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The map label for this gene is genX

Identifier: 15606147

GI number: 15606147

Start: 528310

End: 529191

Strand: Direct

Name: genX

Synonym: aq_763

Alternate gene names: 15606147

Gene position: 528310-529191 (Clockwise)

Preceding gene: 15606146

Following gene: 15606149

Centisome position: 34.06

GC content: 44.67

Gene sequence:

>882_bases
ATGGATTTGTTAGATGCTTGGGATTACTTCATAAACGAGGTTAGAAAATTTTTCAAAGAGAAGGGCTACACCGAGGTCTC
CACCCCCCTCCTTCTCGACTTTCCCAACTTAGACTCAAACGTAGAGCCCGTAAAGGTGGAAGTTCTGGAAAGGGGAGAGA
ATAAAGTAAAGTGGCTTCACACGTCCCCCGAATACTCTATGAAAAAGCTCCTTTCCCGCTACAAAAGGGATATATTCCAG
ATAACAAAAGTCTTCAGGAACAACGAGTGGGGTAGGCTTCACAGGATAGAGTTTCACATGCTCGAGTGGTACGCTGTAGG
TTGCGACTACCTTTACTTAATAGAGGAGCTCAAACAGCTCCTCAATAAACTCTTTGGCTTCAAAGAATTTGAAGTTATTA
CGGTGGAGGAAGCCTTTAAAAGGCACTTCGGGGAGGGAATTCCGCAGGAAGAAAGCAGTATGAAAGAACTCCTTGAGAGA
AAGGGTATTGATTTTTCAGAGGACGAAGACTGGGAAACGCTTTTCTACAGGGCTTTTATAGAAGTTGAGAGGCATTTAGG
TTTTAATAGACCCACTTTTTTGATAAACTTCCCGGAAAGGCTGTGCGCCCTCGCAAAAGTAAGAAACGGATATGCGGAGA
GGTTTGAACTCTTTATAAAGGGAATAGAACTCGCAAACGGCTGGACCGAGGAGACCAACCCTGAGGAAGTGAGAAAAAGG
CTTGAGAGGGAAGCAAAAAAGAGAAATCTTCCCTTGGACGAGGACTTTATAAAGGCTCACGAAGATATGCCCGAGTGTGC
GGGATGCTCCCTCGGTATAGACAGGCTCTTTTCCCTCTTTTTAGGAAAGGAAGAACTTGTCAGCGAATTCTTCCGAGCTT
AA

Upstream 100 bases:

>100_bases
AGAAATTCAAACCCATAACCGTAGGTCAGGCCTCCAGAATTGACGGAATTACGCCCGCAGCGATAACGGCACTACTCGTT
TACCTCGGAAAGTTAGACTG

Downstream 100 bases:

>100_bases
CGGATATCTTTTGAGCGCTTTCTTTCAGTATTTCCTTAACCCAGCCCGTTAGGTCTTCTTTGGTCATCCTGTAGGAAGGG
GCAACGATGGTGACAGTGTA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLLDAWDYFINEVRKFFKEKGYTEVSTPLLLDFPNLDSNVEPVKVEVLERGENKVKWLHTSPEYSMKKLLSRYKRDIFQ
ITKVFRNNEWGRLHRIEFHMLEWYAVGCDYLYLIEELKQLLNKLFGFKEFEVITVEEAFKRHFGEGIPQEESSMKELLER
KGIDFSEDEDWETLFYRAFIEVERHLGFNRPTFLINFPERLCALAKVRNGYAERFELFIKGIELANGWTEETNPEEVRKR
LEREAKKRNLPLDEDFIKAHEDMPECAGCSLGIDRLFSLFLGKEELVSEFFRA

Sequences:

>Translated_293_residues
MDLLDAWDYFINEVRKFFKEKGYTEVSTPLLLDFPNLDSNVEPVKVEVLERGENKVKWLHTSPEYSMKKLLSRYKRDIFQ
ITKVFRNNEWGRLHRIEFHMLEWYAVGCDYLYLIEELKQLLNKLFGFKEFEVITVEEAFKRHFGEGIPQEESSMKELLER
KGIDFSEDEDWETLFYRAFIEVERHLGFNRPTFLINFPERLCALAKVRNGYAERFELFIKGIELANGWTEETNPEEVRKR
LEREAKKRNLPLDEDFIKAHEDMPECAGCSLGIDRLFSLFLGKEELVSEFFRA
>Mature_293_residues
MDLLDAWDYFINEVRKFFKEKGYTEVSTPLLLDFPNLDSNVEPVKVEVLERGENKVKWLHTSPEYSMKKLLSRYKRDIFQ
ITKVFRNNEWGRLHRIEFHMLEWYAVGCDYLYLIEELKQLLNKLFGFKEFEVITVEEAFKRHFGEGIPQEESSMKELLER
KGIDFSEDEDWETLFYRAFIEVERHLGFNRPTFLINFPERLCALAKVRNGYAERFELFIKGIELANGWTEETNPEEVRKR
LEREAKKRNLPLDEDFIKAHEDMPECAGCSLGIDRLFSLFLGKEELVSEFFRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI87082379, Length=294, Percent_Identity=35.0340136054422, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1790571, Length=299, Percent_Identity=28.7625418060201, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789256, Length=303, Percent_Identity=29.3729372937294, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJEA_AQUAE (O66963)

Other databases:

- EMBL:   AE000657
- PIR:   H70366
- RefSeq:   NP_213524.1
- ProteinModelPortal:   O66963
- GeneID:   1193413
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_763
- NMPDR:   fig|224324.1.peg.539
- HOGENOM:   HBG631383
- OMA:   PESHIDP
- PhylomeDB:   O66963
- ProtClustDB:   PRK09350
- BioCyc:   AAEO224324:AQ_763-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00174
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- PANTHER:   PTHR22594
- TIGRFAMs:   TIGR00462

Pfam domain/function: PF00152 tRNA-synt_2

EC number: 6.1.1.6

Molecular weight: Translated: 35013; Mature: 35013

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLDAWDYFINEVRKFFKEKGYTEVSTPLLLDFPNLDSNVEPVKVEVLERGENKVKWLH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEEE
TSPEYSMKKLLSRYKRDIFQITKVFRNNEWGRLHRIEFHMLEWYAVGCDYLYLIEELKQL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LNKLFGFKEFEVITVEEAFKRHFGEGIPQEESSMKELLERKGIDFSEDEDWETLFYRAFI
HHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EVERHLGFNRPTFLINFPERLCALAKVRNGYAERFELFIKGIELANGWTEETNPEEVRKR
HHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LEREAKKRNLPLDEDFIKAHEDMPECAGCSLGIDRLFSLFLGKEELVSEFFRA
HHHHHHHCCCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MDLLDAWDYFINEVRKFFKEKGYTEVSTPLLLDFPNLDSNVEPVKVEVLERGENKVKWLH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEEE
TSPEYSMKKLLSRYKRDIFQITKVFRNNEWGRLHRIEFHMLEWYAVGCDYLYLIEELKQL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LNKLFGFKEFEVITVEEAFKRHFGEGIPQEESSMKELLERKGIDFSEDEDWETLFYRAFI
HHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EVERHLGFNRPTFLINFPERLCALAKVRNGYAERFELFIKGIELANGWTEETNPEEVRKR
HHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LEREAKKRNLPLDEDFIKAHEDMPECAGCSLGIDRLFSLFLGKEELVSEFFRA
HHHHHHHCCCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320