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The map label for this gene is rhdA1 [H]

Identifier: 15606354

GI number: 15606354

Start: 746226

End: 747140

Strand: Direct

Name: rhdA1 [H]

Synonym: aq_1076

Alternate gene names: 15606354

Gene position: 746226-747140 (Clockwise)

Preceding gene: 15606353

Following gene: 15606355

Centisome position: 48.1

GC content: 42.3

Gene sequence:

>915_bases
ATGCTTTTACTGCTTCTTCTCGTCTCCATAGCGTTTTCAGTTCCCAAACTTGTAAGTCCGGAATGGTTGAAAGAAAACTT
AAGTAAAAAGAATTTGGTAATTATAGAATTCGGAGATACCCAGTCTTACCTTGTTGAAGGGCACATTCCGGGAGCGGTTT
TGACGGAAAAGGAGCAGTGGAGGAAGATGGATCCCGAAACGGGAGCTCTCGTGAGAAGAAGTGTGGAGTACTACGAGAAG
TTATTCAGGAGCTGGGGAATAAACAACGACTCGGAGGTGGTTATCTATTACAAGGGAAACACAAGGAACGAAATACTCGG
TGCAGTTTACGCCTACTGGGTATTTCACCTCCTCGGACATAAAAGGGTTGGGATACTTGACGGAGGTTGGAAGGAGTGGA
TAAAAAAGGGATACCCCGTGAGTTACGAAGAACCGGAAATAAACAGGGGAAACTTTAAGGCAAAGTACAATAAAGAACTT
GAAACTAACTGGAAGTACGTTTACGAAAATATAGGAAAAATACCCATAATTGACGGCAGATTGCCTGATTTTTACTTCGG
TATAGGAAAGTTTCCGGCAGCTAAAAAGTACGGACATATCCCCTGTTCTGTTCCTTTCTCATGGGAGTGGTGGGTAAAGA
GGGATAGAGAGACAGAAAAACTTTACATCGAATTTCCCTATTATGCAAAAGAGTTTTTAAGGAACCAAGGCTTAAAACCC
GAGGACGAGGTAATACTCTTTTGCTTCGGAGGAACTGGAGCCGCTTTCCTTTACTGGGTGTTTGACGTATCCGGACACAA
AAATATGAAGGTATACGACGCTTCAAAAAGGGAATGGGAAGCCCTCAACTTACCTTTAAACAGGTACGTATGGGAAACCT
TTAGGGATTGTAGAAAGTTCTTTTACACTAATTAA

Upstream 100 bases:

>100_bases
TTTTAAACTTTAGAATAGAGTTCTACAGGTACTTAAGGTACAAATCTCCGAAGTACGGTCCGAGTTTTAGGAGCGAATAC
TGGTTTATAGGGGTAGATTG

Downstream 100 bases:

>100_bases
GTATGACAGGGTATATTAGATTTTTTCTTGCTTTTCTAGTTTTAATTTCCCATTTAGGTATTAGGTTTAAAGGAATGAAT
GAAGGGGTTTTTGCTGTTGT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLLLLLLVSIAFSVPKLVSPEWLKENLSKKNLVIIEFGDTQSYLVEGHIPGAVLTEKEQWRKMDPETGALVRRSVEYYEK
LFRSWGINNDSEVVIYYKGNTRNEILGAVYAYWVFHLLGHKRVGILDGGWKEWIKKGYPVSYEEPEINRGNFKAKYNKEL
ETNWKYVYENIGKIPIIDGRLPDFYFGIGKFPAAKKYGHIPCSVPFSWEWWVKRDRETEKLYIEFPYYAKEFLRNQGLKP
EDEVILFCFGGTGAAFLYWVFDVSGHKNMKVYDASKREWEALNLPLNRYVWETFRDCRKFFYTN

Sequences:

>Translated_304_residues
MLLLLLLVSIAFSVPKLVSPEWLKENLSKKNLVIIEFGDTQSYLVEGHIPGAVLTEKEQWRKMDPETGALVRRSVEYYEK
LFRSWGINNDSEVVIYYKGNTRNEILGAVYAYWVFHLLGHKRVGILDGGWKEWIKKGYPVSYEEPEINRGNFKAKYNKEL
ETNWKYVYENIGKIPIIDGRLPDFYFGIGKFPAAKKYGHIPCSVPFSWEWWVKRDRETEKLYIEFPYYAKEFLRNQGLKP
EDEVILFCFGGTGAAFLYWVFDVSGHKNMKVYDASKREWEALNLPLNRYVWETFRDCRKFFYTN
>Mature_304_residues
MLLLLLLVSIAFSVPKLVSPEWLKENLSKKNLVIIEFGDTQSYLVEGHIPGAVLTEKEQWRKMDPETGALVRRSVEYYEK
LFRSWGINNDSEVVIYYKGNTRNEILGAVYAYWVFHLLGHKRVGILDGGWKEWIKKGYPVSYEEPEINRGNFKAKYNKEL
ETNWKYVYENIGKIPIIDGRLPDFYFGIGKFPAAKKYGHIPCSVPFSWEWWVKRDRETEKLYIEFPYYAKEFLRNQGLKP
EDEVILFCFGGTGAAFLYWVFDVSGHKNMKVYDASKREWEALNLPLNRYVWETFRDCRKFFYTN

Specific function: May be a sulfotransferase involved in the transport of sulfate. Displays very low rhodanese activity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=292, Percent_Identity=26.027397260274, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87082121, Length=285, Percent_Identity=24.9122807017544, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=215, Percent_Identity=26.046511627907, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559150, Length=274, Percent_Identity=27.007299270073, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 35951; Mature: 35951

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLLLLVSIAFSVPKLVSPEWLKENLSKKNLVIIEFGDTQSYLVEGHIPGAVLTEKEQW
CHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCEECCHHHH
RKMDPETGALVRRSVEYYEKLFRSWGINNDSEVVIYYKGNTRNEILGAVYAYWVFHLLGH
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCC
KRVGILDGGWKEWIKKGYPVSYEEPEINRGNFKAKYNKELETNWKYVYENIGKIPIIDGR
CEEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEECCC
LPDFYFGIGKFPAAKKYGHIPCSVPFSWEWWVKRDRETEKLYIEFPYYAKEFLRNQGLKP
CCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCEEEEEEECHHHHHHHHHCCCCC
EDEVILFCFGGTGAAFLYWVFDVSGHKNMKVYDASKREWEALNLPLNRYVWETFRDCRKF
CCCEEEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHH
FYTN
HCCC
>Mature Secondary Structure
MLLLLLLVSIAFSVPKLVSPEWLKENLSKKNLVIIEFGDTQSYLVEGHIPGAVLTEKEQW
CHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCEECCHHHH
RKMDPETGALVRRSVEYYEKLFRSWGINNDSEVVIYYKGNTRNEILGAVYAYWVFHLLGH
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCC
KRVGILDGGWKEWIKKGYPVSYEEPEINRGNFKAKYNKELETNWKYVYENIGKIPIIDGR
CEEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEECCC
LPDFYFGIGKFPAAKKYGHIPCSVPFSWEWWVKRDRETEKLYIEFPYYAKEFLRNQGLKP
CCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCEEEEEEECHHHHHHHHHCCCCC
EDEVILFCFGGTGAAFLYWVFDVSGHKNMKVYDASKREWEALNLPLNRYVWETFRDCRKF
CCCEEEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHH
FYTN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708376 [H]