGCGAGTGCGAAAACATAGTCGCTTCA
AAGGAATCCACTCCCAACATGGATCGCATTTCGGAAATAGTAAAGAGGCTGGGAGAAAGTTTCAGCGTCCTTTCGGGAGA
TGACAGCCTGACTCTTCCCATGATGGCCCTCGGTGCTAAGGGAGTTATATCGGTTGCGAACAACGTAATGCCAAGAGAAG
TAAAAGAACTCATACGGGCCGCTTTGGAAGGAGACTTTAGAAGGGCGAGGGAAATCCATTACTACCTTCACGACCTATTT
AAGGTTCTCTTTATAGAAACGAATCCCATTCCCGTCAAAACCGCCTGCTGGATGCTGGGTATGTGTGAAAAGGAATTCAG
ACTTCCCTTAACGGAAATGTCTCCAGAGAATGAAAACAAGCTGAGAGAAGTCCTAAAGAAGTACAACCTACCTCTGAAAA
ACTAA

Upstream 100 bases:

>100_bases
CTTCTCTTTTCCAGAATTTTACGTGAAATATCCGCCCAGTTATTTCTCAGCGCTACCATAGCACAAAAATTTTATACTTG
TTAATTAAAAGGAGGAAAGG

Downstream 100 bases:

>100_bases
GCTTCCAGCCCTCTTGAAAGCCTCAACTTTGAGATGCATCTCCTCCGTTTCCCTTTCCTCTACCGAGTCCCACACTATAC
CGCATCCTGCGTAGTAATAA

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFQGSIVALITPFKEGEVDYEALGNLIEFHVDNGTDAILVCGTTGESPTLTFEEHEKVIEFAVKRAAGRIKVIAGTGGNA
THEAVHLTAHAKEVGADGALVVVPYYNKPTQRGLYEHFKTVAQEVDIPIIIYNIPSRTCVEISVDTMFKLASECENIVAS
KESTPNMDRISEIVKRLGESFSVLSGDDSLTLPMMALGAKGVISVANNVMPREVKELIRAALEGDFRRAREIHYYLHDLF
KVLFIETNPIPVKTACWMLGMCEKEFRLPLTEMSPENENKLREVLKKYNLPLKN

Sequences:

>Translated_294_residues
MFQGSIVALITPFKEGEVDYEALGNLIEFHVDNGTDAILVCGTTGESPTLTFEEHEKVIEFAVKRAAGRIKVIAGTGGNA
THEAVHLTAHAKEVGADGALVVVPYYNKPTQRGLYEHFKTVAQEVDIPIIIYNIPSRTCVEISVDTMFKLASECENIVAS
KESTPNMDRISEIVKRLGESFSVLSGDDSLTLPMMALGAKGVISVANNVMPREVKELIRAALEGDFRRAREIHYYLHDLF
KVLFIETNPIPVKTACWMLGMCEKEFRLPLTEMSPENENKLREVLKKYNLPLKN
>Mature_294_residues
MFQGSIVALITPFKEGEVDYEALGNLIEFHVDNGTDAILVCGTTGESPTLTFEEHEKVIEFAVKRAAGRIKVIAGTGGNA
THEAVHLTAHAKEVGADGALVVVPYYNKPTQRGLYEHFKTVAQEVDIPIIIYNIPSRTCVEISVDTMFKLASECENIVAS
KESTPNMDRISEIVKRLGESFSVLSGDDSLTLPMMALGAKGVISVANNVMPREVKELIRAALEGDFRRAREIHYYLHDLF
KVLFIETNPIPVKTACWMLGMCEKEFRLPLTEMSPENENKLREVLKKYNLPLKN

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=281, Percent_Identity=30.9608540925267, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI13540533, Length=299, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788823, Length=292, Percent_Identity=45.8904109589041, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI87082415, Length=290, Percent_Identity=31.0344827586207, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786463, Length=251, Percent_Identity=30.2788844621514, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789620, Length=234, Percent_Identity=28.2051282051282, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_AQUAE (O67216)

Other databases:

- EMBL:   AE000657
- PIR:   E70398
- RefSeq:   NP_213780.1
- PDB:   2EHH
- PDBsum:   2EHH
- ProteinModelPortal:   O67216
- SMR:   O67216
- GeneID:   1192562
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1143
- NMPDR:   fig|224324.1.peg.795
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- PhylomeDB:   O67216
- ProtClustDB:   PRK03170
- BioCyc:   AAEO224324:AQ_1143-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32670; Mature: 32670

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQGSIVALITPFKEGEVDYEALGNLIEFHVDNGTDAILVCGTTGESPTLTFEEHEKVIE
CCCCCEEEEEECCCCCCCCHHHHCCEEEEEECCCCCEEEEEECCCCCCCEEHHHHHHHHH
FAVKRAAGRIKVIAGTGGNATHEAVHLTAHAKEVGADGALVVVPYYNKPTQRGLYEHFKT
HHHHHCCCEEEEEEECCCCCCHHEEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
VAQEVDIPIIIYNIPSRTCVEISVDTMFKLASECENIVASKESTPNMDRISEIVKRLGES
HHHHCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
FSVLSGDDSLTLPMMALGAKGVISVANNVMPREVKELIRAALEGDFRRAREIHYYLHDLF
CEEECCCCCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KVLFIETNPIPVKTACWMLGMCEKEFRLPLTEMSPENENKLREVLKKYNLPLKN
HHHEECCCCCCHHHHHHHHHCCCHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFQGSIVALITPFKEGEVDYEALGNLIEFHVDNGTDAILVCGTTGESPTLTFEEHEKVIE
CCCCCEEEEEECCCCCCCCHHHHCCEEEEEECCCCCEEEEEECCCCCCCEEHHHHHHHHH
FAVKRAAGRIKVIAGTGGNATHEAVHLTAHAKEVGADGALVVVPYYNKPTQRGLYEHFKT
HHHHHCCCEEEEEEECCCCCCHHEEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
VAQEVDIPIIIYNIPSRTCVEISVDTMFKLASECENIVASKESTPNMDRISEIVKRLGES
HHHHCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
FSVLSGDDSLTLPMMALGAKGVISVANNVMPREVKELIRAALEGDFRRAREIHYYLHDLF
CEEECCCCCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KVLFIETNPIPVKTACWMLGMCEKEFRLPLTEMSPENENKLREVLKKYNLPLKN
HHHEECCCCCCHHHHHHHHHCCCHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320