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The map label for this gene is queF

Identifier: 15606257

GI number: 15606257

Start: 643202

End: 643591

Strand: Direct

Name: queF

Synonym: aq_931

Alternate gene names: 15606257

Gene position: 643202-643591 (Clockwise)

Preceding gene: 15606256

Following gene: 15606261

Centisome position: 41.46

GC content: 44.62

Gene sequence:

>390_bases
ATGGAGGCAAAGGAGAAGAAGTACGGAGAAATTGAGATCGAAAAGGCACAGCTCGAGGCATGGCCGAATCCAAATCCCGA
AAGGGATTACATGATAGAGATAACATTTCCTGAATTTACGTGCCTGTGTCCGCGTTCAGGTTATCCAGACTTTGCAACGA
TAAAAATCCGCTACATTCCCGACAAGTACATAGTTGAGTTAAAGTCCTTAAAGCTCTGGCTCAATAAGTTCAGGAACAGG
TACATATCCCACGAGGCGGCTACAAACGAGATATACCAAGCCCTTTACGACCTTCTTAAACCCAGATTCTTGGAAGTTGT
GGGAGACTTTCATCCGAGGGGTAATGTGCATACCGTTGTAAGGGTAAGGAGTGATGAGAATTACGGATAA

Upstream 100 bases:

>100_bases
AAGAAATCGCGGGTGAAGTTAAAGAACTAAAAGAACTCCTCTACAAGGTTCTGGAGGCGGAAGCTAAAAAAGAAGTTTAA
AAGTTCTATCTTTAATCTTT

Downstream 100 bases:

>100_bases
TGGCGGAGGGGGAGGGATTCGAACCCTCGGAGGGGTTTTAGCCCCTCTGCCGCTTAGCAAGCGGCCGCCTTCGGCCTCTC
GGCCACCCCTCCTTTACAAA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MEAKEKKYGEIEIEKAQLEAWPNPNPERDYMIEITFPEFTCLCPRSGYPDFATIKIRYIPDKYIVELKSLKLWLNKFRNR
YISHEAATNEIYQALYDLLKPRFLEVVGDFHPRGNVHTVVRVRSDENYG

Sequences:

>Translated_129_residues
MEAKEKKYGEIEIEKAQLEAWPNPNPERDYMIEITFPEFTCLCPRSGYPDFATIKIRYIPDKYIVELKSLKLWLNKFRNR
YISHEAATNEIYQALYDLLKPRFLEVVGDFHPRGNVHTVVRVRSDENYG
>Mature_129_residues
MEAKEKKYGEIEIEKAQLEAWPNPNPERDYMIEITFPEFTCLCPRSGYPDFATIKIRYIPDKYIVELKSLKLWLNKFRNR
YISHEAATNEIYQALYDLLKPRFLEVVGDFHPRGNVHTVVRVRSDENYG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_AQUAE (O67073)

Other databases:

- EMBL:   AE000657
- PIR:   F70380
- RefSeq:   NP_213635.1
- ProteinModelPortal:   O67073
- SMR:   O67073
- GeneID:   1193615
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_931
- NMPDR:   fig|224324.1.peg.650
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- BioCyc:   AAEO224324:AQ_931-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 15331; Mature: 15331

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKEKKYGEIEIEKAQLEAWPNPNPERDYMIEITFPEFTCLCPRSGYPDFATIKIRYIP
CCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEEEECC
DKYIVELKSLKLWLNKFRNRYISHEAATNEIYQALYDLLKPRFLEVVGDFHPRGNVHTVV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEE
RVRSDENYG
EEECCCCCC
>Mature Secondary Structure
MEAKEKKYGEIEIEKAQLEAWPNPNPERDYMIEITFPEFTCLCPRSGYPDFATIKIRYIP
CCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEEEECC
DKYIVELKSLKLWLNKFRNRYISHEAATNEIYQALYDLLKPRFLEVVGDFHPRGNVHTVV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEE
RVRSDENYG
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320