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The map label for this gene is pncA [H]

Identifier: 15606301

GI number: 15606301

Start: 686941

End: 687537

Strand: Direct

Name: pncA [H]

Synonym: aq_994

Alternate gene names: 15606301

Gene position: 686941-687537 (Clockwise)

Preceding gene: 15606300

Following gene: 15606302

Centisome position: 44.28

GC content: 45.73

Gene sequence:

>597_bases
ATGAAGGTGAAGTTAACCGATAGAGACGCCCTTATAGTCGTTGATATGCAAAAGGACTTTATGCCCGGTGGTGCACTACC
CGTTCCAGAGGGAGACAAGATAATACCGAGGATAAACGAGTACGTCAAACTCTTTGAAAAGAAAGGACTTCCTGTTTTCT
ACACGAGGGACTGGCACCCAGAAAATCACATATCCTTCAAAGGACACGGCGGTATATGGCCACCTCACTGTGTTCAGGGA
ACGGAAGGTGCTAAGTTCCACGAAGACCTGTACATCCCCGCGGACAACAAGTTCATAATTTCAAAGGGAACTTCTCAGGA
GTTTGACGCGTATTCCGGTTTTCAGGGAACGATTTTAAATGACCTTTTGAAAGAGAGGGGAGTAAGAAGGATATTCGTGT
GCGGAGTGGCAACGGATTACTGCGTTAAAAACACAACGATAGGCGGTATAAACCTCGGCTACCAGGCCTTTGTTCTGGAA
GATGCGATAAAGGGCGTGGACGTGAACCCCGGAGACGTTGAGAGAGCAATAAACGAGATGATGGAAAGGGGAGCTGCCTT
TATAAAGATTGAAGATATAGTAGATTACAAAAAGTAA

Upstream 100 bases:

>100_bases
TGGAAGTTCTTCTTCGGAGTGAAGCCCCTCTTTGTTAGCGACTTAATAAAAATTTCTTGCTAAATTTATTTAATAGAAGT
TTTATCAAGGAGGTGGGAAT

Downstream 100 bases:

>100_bases
TTAAAAAAAATTGACATAATTTCACTAAGGTTGTATAATAAAAGGCAGGAAAGATAGGAGGTGATAAAATGGCTGAAAAG
AAGGAAAAAATAATTGGGAT

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKVKLTDRDALIVVDMQKDFMPGGALPVPEGDKIIPRINEYVKLFEKKGLPVFYTRDWHPENHISFKGHGGIWPPHCVQG
TEGAKFHEDLYIPADNKFIISKGTSQEFDAYSGFQGTILNDLLKERGVRRIFVCGVATDYCVKNTTIGGINLGYQAFVLE
DAIKGVDVNPGDVERAINEMMERGAAFIKIEDIVDYKK

Sequences:

>Translated_198_residues
MKVKLTDRDALIVVDMQKDFMPGGALPVPEGDKIIPRINEYVKLFEKKGLPVFYTRDWHPENHISFKGHGGIWPPHCVQG
TEGAKFHEDLYIPADNKFIISKGTSQEFDAYSGFQGTILNDLLKERGVRRIFVCGVATDYCVKNTTIGGINLGYQAFVLE
DAIKGVDVNPGDVERAINEMMERGAAFIKIEDIVDYKK
>Mature_198_residues
MKVKLTDRDALIVVDMQKDFMPGGALPVPEGDKIIPRINEYVKLFEKKGLPVFYTRDWHPENHISFKGHGGIWPPHCVQG
TEGAKFHEDLYIPADNKFIISKGTSQEFDAYSGFQGTILNDLLKERGVRRIFVCGVATDYCVKNTTIGGINLGYQAFVLE
DAIKGVDVNPGDVERAINEMMERGAAFIKIEDIVDYKK

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=204, Percent_Identity=37.2549019607843, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321401, Length=204, Percent_Identity=31.3725490196078, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648247, Length=204, Percent_Identity=30.3921568627451, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22224; Mature: 22224

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKLTDRDALIVVDMQKDFMPGGALPVPEGDKIIPRINEYVKLFEKKGLPVFYTRDWHP
CEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCC
ENHISFKGHGGIWPPHCVQGTEGAKFHEDLYIPADNKFIISKGTSQEFDAYSGFQGTILN
CCCEEECCCCCCCCCHHCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHH
DLLKERGVRRIFVCGVATDYCVKNTTIGGINLGYQAFVLEDAIKGVDVNPGDVERAINEM
HHHHHCCCEEEEEEECCCCHHCCCCEECCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHH
MERGAAFIKIEDIVDYKK
HHCCCEEEEEEHHCCCCC
>Mature Secondary Structure
MKVKLTDRDALIVVDMQKDFMPGGALPVPEGDKIIPRINEYVKLFEKKGLPVFYTRDWHP
CEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCC
ENHISFKGHGGIWPPHCVQGTEGAKFHEDLYIPADNKFIISKGTSQEFDAYSGFQGTILN
CCCEEECCCCCCCCCHHCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHH
DLLKERGVRRIFVCGVATDYCVKNTTIGGINLGYQAFVLEDAIKGVDVNPGDVERAINEM
HHHHHCCCEEEEEEECCCCHHCCCCEECCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHH
MERGAAFIKIEDIVDYKK
HHCCCEEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]