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The map label for this gene is gcsH3

Identifier: 15606264

GI number: 15606264

Start: 650617

End: 651102

Strand: Direct

Name: gcsH3

Synonym: aq_944

Alternate gene names: 15606264

Gene position: 650617-651102 (Clockwise)

Preceding gene: 15606263

Following gene: 15606265

Centisome position: 41.94

GC content: 48.56

Gene sequence:

>486_bases
ATGGGGAAGCAGGAGAAGGACTTAGGAACTGCTTGGGAGTATCAGGGTTGTCTTATTCCGAAAGATCTTTATTACGACAT
AGAAAATCAGGTCTGGGTAAGAGTAAACGAAGACGGAACGGTAACGCTCGGGCTCACGGACGTGGGACAGACCCGTGCCG
GTAGACTACTCCATATAAGGGTAAAACCCGTAGGGACGAAAGTAAAGAAGGGAAAACCAGTTGCGACACTTGAAAGCGGG
AAGTGGGCCGGACCCGTTCCCGCACTCGTGGAGGGCGAAATCGTTGAAGTAAACCCGAAAGTCGTGGAAGACCCGAACTA
CATAAACATTGACCCTTACGGAGATGCGTGGATCGTAAAGATTAAACCCACAAGCGAAGAAACGCTCAAGAGAGACCTCT
CCGAACTTGCTCACGGTGAAAAAGCTCACGAAGAAATGAAAAAGCATATAGACGAGTGGGATATAGTTTGTATGAGGTGT
GTGTGA

Upstream 100 bases:

>100_bases
ACCCGTGAGGAAAGTAAGCCTCAAATAAGAAATTCTTATATCCTTATAATGATAAGTAAGTGTTGATTTACTTTGATAAA
GGTCATAATATTAATATCAA

Downstream 100 bases:

>100_bases
TGGGTGCGAAGGATAAACACGTTATTCTTCTGGTTTCTCAATGGTGTCCTACATGTCCTCATGCGGATGCCCTCTGGAAA
AAACTTCAGGAAGAATACGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MGKQEKDLGTAWEYQGCLIPKDLYYDIENQVWVRVNEDGTVTLGLTDVGQTRAGRLLHIRVKPVGTKVKKGKPVATLESG
KWAGPVPALVEGEIVEVNPKVVEDPNYINIDPYGDAWIVKIKPTSEETLKRDLSELAHGEKAHEEMKKHIDEWDIVCMRC
V

Sequences:

>Translated_161_residues
MGKQEKDLGTAWEYQGCLIPKDLYYDIENQVWVRVNEDGTVTLGLTDVGQTRAGRLLHIRVKPVGTKVKKGKPVATLESG
KWAGPVPALVEGEIVEVNPKVVEDPNYINIDPYGDAWIVKIKPTSEETLKRDLSELAHGEKAHEEMKKHIDEWDIVCMRC
V
>Mature_160_residues
GKQEKDLGTAWEYQGCLIPKDLYYDIENQVWVRVNEDGTVTLGLTDVGQTRAGRLLHIRVKPVGTKVKKGKPVATLESGK
WAGPVPALVEGEIVEVNPKVVEDPNYINIDPYGDAWIVKIKPTSEETLKRDLSELAHGEKAHEEMKKHIDEWDIVCMRCV

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=36.6666666666667, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=32.7731092436975, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319272, Length=103, Percent_Identity=30.0970873786408, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH3_AQUAE (O67080)

Other databases:

- EMBL:   AE000657
- PIR:   E70381
- RefSeq:   NP_213643.1
- ProteinModelPortal:   O67080
- SMR:   O67080
- GeneID:   1193622
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_944
- NMPDR:   fig|224324.1.peg.658
- HOGENOM:   HBG693789
- OMA:   IANRDPY
- ProtClustDB:   PRK13380
- BioCyc:   AAEO224324:AQ_944-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 18106; Mature: 17975

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQEKDLGTAWEYQGCLIPKDLYYDIENQVWVRVNEDGTVTLGLTDVGQTRAGRLLHIR
CCCCCCCCCCEECCCCEECCHHHEEECCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEE
VKPVGTKVKKGKPVATLESGKWAGPVPALVEGEIVEVNPKVVEDPNYINIDPYGDAWIVK
EECCCCCCCCCCCEEEECCCCCCCCCCCEECCEEEEECCEEECCCCEEEECCCCCEEEEE
IKPTSEETLKRDLSELAHGEKAHEEMKKHIDEWDIVCMRCV
EECCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
GKQEKDLGTAWEYQGCLIPKDLYYDIENQVWVRVNEDGTVTLGLTDVGQTRAGRLLHIR
CCCCCCCCCEECCCCEECCHHHEEECCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEE
VKPVGTKVKKGKPVATLESGKWAGPVPALVEGEIVEVNPKVVEDPNYINIDPYGDAWIVK
EECCCCCCCCCCCEEEECCCCCCCCCCCEECCEEEEECCEEECCCCEEEECCCCCEEEEE
IKPTSEETLKRDLSELAHGEKAHEEMKKHIDEWDIVCMRCV
EECCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320